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CD83 (NM_004233) Human Untagged Clone

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SKU
SC117490
CD83 (untagged)-Human CD83 molecule (CD83), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CD83
Synonyms BL11; HB15
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_004233 edited
ATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCCCGCGACG
CCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCCCTGGGAT
CCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGAGAGGATG
GAGACACCCCAGGAAGACCACCTCAGAGGACAGCACTATCATCAGAAGGGGCAAAATGGT
TCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTACCAGCTGC
AACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCTAAGTGGC
AAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTTTAAGAAA
TACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACTCATCATT
TTCACTTGTAAGTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGCTGGCATG
GAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGACTCCTCAC
AAGACAGAACTGGTATGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004233 unedited
NGGGTCGCATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACCAGGCGCAGCGC
TCCAGCCATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCC
CGCGACGCCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCC
CTGGGATCCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGA
GAGGATGGAGACACCCCAGGAAGACCACCTCAGAGGACAGCACTATCATCAGAAGGGGCA
AAATGGTTCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTAC
CAGCTGCAACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCT
AAGTGGCAAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTT
TAAGAAATACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACT
CATCATTTTCACTTGTAAGTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGC
TGGCATGGAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGAC
TCCTCACAAGACAGAACTGGTATGAGCAGGATTTCTGCAGGTTCTTCTTCCTGAAGCTGA
GGCTCAGGGGTGTGCCTGTCTGTTACACTGGAGGAGAGAAGAATGAGCCTACGCTGAAGA
TGGCATCCTGTGAAGTCCTTCACCTCACTGAAAACATCTGAAGGGGATCCCACCCCATTT
TCTGTGGGCAGGCCTCGAAACCATCCATGACCCATAGCATGAGCCCTGCTGCTTCTCATG
GCCCCTTTTCAGCGTGTATGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004233 unedited
NCATCTGNACCCGGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTTTTAACATGCA
TACGTTTAATAACATGAAAGCCAAAACAGAGGTAAAAACACTAAATCAACTTGGTATCCG
TTTTACTCCCTAATATTAGGGGGACAGTGAACATATAACTCAAGCAATAAAATGCCTTTG
GAAACCAAGAAGGGAAGGGCCCAAGTTTGATAAGGGAAAGCCAACAATGGCTAAAACATC
CATGCAACACTTCGTGAAGTCCCTTCTGCCAGCGGCTCCCGTGTGTCCATATCTTGGAGT
GTGTTAGGCTACTGCTTCAAGAACAGACCGTGGACCTGGCTTCCTCACTTCCCCAAAAGA
ATTCCTACAGAAGGCTACAGGAAAGGCTTCTTGGTAACCTTCTTTGTTTCAAACAATGGA
AAACATGAGAAATGGATAGCACCCTCTCATCCCCAAAACACCATCATCATAGCAAGGATA
ATGACTCAATGGAGTTTCGAATCTGTATAGTGCTTTATATGTTTTCAAATAATTTTCACG
TATATGATCTTCATTAGCCCATGCAACAGCCTTGTGGTTTACAATGGAGAAAGCAGACCC
CAGAAGGAACTTGCTTAAGATCATGCACCCAGATAGGGGGACAAGTGTGCGAGCACCCAG
ACTGCACCCGTAGCCCAACTTTTCCAGCCCGGCCATAAAATAACTCTCTATCTTGACACC
CTCTCTGACTTTTTTTCCCTTTTTATGTCACGGCTGCCTGTTAGTCAACAATGCTTTTCC
ACTTCCGATACCGATTTCACAAGCAGAAAAACCCAAACCTTTTTTTGCCAGTGCCTCTCG
GATATATAAATAAATGGGGGCCCTCTCTTTTCTGGGTTCTTCTAGGGAGGCCTCTCACAC
AGAAAAAAACCCGCTCGGGGAATACCCATAGACCCCGTCTTCCT
Restriction Sites NotI-NotI |
ACCN NM_004233
Insert Size 2220 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004233.2, NP_004224.1
RefSeq Size 2574 bp
RefSeq ORF 618 bp
Locus ID 9308
UniProt ID Q01151
Cytogenetics 6p23
Domains IG
Protein Families Transmembrane
Summary The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
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Documents
Resources
"NM_004233" in other vectors (6)
SKU Description Size Price
RC205302 CD83 (Myc-DDK-tagged)-Human CD83 molecule (CD83), transcript variant 1 10 ug
$300.00
RC205302L1 Lenti ORF clone of Human CD83 molecule (CD83), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC205302L2 Lenti ORF clone of Human CD83 molecule (CD83), transcript variant 1, mGFP tagged 10 ug
$600.00
RC205302L3 Lenti ORF clone of Human CD83 molecule (CD83), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC205302L4 Lenti ORF clone of Human CD83 molecule (CD83), transcript variant 1, mGFP tagged 10 ug
$600.00
RG205302 CD83 (tGFP-tagged) - Human CD83 molecule (CD83), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

The Expression and Characterization of Functionally Active Soluble CD83 by Pichia pastoris Using High-Density Fermentation
Guo, Li, Song et al
PLOS ONE (2014) 9, e89624
AI Summary: OriGene’s pCMV6-XL4-CD83 human cDNA clone was used as the template to amplify the sCD83 gene for expression studies in this paper.
Snippet: Using the human cDNA clone pCMV6-XL4-CD83 (OriGene) as the template, the sCD83 gene was obtained by polymerase chain reaction (PCR) and inserted between the Xho I and EcoR I sites in pPIC9K and in-frame with the Kex2 cleavage site in the sequence of ... Cite SaveSaving
Showing 1-1 of 1 papers.
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