RAE1 (NM_003610) Human Untagged Clone

SKU SC117882

RAE1 (untagged)-Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAE1
• Synonyms: dJ481F12.3; dJ800J21.1; Gle2; MIG14; Mnrp41; MRNP41
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117882 sequence for NM_003610 edited (data generated by NextGen Sequencing); ATGAGCCTGTTTGGAACAACCTCAGGTTTTGGAACCAGTGGGACCAGCATGTTTGGCAGT; GCAACTACAGACAATCACAATCCCATGAAGGATATTGAAGTAACATCATCTCCTGATGAT; AGCATTGGTTGTCTGTCTTTTAGCCCACCAACCTTGCCGGGGAACTTTCTTATTGCAGGA; TCATGGGCTAATGATGTTCGCTGCTGGGAAGTTCAAGACAGTGGACAGACCATTCCAAAA; GCCCAGCAGATGCACACTGGGCCTGTGCTTGATGTCTGCTGGAGTGACGATGGGAGCAAA; GTGTTTACGGCATCGTGTGATAAAACTGCCAAAATGTGGGACCTCAGCAGTAACCAAGCG; ATACAGATCGCACAGCATGATGCTCCTGTTAAAACCATCCATTGGATCAAAGCTCCAAAC; TACAGCTGTGTGATGACTGGGAGCTGGGATAAGACTTTAAAGTTTTGGGATACTCGATCG; TCAAATCCTATGATGGTTTTGCAACTCCCTGAAAGGTGTTACTGTGCTGACGTGATATAC; CCCATGGCTGTGGTGGCAACTGCAGAGAGGGGCCTGATTGTCTATCAGCTAGAGAATCAA; CCTTCTGAATTCAGGAGGATAGAATCTCCACTGAAACATCAGCATCGGTGTGTGGCTATT; TTTAAAGACAAACAGAACAAGCCTACTGGTTTTGCCCTGGGAAGTATCGAGGGGAGAGTT; GCTATTCACTATATCAACCCCCCGAACCCCGCCAAAGATAACTTCACCTTTAAATGTCAT; CGATCTAATGGAACCAACACTTCAGCTCCTCAGGACATTTATGCGGTAAATGGAATCGCG; TTCCATCCTGTTCATGGCACCCTTGCAACTGTGGGATCTGATGGTAGATTCAGCTTCTGG; GACAAAGATGCCAGAACAAAACTAAAAACTTCGGAACAGTTAGATCAGCCCATCTCAGCT; TGCTGTTTCAATCACAATGGAAACATATTTGCATACGCTTCCAGCTACGACTGGTCAAAG; GGACATGAATTTTATAATCCCCAGAAAAAAAATTACATTTTCCTGCGTAATGCAGCCGAA; GAGCTAAAGCCCAGGAATAAGAAGTAG; ; Clone variation with respect to NM_003610.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003610 unedited; GGTCAGGAATTNTGAAAACGACTTACTATAGGCGGCCCGCGAATCGGCACGGAGGGGTAG; TCAGGGCAGTTTCTACCGCAGGCTTAAGGAGGCTTCGGGCTCCTGGGATTTCTGTCCGCG; CTCCTGGCCCTCGTCCTTCGCGCCAGAGCAGGTTCGCAAACTCCTCAGACCCTTCTGCTC; CCGGCCGCCGCTTTCCGCCGGGGCGAGACCCCCAGGTTCAAAATGAGCCTGTTTGGAACA; ACCTCAGGTTTTGGAACCAGTGGGACCAGCATGTTTGGCAGTGCAACTACAGACAATCAC; AATCCCATGAAGGATATTGAAGTAACATCATCTCCTGATGATAGCATTGGTTGTCTGTCT; TTTAGCCCACCAACCTTGCCGGGGAACTTTCTTATTGCAGGATCATGGGCTAATGATGTT; CGCTGCTGGGAAGTTCAAGACAGTGGACAGACCATTCCAAAAGCCCAGCAGATGCACACT; GGGCCTGTGCTTGATGTCTGCTGGAGTGACGATGGGAGCAAAGTGTTTACGGCATCGTGT; GATAAAACTGCCAAAATGTGGGACCTCAGCAGTAACCAAGCGATACAGATCGCACAGCAT; GATGCTCCTGTTAAAACCATCCATTGGATCAAAGCTCCAAACTACAGCTGTGTGATGACT; GGGAGCTGGGATAAGACTTTAAAGTTTTGGGATACTCGATCGTCAAATCCTATGATGGTT; TTGCAACTCCCTGAAAGGTGTTACTGTGCTGACGTGATATACCCCATGGCCTGTGGTGGC; AACTGCAGAGAGGGGCCTGATTGTCTATCAGCTAGAGAATCAACCTTCTGAATTCAGGAG; GATAGAATCTCCACTGGAACATCAGCATCGGNTGTGTGGCTATNTTTTAAGACAACAGAA; CAGCCCTACTGGNNTTGCCCTGGGAGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003610 unedited; GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCAATCTGCAAAAAACTTTA; TTACAAAATACAGCACGGGGCTCACAGACGCTTTTCCAATATTCTCTAACACGTACACTG; AAGCGCTGCAAAATCCCTGGCGGTGCCCGCAATCTAACGTCTACAATAACCTCAACCCCC; TTAGTTACAGAAAAACTCTGCAACAGGCAGTGGAATGGAGAGACGGCAAGTCGCCGCGGA; CGCCTGGGCTCTCAGCTGCTGAACAATGACATCGTTTTCTCCAGGGGTTGAAATCCATGT; CCATGGCTGACAACCCAACAAGGCTGGGACCCAAATTCGTACAGAGATGAGGCAGAGTGG; AGAGAAACAACTCTGGCTGAGCCAGAGTCTCCAGCCACTACTTCTTATTCCTGGGCTTTA; GCTCTTCGGCTGCATTACGCAGGAAAATGTAATTTTTTTTCTGGGGATTATAAAATTCAT; GTCCCTTTGACCAGTCGTAGCTGGAAGCGTATGCAAATATGTTTCCATTGTGATTGAAAC; AGCAAGCTGAGATGGGCTGATCTAACTGTTCCGAAGTTTTTAGTTTTGTTCTGGCATCTT; TGTCCCAGAAGCTGAATCTACCATCAGATCCCACAGTTGCAAGGGTGCCATGAACAGGAT; GGAACGCGATTCCATTTACCGCATAAATGTCCTGAGGAGCTGAAGTGTTGGTTCCATTAN; ATCGATGACATTTAAAGGTGAAGTTATCTTTGGCGGGGGTCGGNGGGTTGATATAGTGAA; TAGCAACTCTNCCCTCGATACTTCCNAGGGCAAAACCAGTAGGCTTGGTCTGTTTGTCTT; AAAAATACCACACACGATGCTGATTTTCAGTGGAGATNCTACCTCCTGAATCAAAAGGGT; GATCTTAGCTGATGACATCAGGCCCTCTCTGCAGTGCCACACACCATGGTATTTACGTAG; CAGTAAACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003610
• Insert Size: 1830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003610.3, NP_003601.1
• RefSeq Size: 1815 bp
• RefSeq ORF: 1107 bp
• Locus ID: 8480
• UniProt ID: P78406
• Cytogenetics: 20q13.31
• Domains: WD40
• Summary: Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) differs from variant 2 in the 5' UTR and encodes the same protein as variant 2.