BCL2A1 (NM_004049) Human Untagged Clone

SKU SC117596

BCL2A1 (untagged)-Human BCL2-related protein A1 (BCL2A1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BCL2A1
• Synonyms: ACC-1; ACC-2; ACC1; ACC2; BCL2L5; BFL1; GRS; HBPA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004049 edited; GAATTCGGCACCAGCTTCAAGGTGAGCCAGCTCAAGACTTTGCTCTCCACCAGGCAGAAG; ATGACAGACTGTGAATTTGGATATATTTACAGGCTGGCTCAGGACTATCTGCAGTACGTC; CTACAGATACCACAACCTGGATCAGGTCCAAGCAAAACGTCCAGAGTGCTACAAAAGGTT; GCGTTCTCAGTCCAAAAAGAAGTGGAAAAGAATCTGAAGTCATGCTTGGACAATGTTAAT; GTTGTGTCCGTAGACACTGCCAGAACACTATTCAACCAAGTGATGGAAAAGGAGTTTGAA; GACGACATCATTAACTGGGGAAGAATTGTAACCATATTTGCATTTGAAGGTATTCTCATC; AAGAAACTTCTACGACAGCAAATTGCCCCGGATGTGGATACCTATAAGGAGATTTCATAT; TTTGTTGCGGAGTTCATAATGAATAACACAGGAGAATGGATAAGGCAAAACGGAGGCTGG; GAAAATGGCTTTGTAAAGAAGTTTGAACCTAAATCTGGCTGGATGACTTTTCTAGAAGTT; ACAGGAAAGATCTGTGAAATGCTATCTCTCCTGAAGCAATACTGTTGACCAGAAAGGACA; CTCCATATTGTGAAACCGGCCTAATTTTTCTGACTGATATGGAAACGATTGCCAACACAT; ACTTCTACTTTTAAATAAACAACTTTGATGATGTAACTTGACCTTCCAGAGTTATGGAAA; TTTTGTCCCCATGTAATGAATAAATTGTATGTATTTTTCTCTAAAAAAAAAAAAAAAAAA; AACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004049 unedited; CAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCGCTTCAAGTGAGCCA; GCTCAAGACTTTGCTCTCCACCAGGCAGAAGATGACAGACTGTGAATTTGGATATATTTA; CAGGCTGGCTCAGGACTATCTGCAGTACGTCCTACAGATACCACAACCTGGATCAGGTCC; AAGCAAAACGTCCAGAGTGCTACAAAAGGTTGCGTTCTCAGTCCAAAAAGAAGTGGAAAA; GAATCTGAAGTCATGCTTGGACAATGTTAATGTTGTGTCCGTAGACACTGCCAGAACACT; ATTCAACCAAGTGATGGAAAAGGAGTTTGAAGACGACATCATTAACTGGGGAAGAATTGT; AACCATATTTGCATTTGAAGGTATTCTCATCAAGAAACTTCTACGACAGCAAATTGCCCC; GGATGTGGATACCTATAAGGAGATTTCATATTTTGTTGCGGAGTTCATAATGAATAACAC; AGGAGAATGGATAAGGCAAAACGGAGGCTGGGAAAATGGCTTTGTAAAGAAGTTTGAACC; TAAATCTGGCTGGATGACTTTTCTAGAAGTTACAGGAAAGATCTGTGAAATGCTATCTCT; CCTGAAGCAATACTGTTGACCAGAAAGGACACTCCATATTGTGAAACCGGCCTAATTTTT; CTGACTGATATGGAAACGATTGCCAACACATACTTCTACTTTTAAATAAACACTTTGATG; ATGTACTTGACCTTCAGAGTATGGANATTTGTCCCATGTATGAATAATTGTATGTATTTT; TCTCTAAAAAAAAAAAAAAACTCACTCTAGATGCGGNCGCGCATAGCTGTTCTGAACAAN; CCGGNTGGATCCTGGACCTNCCAGGCCTTCTGCCTTGAGTGCCATCATGCACAGCTGCTA; TAAATATGTCATGTGCAGCTATGTNGGGGGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004049 unedited; NGGGGCNANNNCNNNNNCNNNTNTTCNNNNAANGNTTTATTTTAGGCCCGCGGNCCGCAT; ACTAGGATCGAGTTTTTTTTTTTTTTTTTTTNTAGANAAAATACATACAATTTATTCATT; ACATGGGGACAAAATTTCCATAACTCTGGAAGGTCAAGTTACATCATCAAAGTTGTTTAT; TTAAAAGTAGAAGTATGTGTTGGCAATCGTTTCCATATCAGTCAGAAAAATTAGGCCGGT; TTCACAATATGGAGTGTCCTTTCTGGTCAACAGTATTGCTTCAGGAGAGATAGCATTTCA; CAGATCTTTCCTGTAACTTCTAGAAAAGTCATCCAGCCAGATTTAGGTTCAAACTTCTTT; ACAAAGCCATTTTCCCAGCCTCCGTTTTGCCTTATCCATTCTCCTGTGTTATTCATTATG; AACTCCGCAACAAAATATGAAATCTCCTTATAGGTATCCACATCCGGGGCAATTTGCTGT; CGTAGAAGTTTCTTGATGAGAATACCTTCAAATGCAAATATGGTTACAATTCTTCCCCAG; TTAATGATGTCGTCTTCAAACTCCTTTTCCATCACTTGGTTGAATAGTNGTCTGGCAGTG; TCTACGGACACAACATTAACATTTGTCCAGCATGACTTCAGATTCTTTTCCACTTTCTTT; TTGGACTGAGAACGCACCCTTTTGTAGCACTCTGGACGTTNTGCTTGGACCTGATCCAGG; TTGTGGTATCTGTAGGACGTACTGCAGATAGTCCTTGACCAGCCTGTAATATATCCCAAT; TCACAGTCTGTCATCTTCTGCCTGGTGGAAAGCAAAAGCCTTGAGCTGGCTCACCTTGAA; GCTGGTGCCGAATTCCCGGCCGCCTTATGGAGTCGTATAACAAATTTGACGGTTCACTAA; CGACTTTGCTATTAGGACTTCCACCGACACGCTACCGGCATTTGCGTAACGGGCCGGGTA; TTACCAATTTTGGAAAGTCCGTTGATTTGGGCCCAACCACTCCCTTGACGTAAGGGGGGG; GG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004049
• Insert Size: 850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004049.2, NP_004040.1
• RefSeq Size: 885 bp
• RefSeq ORF: 528 bp
• Locus ID: 597
• UniProt ID: Q16548
• Cytogenetics: 15q25.1
• Domains: Bcl-2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways
• Summary: This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).