TIMELESS (NM_003920) Human Untagged Clone

SKU SC117684

TIMELESS (untagged)-Human timeless homolog (Drosophila) (TIMELESS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIMELESS
• Synonyms: hTIM; TIM; TIM1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003920, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGACTTGCACATGATGAACTGTGAACTTCTAGCCACATGTAGTGCCCTTGGGTACTTGGAGGGAGACA; CTTACCATAAGGAACCAGATTGCTTAGAGAGCGTGAAGGATCTGATCCGCTATTTGAGGCATGAGGATGA; GACACGAGATGTGCGGCAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACC; CAGCACCACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCAGCCT; TGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAGGTGCTAACTTATTT; GCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTCCTCAGTGAAACCTTGTATGAGCTG; CTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGACAACTTGCTGATTGAACGGATCCTACTGCTGGTCA; GAAATATTCTCCATGTCCCAGCTGACCTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGA; CCAGCTCCTCTGGGCGATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCT; GAGGAGCAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAACCCCGAGCAGC; TGGCGGGAGTAGGGCAGGGACGCTTAGCTCAGGAGCGGAGTGCAGATTTTGCAGAACTGGAGGTGTTGCG; CCAGCGAGAGATGGCAGAAAAGAAGACTCGAGCCCTCCAGCGAGGCAACAGGCATTCTCGATTTGGGGGC; TCCTATATTGTCCAGGGGTTGAAATCCATTGGGGAGAGGGACCTCATCTTTCACAAAGGCCTTCACAACC; TACGAAACTACAGTTCAGATTTGGGAAAGCAGCCGAAAAAGGTGCCTAAACGTCGCCAGGCCGCCCGAGA; GCTGTCCATTCAGCGCCGTTCTGCCCTCAATGTGAGGCTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG; GAGAACTGTTACAACCGGCTCATGGGATCAGTAAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAGCATG; ATGAGACCTATTATATGTGGGCCTTGGCTTTCTTCATGGCCTTCAACCGAGCTGCCTCCTTCCGGCCAGG; CCTGGTTTCTGAGACCCTCAGTGTCCGTACCTTCCACTTCATTGAGCAGAACCTCACCAACTACTATGAG; ATGATGCTGACTGACCGCAAGGAAGCTGCCTCCTGGGCACGCCGGATGCACTTGGCTCTGAAGGCCTATC; AGGAGCTGCTGGCAACAGTGAATGAGATGGACATATCTCCAGATGAGGCTGTGAGGGAGAGCAGCCGCAT; CATCAAGAACAATATTTTCTATGTGATGGAGTACCGAGAACTATTCCTGGCACTTTTTCGAAAGTTTGAT; GAGAGATGCCAGCCCCGCTCTTTCCTTCGTGACCTGGTGGAGACCACCCACCTCTTCCTCAAAATGTTGG; AGCGATTCTGTCGGAGCCGTGGGAACCTGGTGGTGCAGAACAAACAAAAGAAGAGAAGGAAGAAGAAGAA; GAAGGTCCTAGACCAGGCCATTGTTTCTGGTAATGTCCCATCTAGCCCAGAAGAAGTGGAGGCTGTGTGG; CCAGCCCTGGCTGAGCAGCTACAGTGCTGTGCCCAGAATTCTGAGCTCAGCATGGACTCCGTGGTTCCCT; TTGATGCGGCCTCAGAGGTGCCAGTGGAGGAGCAGCGGGCAGAAGCTATGGTACGGATCCAAGACTGTCT; CCTGGCTGGCCAGGCCCCACAGGCCCTGACTCTCCTGAGGTCTGCTCGGGAGGTGTGGCCTGAAGGAGAT; GTGTTTGGCTCTCAAGACATTTCTCCAGAGGAAGAGATCCAGTTGCTGAAACAAATCCTCTCTGCTCCAC; TTCCCCGGCAGCAGGGCCCAGAGGAACGTGGGGCAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGA; GGAGTTGCAAGTGGTCCAGGTGTCGGAGAAAGAATTTAATTTTCTGGACTACCTGAAACGCTTTGCATGT; TCAACTGTCGTTCGAGCCTATGTGCTGCTACTAAGGAGCTACCAGCAGAATAGTGCCCACACTAACCATT; GCATTGTGAAGATGCTGCACCGGCTGGCCCATGACCTCAAAATGGAAGCCCTACTTTTTCAGCTGTCAGT; CTTCTGCCTCTTCAATCGTCTGCTTAGTGACCCTGCTGCTGGAGCCTACAAAGAGCTAGTGACTTTTGCC; AAATACATCCTGGGCAAATTTTTTGCACTGGCTGCAGTCAACCAAAAAGCCTTTGTGGAGCTGTTGTTCT; GGAAGAACACAGCTGTGGTTCGAGAGATGACTGAGGGCTATGGCTCCCTGGATGACAGGTCTTCCAGTCG; CAGAGCACCTACATGGAGCCCCGAAGAAGAGGCTCATCTTCGGGAGCTGTACCTCGCCAATAAGGACGTG; GAAGGGCAGGATGTGGTGGAAGCCATCTTGGCCCACCTGAATACTGTTCCTCGAACACGCAAGCAGATCA; TCCACCATCTGGTACAGATGGGACTGGCTGACAGTGTCAAGGACTTCCAAAGGAAAGGAACCCATATTGT; ACTGTGGACGGGGGATCAGGAGTTGGAGCTGCAGCGGCTTTTTGAGGAATTCCGGGACTCAGATGATGTC; CTGGGTCATATCATGAAGAATATCACAGCCAAACGCTCACGGGCCCGAATAGTGGATAAACTCTTGGCTC; TGGGGCTGGTGGCTGAGCGGCGGGAGCTGTACAAGAAACGGCAGAAAAAGTTGGCATCCTCCATCTTGCC; AAATGGAGCGGAGTCCCTGAAAGATTTTTGCCAGGAAGATCTGGAAGAAGAGGAAAACCTGCCTGAGGAA; GACAGCGAAGAGGAAGAAGAAGGGGGCTCAGAAGCAGAACAAGTCCAGGGTAGCTTAGTCCTTTCAAATG; AAAACCTTGGTCAAAGCCTGCATCAGGAAGGCTTTTCTATCCCGCTCCTATGGCTCCAGAACTGCCTGAT; CCGAGCAGCTGATGATCGGGAAGAGGATGGCTGCTCCCAGGCCGTTCCATTGGTGCCACTCACAGAGGAA; AATGAGGAAGCCATGGAAAACGAACAGTTTCAGCAGCTGTTGCGCAAGCTAGGGGTTCGGCCCCCTGCCT; CTGGGCAGGAAACCTTCTGGCGAATTCCAGCCAAGCTGAGTCCTACCCAGCTCCGGAGGGCAGCAGCTTC; TTTGAGTCAACCAGAGGAGGAACAGAAGCTGCAGCCAGAGCTGCAGCCTAAAGTCCCTGGAGAGCAAGGC; TCTGATGAGGAGCACTGTAAAGAGCACCGAGCACAAGCCCTGAGGGCCCTCTTGCTAGCCCACAAGAAGA; AAGCGGGCCTGGCATCCCCAGAGGAGGAAGACGCTGTTGGTAAAGAGCCGCTGAAGGCAGCACCCAAGAA; ACGACAATTGCTGGACAGCGACGAGGAACAGGAAGAAGATGAGGGCAGGAACAGAGCACCAGAGTTGGGA; GCTCCAGGAATCCAAAAGAAGAAACGATACCAGATTGAGGATGATGAGGATGACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003920 unedited; GTATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGCTCCTCCGGGC; TTCTGAGGACCCGCAGGGAGCGGGCCTCATCATTTCTGTCTCCTGTTTCTCCACTTCCTT; CTCTATTGGTTGGTCCACTGATGTATGGACTTGCACATGATGAACTGTGAACTTCTAGCC; ACATGTAGTGCCCTTGGGTACTTGGAGGGAGACACTTACCATAAGGAACCAGATTGCTTA; GAGAGCGTGAAGGATCTGATCCGCTATTTGAGGCATGAGGATGAGACACGAGATGTGCGG; CAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACCCAGCAC; CACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCA; GCCTTGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAG; GTGCTAACTTATTTGCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTC; CTCAGTGAAACCTTGTATGAGCTGCTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGAC; AACTTGCTGATTGAACGGATCCTACTGCTGGTCAGAAATATTCTCCATGTCCCAGCTGAC; CTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGACCAGCTCCTCTGGGCG; ATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCTGAGGAG; CAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAAACCCGAG; CAGCTGTCGGAAGTAGGCCAGGNACGCTTTACTCAGGAGCGNAGTGCAGATTTTGCAGAA; CTGTNAGTATTGCCCCCCCGAGAGATGGCAGAAAGCAGATCGAGCCTTCCAGCGAGCCAC; AGGCATTCTCGATTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003920 unedited; AAGCTTGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTGAAAACTTCTAATCTTACATGTTTAATACCAAACACAAAGCAAAC; ACATTCATCATTTAAAGTACTATTTTCCAACCCTATTTCAAATGCAGGAATGGGCCTTTA; TTTGGTTTTTAAAGGATGATCATAGGAAACAAAATGAGGACAGAGGCATTTGGTATTTTT; TAAAAGGGGTACAAAACTCCCGCCTGTCATGTCATAAAGCTCCCCATTTGGGGCTGGGAC; CCACCTCCAAAAAAAAGCCCAAGGAACAGACCAATAAAAGGGGTCCGGGGGGCTTTCTCT; ATTTCACTCACTCCTCTTATTTGCTAAGGAGCTCCAATAACCAAAAAAAATGGTTCCTAA; AAAAAAAAAACTAAATCTCCAAAAAGCTGCTGGGGCATACCGATAAAAACTGGGAGAAAC; AAAGACTGACAGCAGAAAAGTCCAATACTGCTGCTGAAGTTTCTCAACCTAAATCCGTGA; AAGAACCTGGGATTCTACTGCTCTGTCCAATAAGAGGAGCTTCTGGGTCCTGTATGACCC; TTCCAACTCTGACTTGAATGCACCATCTAAAAACGTGTCTATCTCTACCCCTAAGCTTCT; TTAACTCTTCAGGCATCCTCATCATCCTCAATCTGGGATCCGTTCTTCTTTTGGATTCCT; GGAGCTCCAACTCTGGGGCTCTGTTCCTGCCCTATATTCTTCCTGTNCCTCGNGCGTGGC; AACAATTGTCGTTTCTGGGTGCTGGCTTCAAGGCTTTTTACCAAAGCGTTTTCTTCTTTT; GGGAATGCAAGCCCGTTTTTTTTTTGGGGCCTACAAAAGGCCTCAAGGCTTGTGCTGGGG; GCTTTTAAGGGCTCCTCATAAACCTTGCTTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003920
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003920.2, NP_003911.1
• RefSeq Size: 4409 bp
• RefSeq ORF: 3627 bp
• Locus ID: 8914
• UniProt ID: Q9UNS1
• Cytogenetics: 12q13.3
• Domains: TIMELESS, TIMELESS_C
• Protein Families: Transcription Factors
• Summary: The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).