SH2D2A (NM_003975) Human Untagged Clone

SKU SC117636

SH2D2A (untagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SH2D2A
• Synonyms: F2771; SCAP; TSAD; VRAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117636 sequence for NM_003975 edited (data generated by NextGen Sequencing); ATGGAGTTCCCCCTGGCCCAGATATGTCCCCAAGGGAGTCACGAAGCCCCCATCCCAACC; TTCAGCACCTTCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACT; GCGGCATCTCCCCAGGCCCCGGAGGCTGCCTCCAGCACAGGGAATGCTGAGAGGGCAGAG; GAGGTGCCTGGAGAAGGAAGCCTGTTCCTGCAGGCCGAGACCCGGGCTTGGTTCCAGAAG; ACCCAGGCCCACTGGCTCCTGCAGCACGGGGCAGCCCCTGCCTGGTTCCATGGCTTCATC; ACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCAAGCCTCAGGGGTGCTACTTGGTGCGG; TTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCGGACTTGCTGCCGCCAC; TTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGCGCCCAC; GCGCGGCTGCAGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAG; ACGCTCACCGAGCCCCTCGCCCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACC; GAAGAATCAAACTTTGGAAGCAAAAGCCAGGACCCAAACCCCCAGTACAGCCCAATCATC; AAACAGGGGCAAGCCCCAGTCCCGATGCAGAAAGAGGGGGCCGGGGAGAAGGAGCCCTCC; CAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCAAACCTCAGCTGCCCCCAGAAGTCTAC; ACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCCCTCCAATCCTATCTAC; AATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGGGAAGCC; CCCAGCAACATCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCT; GTCCTACGGAAGAGCTGGTCCAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAG; CTGCATTCTGAGAACTCTGTGATTGGGCAAGGCCCTCCCCTGCCCCACCAGCCCCCACCC; GCCTGGAGACACACCCTCCCCCACAATCTTTCTAGACAGGTGCTTCAGGACAGAGGACAG; GCATGGCTTCCCCTTGGGCCTCCTCAGTAG; ; Clone variation with respect to NM_003975.3; 155 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003975 unedited; TTCAGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGNAGAGAT; GATGAGTTGCTGAGTGTGCACACCTTTCCGGAACACATACACACACCCTGCTCTGGGATC; CCTTGTGAGGCTGCCCTCATGGAGTTCCCCCTGGCCCAGATATGTCCCCAAGGGAGTCAC; GAAGCCCCCATCCCAACCTTCAGCACCTTCCAGATCACAGACATGACCCGCAGGAGCTGC; CAGAACCTGGGCTACACTGCGGCATCTCCCCAGGCCCCGGAGGCTGCCTCCAGCACAGGG; AATGCTGAGAGGGCAGAGGAGGTGCCTGGAGAAGGAAGCCTGTTCCTGCAGGCCGAGACC; CGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAGCACGGGGCAGCCCCTGCC; TGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCAAGCCTCAG; GGGTGCTACTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGC; CGGACTTGCTGCCGCCACTTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTG; GGCGAGGACAGCGCCCACGCGCGGCTGCAGGACCTGCTGCTGCACTACACCGCGCACCCG; CTCAGCCCCTACGGGGAGACGCTCACCGAGCCCCTCGCCCGACAGACTCCTGAGCCTGCA; GGACTTTCCCTGAGGACCGAAGAATCAAACTTTGGAAGCANAAGCCAGGACCCAAACCCC; CAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCGATGCANAAAAGAGGGGCC; GGNNGAGAAGAGCCCTCCAGCTGCTCAGGCCCAAGCCTCCATCCCCGCCAAACTCAGCTG; CCCCCAGAAGTCTACACATCCCTGGTTCACGACACCGNCCGGCCCCACGCCCCAAGCCCT; NCAATNCTATCTACAATGAGCCCTGATGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003975 unedited; NGGTCTTCTATGAACGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTCCCTGGAGAAAAAGCAGTTGGGGATGATTTATTACCCAGACAAGAATCAAC; AAACAAGTTCTAACATCTGTTCTCACCAAACCATTCCTCAAACAACAGCAGCATTGGACC; AGGGCCCAAACTCAAAAGGCTCAAAGGGCCAAGGAAATTAGACTCCAGGAGGAAAAACCA; GGACAGTGGGAGCTAACAGGGCCCTCCTTGCACACTTCTTTGCTGTCTTAAATCACCAAG; GACTGAGCCATTGGATTCTTGGGATCTTCTTAGGAGGGAGCAGGCTCCGCATTTGCTTCT; CTGACCTCCAGGCTTCTTTGTTGGGGGTCAGGCCAGACCGCCTACTGAGGAGGCCCAAGG; GGAAGCCATGCCTGTCCTCTGTCCTGAAGCACCTGTCTAGAAAGATTGTGGGGGAGGGTG; TGTCTCCAGGCGGGTGGGGGCTGGTGGGGCAAGGGAGGGCCTTGCCCAATCACAGAGTTC; TCAGAATGCAGCTGGGAGCCACCTGTATTCTGGCCTCCTGGGACAGGCCTGGACCAGCTC; TTCCGTAAGAAGGGTGCCCAAAGGTGGCGGGTAGGCCCTCATCTTCCACTTCCACATAAA; TGTTGCTGGGGGGCTTCCCCAGGGCTGCCCCGGCCCATGGCATAAAAAGCTATGGGTTCA; TCAAGCTCATTGTAGATAGGATTGGAAGGCTTGGGGCCGGGGGCCCGGCGGTTGTTTGGA; ACAGGATTGGGTACACTTCTGGGGGCAACTGAAGGTTGGCGGGGAATGGAAGGCTTGGGC; CCGAACCAACGGAGAAGGCCCCTTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003975
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003975.2, NP_003966.1
• RefSeq Size: 1651 bp
• RefSeq ORF: 1170 bp
• Locus ID: 9047
• UniProt ID: Q9NP31
• Cytogenetics: 1q23.1
• Domains: SH2
• Protein Pathways: VEGF signaling pathway
• Summary: This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 5 encode the same isoform (2).