PPM1D (NM_003620) Human Untagged Clone

SKU SC117895

PPM1D (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPM1D
• Synonyms: IDDGIP; JDVS; PP2C-DELTA; WIP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003620, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACG; TTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCA; GCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGGTG; GCAGCCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCC; GCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCG; GGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT; GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGGCCAAAGACTA; TGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGGCATGAAGATGTATGT; AGCTCACGTAGGTGACTCAGGGGTGGTTCTTGGAATTCAGGATGACCCGAAGGATGACTTTGTCAGAGCT; GTGGAGGTGACACAGGACCATAAGCCAGAACTTCCCAAGGAAAGAGAACGAATCGAAGGACTTGGTGGGA; GTGTAATGAACAAGTCTGGGGTGAATCGTGTAGTTTGGAAACGACCTCGACTCACTCACAATGGACCTGT; TAGAAGGAGCACAGTTATTGACCAGATTCCTTTTCTGGCAGTAGCAAGAGCACTTGGTGATTTGTGGAGC; TATGATTTCTTCAGTGGTGAATTTGTGGTGTCACCTGAACCAGACACAAGTGTCCACACTCTTGACCCTC; AGAAGCACAAGTATATTATATTGGGGAGTGATGGACTTTGGAATATGATTCCACCACAAGATGCCATCTC; AATGTGCCAGGACCAAGAGGAGAAAAAATACCTGATGGGTGAGCATGGACAATCTTGTGCCAAAATGCTT; GTGAATCGAGCATTGGGCCGCTGGAGGCAGCGTATGCTCCGAGCAGATAACACTAGTGCCATAGTAATCT; GCATCTCTCCAGAAGTGGACAATCAGGGAAACTTTACCAATGAAGATGAGTTATACCTGAACCTGACTGA; CAGCCCTTCCTATAATAGTCAAGAAACCTGTGTGATGACTCCTTCCCCATGTTCTACACCACCAGTCAAG; TCACTGGAGGAGGATCCATGGCCAAGGGTGAATTCTAAGGACCATATACCTGCCCTGGTTCGTAGCAATG; CCTTCTCAGAGAATTTTTTAGAGGTTTCAGCTGAGATAGCTCGAGAGAATGTCCAAGGTGTAGTCATACC; CTCAAAAGATCCAGAACCACTTGAAGAAAATTGCGCTAAAGCCCTGACTTTAAGGATACATGATTCTTTG; AATAATAGCCTTCCAATTGGCCTTGTGCCTACTAATTCAACAAACACTGTCATGGACCAAAAAAATTTGA; AGATGTCAACTCCTGGCCAAATGAAAGCCCAAGAAATTGAAAGAACCCCTCCAACAAACTTTAAAAGGAC; ATTAGAAGAGTCCAATTCTGGCCCCCTGATGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGT; GCTCAGCCTGCAAGTCTCCCCACAACCTCACAGCGAAAGAACTCTGTTAAACTCACCATGCGACGCAGAC; TTAGGGGCCAGAAGAAAATTGGAAATCCTTTACTTCATCAACACAGGAAAACTGTTTGTGTTTGCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003620 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTCTCCGGGTCCGCC; CCCTCCCCCTTCTCGGCGTCGTCGAAGATAAACAATAGTTGGCCGGCGAGCGCCTAGTGT; GTCTCCCGCCGCCGGATTCGGCGGGCTGCGTGGGACCGGCGGGATCCCGGCCAGCCGGCC; ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTAC; ATGGAGGACGTTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCG; CCGCGGCGGTCGCTGTCTCAGCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGC; GGCGAAGTCTCGGGGAAAGGCCCAGCGGTGGCAGCCCGAGAGGCTCGCGACCCTCTCCCG; GACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCCGCCGTTCCTCCGTGGCCTTT; TTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTG; TGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT; GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGG; CCAAAGACTATGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATT; CGGNGCATGAAGATGTATGTAGCTCACGTAGGTGACTCAGGGNGTGGCTTCTTGGNATTC; AGGATGACCCGAAGGATGACTNTGTCAGAGCTGTGGGAGTGACACAGGACCCATAGCCAG; AACTTCCCAGGNNAAGAGACGAATCGATGACTTGGTGGGAGTGTAAGAACAGTCCTGGGT; GAATCGCGGTATTTGGGAACCGACTCGACTACTCACATGGACCTGNTAGAAGAGCCAGTT; ATGACAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003620 unedited; ACCGCGGCCGCAATTTAGAGTCGAGCCCTTTTCTTTTTTTTTTTCTTTTTCTTTTTTTTT; TTTTTTTTTTTTTTTCTTTTTGGGGGACCGGTCACTGGTTTATTAAAAGAACCTTTTCCC; CTATAATCAATGAAAACTGTTTGATCTAAAAAAATTTAAAAAATGGCGGGAATTAACCGC; CCCCTTTGTTTTTGAAATAACAAGGACAATTTCCCCTTTTTTAACCCAACCCGAAAAGGG; GGTAATTCCTTTAAAACCTAAATATGCCATTGGTCTGGGATGAAAAAAAAGGGAATTGCC; CTTAAACTGTTTTAAGGCAACACCCTTCCTTTTATGCCGAAAACTTAATAAGCCCTTTCG; CAGACCCAGGCTTTAAAAAAACGCCCCTGGTGGAAAACACACCGGCTAGCCCCTTAATTG; CGCCAACACCAACTGGTCTCGGCGTTCATCCTTCTATGAAACATTTACCTCCTAATTCCG; CCATTCCGAAATCAAAACTTTTGTTTAACCGACCCCTTGGCTTCTTCAAAACATTAAGCG; ACACCCCCGCCGCCCTGAAAATACACCACTTTTCCCCCCCGCCTAATAATTTACCTTACA; AAGTCGTACCGCTTCCAACACAACGCTCCATACAAATTCTCTACCTACCCCTCTCACACG; TCTACCCCGTTTTTCAAAACCCAACAAAATCTTTGGATTTCTCCCAACCCCCATCCCTTT; TGTACCCCCTCCTCGTATTACACACCTTTAACTCCGGTCCTTCCACACTGAATTTCGCGC; CTTCCCACTCCCCCATTTCTCGCGTTCTTTCCTTCACCCTTTAACCCCTTTCCCTTTACC; CCCCCCCGCCCGATAAACCGCCCATCCAAAAGAAACATGTTCTCCTTTATCCCCCCCTCA; AAAAGGTACACTGCCCCACTTAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_003620
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003620.2, NP_003611.1
• RefSeq Size: 3163 bp
• RefSeq ORF: 1818 bp
• Locus ID: 8493
• UniProt ID: O15297
• Cytogenetics: 17q23.2
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: p53 signaling pathway
• Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]