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S1PR2 (NM_004230) Human Untagged Clone

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SKU
SC117485
S1PR2 (untagged)-Human sphingosine-1-phosphate receptor 2 (S1PR2)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol S1PR2
Synonyms AGR16; DFNB68; EDG-5; EDG5; Gpcr13; H218; LPB2; S1P2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_004230, RT-PCR generated
ATGGGCAGCTTGTACTCGGAGTACCTGAACCCCAACAAGGTCCAGGAACACTATAATTAT
ACCAAGGAGACGCTGGAAACGCAGGAGACGACCTCTCGCCAGGTGGCCTCGGCCTTCATC
GTCATCCTCTGTTGCGCCATTGTGGTGGAAAACCTTCTGGTGCTCATTGCGGTGGCCCGA
AACAGCAAGTTCCACTCGGCAATGTACCTGTTTCTGGGCAACCTGGCCGCCTCCGATCTA
CTGGCAGGCGTGGCCTTCGTAGCCAATACCTTGCTCTCTGGCTCTGTCACGCTGAGGCTG
ACGCCTGTGCAGTGGTTTGCCCGGGAGGGCTCTGCCTTCATCACGCTCTCGGCCTCTGTC
TTCAGCCTCCTGGCCATCGCCATTGAGCGCCACGTGGCCATTGCCAAGGTCAAGCTGTAT
GGCAGCGACAAGAGCTGCCGCATGCTTCTGCTCATCGGGGCCTCGTGGCTCATCTCGCTG
GTCCTCGGTGGCCTGCCCATCCTTGGCTGGAACTGCCTGGGCCACCTCGAGGCCTGCTCC
ACTGTCCTGCCTCTCTACGCCAAGCATTATGTGCTGTGCGTGGTGACCATCTTCTCCATC
ATCCTGTTGGCCATCGTGGCCCTGTACGTGCGCATCTACTGCGTGGTCCGCTCAAGCCAC
GCTGACATGGCCGCCCCGCAGACGCTAGCCCTGCTCAAGACGGTCACCATCGTGCTAGGC
GTCTTTATCGTCTGCTGGCTGCCCGCCTTCAGCATCCTCCTTCTGGACTATGCCTGTCCC
GTCCACTCCTGCCCGATCCTCTACAAAGCCCACTACTTTTTCGCCGTCTCCACCCTGAAT
TCCCTGCTCAACCCCGTCATCTACACGTGGCGCAGCCGGGACCTGCGGCGGGAGGTGCTT
CGGCCGCTGCAGTGCTGGCGGCCGGGGGTGGGGGTGCAAGGACGGAGGCGGGGCGGGACC
CCGGGCCACCACCTCCTGCCACTCCGCAGCTCCAGCTCCCTGGAGAGGGGCATGCACATG
CCCACGTCACCCACGTTTCTGGAGGGCAACACGGTGGTCTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004230 unedited
GGGGCGCTAACTTGAGTTTTGTTAACACGTACTTACTATAGGCGGCCGCGCATTCAGATC
TGGTACCGAGCTCGGTTCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTATGGG
CAGCTTGTACTCGGAGTACCTGAACCCCAACAAGGTCCAGGAACACTATAATTATACCAA
GGAGACGCTGGAAACGCAGGAGACGACCTCTCGCCAGGTGGCCTCGGCCTTCATCGTCAT
CCTCTGTTGCGCCATTGTGGTGGAAAACCTTCTGGTGCTCATTGCGGTGGCCCGAAACAG
CAAGTTCCACTCGGCAATGTACCTGTTTCTGGGCAACCTGGCCGCCTCCGATCTACTGGC
AGGCGTGGCCTTCGTAGCCAATACCTTGCTCTCTGGCTCTGTCACGCTGAGGCTGACGCC
TGTGCAGTGGTTTGCCCGGGAGGGCTCTGCCTTCATCACGCTCTCGGCCTCTGTCTTCAG
CCTCCTGGCCATCGCCATTGAGCGCCACGTGGCCATTGCCAAGGTCAAGCTGTATGGCAG
CGACAAGAGCTGCCGCATGCTTCTGCTCATCGGGGCCTCGTGGCTCATCTCGCTGGTCCT
CGGTGGCCTGCCCATCCTTGGCTGGAACTGCCTGGGCCACCTCGAGGCCTGCTCCACTGT
CCTGCCTCTCTACGCCAAGCATTATGTGCTGTGCGTGGTGACCATCTTCTCCATCATCCT
GTTGGCCAACGTGGCCCTGTACGTGCGCATCTACTGCGTGGTCCGCTCAAGCCACGCTGA
CATGGCCGCCCCGCAGACGCTAGCCCTGCTCAAGAGGTCACCATCGTGCTAGGCGTCTTT
ATCGTCTGCTGGCTGCCCGCCTTCAGCATCCTCCTTTCTGACTATGCCTGTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004230 unedited
TANGAGCCTGGGGNAGGGTCACAGGGATGCCACCCGGGTTCTGTTCAGGAAACAGCTATG
ACCGCGGCCGCCAGTGTGATGGATATCTGCAGAATTTCAGACCACCGTGTTGCCCTCCAG
AAACGTGGGTGACGTGGGCATGTGCATGCCCCTCTCCAGGGAGCTGGAGCTGCGGAGTGG
CAGGAGGTGGTGGCCCGGGGTCCCGCCCCGCCTCCGTCCTTGCACCCCCACCCCCGGCCT
CCAGCACTGCAGCGGCCGAAGCACCTCCCGCCGCAGGTCCCGGCTGCGCCACGTGTAGAT
