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TPST1 (NM_003596) Human Untagged Clone

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SKU
SC117873
TPST1 (untagged)-Human tyrosylprotein sulfotransferase 1 (TPST1)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TPST1
Synonyms TANGO13A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117873 sequence for NM_003596 edited (data generated by NextGen Sequencing)
ATGGTTGGAAAGCTGAAGCAGAACTTACTATTGGCATGTCTGGTGATTAGTTCTGTGACT
GTGTTTTACCTGGGCCAGCATGCCATGGAATGCCATCACCGGATAGAGGAACGTAGCCAG
CCAGTCAAATTGGAGAGCACAAGGACCACTGTGAGAACTGGCCTGGACCTCAAAGCCAAC
AAAACCTTTGCCTATCACAAAGATATGCCTTTAATATTTATTGGAGGTGTGCCTCGGAGT
GGAACCACACTCATGAGGGCCATGCTGGACGCACATCCTGACATTCGCTGTGGAGAGGAA
ACCAGGGTCATTCCCCGAATCCTGGCCCTGAAGCAGATGTGGTCACGGTCAAGTAAAGAG
AAGATCCGCCTGGATGAGGCTGGTGTTACTGATGAAGTGCTGGATTCTGCCATGCAAGCC
TTCTTACTAGAAATTATCGTTAAGCATGGGGAGCCAGCCCCTTATTTATGTAATAAAGAT
CCTTTTGCCCTGAAATCTTTAACTTACCTTTCTAGGTTATTCCCCAATGCCAAATTTCTC
CTGATGGTCCGAGATGGCCGGGCATCAGTACATTCAATGATTTCTCGAAAAGTTACTATA
GCTGGATTTGATCTGAACAGCTATAGGGACTGTTTGACAAAGTGGAATCGTGCTATAGAG
ACCATGTATAACCAGTGTATGGAGGTTGGTTATAAAAAGTGCATGTTGGTTCACTATGAA
CAACTTGTCTTACATCCTGAACGGTGGATGAGAACACTCTTAAAGTTCCTCCAGATTCCA
TGGAACCACTCAGTATTGCACCATGAAGAGATGATTGGGAAAGCTGGGGGAGTGTCTCTG
TCAAAAGTGGAGAGATCTACAGACCAAGTAATCAAGCCAGTCAATGTAGGAGCTCTATCA
AAATGGGTTGGGAAGATACCGCCAGATGTTTTACAAGACATGGCAGTGATTGCTCCTATG
CTTGCCAAGCTTGGATATGACCCATATGCCAACCCACCTAACTACGGAAAACCTGATCCC
AAAATTATTGAAAACACTCGAAGGGTCTATAAGGGAGAATTCCAACTACCTGACTTTCTT
AAAGAAAAACCACAGACTGAGCAAGTGGAGTAG

