CA8 (NM_004056) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CA8 |
| Synonyms | CA-RP; CA-VIII; CALS; CAMRQ3; CARP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004056 |
| Insert Size | 2870 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004056.4, NP_004047.3 |
| RefSeq Size | 2278 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 767 |
| UniProt ID | P35219 |
| Cytogenetics | 8q12.1 |
| Domains | carb_anhydrase |
| Protein Families | Druggable Genome |
| Protein Pathways | Nitrogen metabolism |
| Summary | The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2016 Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
"NM_004056" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210228 | CA8 (Myc-DDK-tagged)-Human carbonic anhydrase VIII (CA8) | 10 ug |
$300.00
|
|
| RC210228L1 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210228L2 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), mGFP tagged | 10 ug |
$600.00
|
|
| RC210228L3 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210228L4 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), mGFP tagged | 10 ug |
$600.00
|
|
| RG210228 | CA8 (tGFP-tagged) - Human carbonic anhydrase VIII (CA8) | 10 ug |
$500.00
|
|
Citations
Carbonic Anhydrase 8 Expression in Purkinje Cells Is Controlled by PKCγ Activity and Regulates Purkinje Cell Dendritic Growth
Shimobayashi, Wagner, Kapfhammer
Mol. Neurobiol. (2015)
Shimobayashi, Wagner, Kapfhammer
Mol. Neurobiol. (2015)
Cited by 30
AI Summary: OriGene products, specifically the CMV-CA8 and CMV-PKCγ plasmids, were used in this study to overexpress CA8 and PKCγ in Purkinje cells, enabling the researchers to investigate the roles of these proteins in regulating dendritic growth.
Snippet: activity. Materials and Methods Plasmid Construction Plasmid L7-green fluorescent protein (GFP) was previously described [17]. CMV-CA8 and CMV-PKCγ plasmids were from Origene (Rockville, MD, USA). Restriction sites ... Cite SaveSaving
Showing 1-1 of 1 papers.
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