GACGGGGTTGAGCAGGGAATTCAGGGTGGAGACGGCGAAAAAGTAGTGGGCTTTGTAGAG
GATCGGGCAGGAGTGGACGGGACAGGCATAGTCCAGAAGGAGGATGCTGAAGGCGGGCAG
CCAGCAGACGATAAAGACGCCTAGCACGATGGTGACCGTCTTGAGCAGGGCTAGCGTCTG
CGGGGCGGCCATGTCAGCGTGGCTTGAGCGGACCACGCAGTAGATGCGCACGTACAGGGC
CACGATGGCCAACAGGATGATGGAGAAGATGGTCACCACGCACAGCACATAATGCTTGGC
GTANAGAGGCAGGACAGTGGAGCAGGCCTCGAGGTGGCCCAGGCAGTTCCAGCCAAGGAT
GGGCAGGCCACCGAGGACCAGCGAGATGAGCCACGAGGCCCCGATGAGCAGAAGCATGCG
GCAGCTCTTGTCGCTGCCATACAGCTTGACCTTGGCAATGGGCACGTGGCGCTCAATGGC
GATGGCCAGGAGGCTGAAGACAAGAGCCGAGAGCGTGATGAAGG
Restriction Sites Please inquire |
ACCN NM_004230
Insert Size 1000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004230.2, NP_004221.2
RefSeq Size 1154 bp
RefSeq ORF 1062 bp
Locus ID 9294
UniProt ID O95136
Cytogenetics 19p13.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Summary This gene encodes a member of the G protein-coupled receptors, as well as the EDG family of proteins. The encoded protein is a receptor for sphingosine 1-phosphate, which participates in cell proliferation, survival, and transcriptional activation. Defects in this gene have been associated with congenital profound deafness. provided by RefSeq, Mar 2016
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Documents
Resources
"NM_004230" in other vectors (6)
SKU Description Size Price
RC210163 S1PR2 (Myc-DDK-tagged)-Human sphingosine-1-phosphate receptor 2 (S1PR2) 10 ug
$457.00
RC210163L1 Lenti ORF clone of Human sphingosine-1-phosphate receptor 2 (S1PR2), Myc-DDK-tagged 10 ug
$757.00
RC210163L2 Lenti ORF clone of Human sphingosine-1-phosphate receptor 2 (S1PR2), mGFP tagged 10 ug
$757.00
RC210163L3 Lenti ORF clone of Human sphingosine-1-phosphate receptor 2 (S1PR2), Myc-DDK-tagged 10 ug
$757.00
RC210163L4 Lenti ORF clone of Human sphingosine-1-phosphate receptor 2 (S1PR2), mGFP tagged 10 ug
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RG210163 S1PR2 (tGFP-tagged) - Human sphingosine-1-phosphate receptor 2 (S1PR2) 10 ug
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file-alt Citations

The Sphingosine Kinase 1 / Sphingosine-1-Phosphate Pathway in Pulmonary Arterial Hypertension
Chen, Tang, Sysol et al
Am. J. Respir. Crit. Care Med. (2014)
AI Summary: In this study, the human S1PR2 expression plasmid (pCMV6-XL4 vector, Cat.# SC117485, OriGene Technologies) from OriGene was used to transfect pulmonary artery smooth muscle cells for functional analysis of the SphK1/S1P pathway in pulmonary arterial hypertension.
Snippet: hPASMCs were transfected with SphK1 WT and G82D dominant-negative plasmids, or with human S1PR2 in pCMV6-XL4 vector (Cat.#SC117485, OriGene Technologies) using XfectTM Transfection reagent (Cat.#631318, Clontech). Scrambled siRNA (D- ... Cite SaveSaving
Showing 1-1 of 1 papers.
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