Clone variation with respect to NM_003596.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003596 unedited
ATCCAAACTACTATAGGCGGCCGCCAATTCGGCACGAGGGGGAGACGCTGTTGAGGCGGC
TTCGGTTGCGGGTCGGAACGGCGCTGCTCTGCGGGGCCGGTCCAGGCTGGCAGCTGCCGG
CGCTTGGCGGTGAGGGCGGGCTCCCGAGTGGCCCCCCACCGAAGGCGGTGGGACCAGCGG
CTGAGGCCAGGATGCCGTCCAGGCGGCGCGGCGGCTCCTCACTCATCCCAGATGTTGGTT
ATCTTTCTGAAGTAGACTGTCCATGGCCTGAACATTTTCCGAAAATCATTTTGAGCAAAA
TATCTGTTTAATAACAAGATAACCACATCAAGATGGTTGGAAAGCTGAAGCAGAACTTAC
TATTGGCATGTCTGGTGATTAGTTCTGTGACTGTGTTTTACCTGGGCCAGCATGCCATGG
AATGCCATCACCGGATAGAGGAACGTAGCCAGCCAGTCAAATTGGAGAGCACAAGGACCA
CTGTGAGAACTGGCCTGGACCTCAAAGCCAACAAAACCTTTGCCTATCACAAAGATATGC
CTTTAATATTTATTGGAGGTGTGCCTCGGAGTGGAACCACACTCATGAGGGCCATGCTGG
ACGCACATCCTGACATTCGCTGTGGAGAGGAAACCAGGGTCATTCCCCGAATCCTGGCCC
TGAAGCAGATGTGGTCACGGTCAAGTAAAGAGAAGATCCGNCTGGATGAGGCTGGTGTTA
CTGATGAAGTGCTGGATTCTGCCATGCAAGCCTTTCTACTAGAAATTATCGTTAAGCATG
GGGAGCCAGCCCCTTATTTATGTATAAAGATCCTTTTGCCCTGAAATCTTTACTTACCTT
TCTAGGTTATTCCCCATGCCAAATTCTNCTGATGGTCCGAGATGCCGGGCATCATACATT
CATGATTCTCGAAAGTACTTAGCTGGATTGATCTGAAGCTATGGGACTGTTGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003596 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATGCTTCACACTT
GAAAGCTTTTATTTTGAAAAATAAAATACAAAAGTCATTAGGTTGTAAATGTATAATGGT
AATTTCCCAACTTCTCAGAAACAATACAATGAATTAAATAATTAGGATATTTAATATTAT
TCACATCAATACACTTAAACAGGCACACGTGTTCTACAAAACCTTAGAACATGTACAGAC
AACCTATTTCAAAAGGAGACTGCCCAAGTCTGCTTACAGGATCCCATTTTTAAACCCTCT
CCTTGAAAACAAACGTCATAATGTAAGAAAAGAGAACAAGAGGAGTTTTAACAGGAAACA
CATGTTCCTTCTGGGCTCCTTACTGCAGGGCTGTGCATGAAATCGGGATCAAGAGCTCTT
TGCTCAGGAGGCAGATCCCTCACGAGGCCCAAAGCTGTGCGGACACCTCCATCCTCTCAG
TGGGAGGAAACTGGCAAATGCACAGGCGGCGCAGCCAAGGTGCAGACGCTCCACCAAGCT
TGGCAGGGGACAGCCAATCCTAGGAATTTCCCTTCTGCTGAAAAGGCAGCAATCTTTCCT
CATGTATGGAAGAGGCTCCTGGTTCTGCTACTCCACTTGCTCAGTCTGTGGTTTTTCTTT
AAGAAAGTCAGGTAGTTGGAATTCTCCCTTATAGACCCTTCGAGTGTTTTCAATAATTTT
GGGATCAGGTTTTCCGTAGTTAGGTGGGGTGGCATATGGGTCATATCCAAGCTTGGCAAG
CATANGAGCAATCACTGCCATGTCTTTGTAAACATCTGGCGGTATCTTCCCACCCCATTT
GATAGAGCTCCTACATTGACTGGCNNTGATACTGGNCTGNAGATCTCTNCACTTTTGACA
GAGAACTNCCCCAGCTTTCCCATNATNTCTTCATG
Restriction Sites NotI-NotI |
ACCN NM_003596
Insert Size 2110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003596.2, NP_003587.1
RefSeq Size 2033 bp
RefSeq ORF 1113 bp
Locus ID 8460
UniProt ID O60507
Cytogenetics 7q11.21
Domains Sulfotransfer
Summary Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003596" in other vectors (6)
SKU Description Size Price
RC202037 TPST1 (Myc-DDK-tagged)-Human tyrosylprotein sulfotransferase 1 (TPST1) 10 ug
$457.00
RC202037L1 Lenti ORF clone of Human tyrosylprotein sulfotransferase 1 (TPST1), Myc-DDK-tagged 10 ug
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RC202037L2 Lenti ORF clone of Human tyrosylprotein sulfotransferase 1 (TPST1), mGFP tagged 10 ug
$757.00
RC202037L3 Lenti ORF clone of Human tyrosylprotein sulfotransferase 1 (TPST1), Myc-DDK-tagged 10 ug
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RC202037L4 Lenti ORF clone of Human tyrosylprotein sulfotransferase 1 (TPST1), mGFP tagged 10 ug
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RG202037 TPST1 (tGFP-tagged) - Human tyrosylprotein sulfotransferase 1 (TPST1) 10 ug
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file-alt Citations

Fluorescent peptide sensors for tyrosylprotein sulfotransferase activity
Zhou, Duckworth, Geraghty
Anal. Biochem. (2014) 461C, 1-6 DOI: 10.1016/j.ab.2014.05.025
Cited by 12
AI Summary: OriGene contributed to this study by providing the cDNAs for the human tyrosylprotein sulfotransferases (hTPST1 and hTPST2), which were essential for expressing and studying the enzymes' activity in the fluorescent peptide-based assay.
Snippet: Materials. All peptides were synthesized by PEPTIDE 2.0 except PSGL-1 peptide (sequence: Biotin-RDRRQATEYEYLDYDFLPETEPPP) that was synthesized by the University of Minnesota Genomics Center. The cDNAs for the hTPSTs were purchased from Origene ... Related articles Cite SaveSaving
Showing 1-1 of 1 papers.
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