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Caspase 3 (CASP3) (NM_004346) Human Untagged Clone

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SKU
SC117447
CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Caspase 3
Synonyms CPP32; CPP32B; SCA-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117447 sequence for NM_004346 edited (data generated by NextGen Sequencing)
ATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATC
ATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGAT
TATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGA
ATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAAC
TTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATG
CGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGC
CATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACA
AACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATT
CAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGAT
GACATGGCGTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCA
CCTGGTTATTATTCTTGGCGAAATTCAAAGGATGGCTCCTGGTTCATCCAGTCGCTTTGT
GCCATGCTGAAACAGTATGCCGACAAGCTTGAATTTATGCACATTCTTACCCGGGTTAAC
CGAAAGGTGGCAACAGAATTTGAGTCCTTTTCCTTTGACGCTACTTTTCATGCAAAGAAA
CAGATTCCATGTATTGTTTCCATGCTCACAAAAGAACTCTATTTTTATCACTAA

Clone variation with respect to NM_004346.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004346 unedited
ATATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGCGGCTCCT
AGCGGATGGGTGCTATTGTGAGGCGGTTGTGGAAGTTAATAAAGGTATCCATGNGAGAAC
ACTNGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATCATACATGG
AAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGATTATCCTGA
GATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGAATGACATC
TCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAACTTGAAATA
TGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATGCGTGATGT
TTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGCCATGGTGA
AGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACAAACTTTTT
CAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATTCAGGCCTG
CCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGATGACATGGC
GTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCACCTGGTTA
TTATTCTTGGCGAAATCANAGGATGGCTCCTGGTTCATCCAGTCGCTTTGTGCCATGCTG
AAACAGTATGCCGGACAGCTTGAAATTATGCACATTCTTACCCGGGTAACCGCAAGGTGG
GCACAGAATTTGAGTCCCTTTCCTTTGACGCTACTTTTCATGCAAAGAAACAGATTCCAT
GGATTGGTTCCATGCTCACCAAAGAACTCTATTTNTATCACTAAAGAAAAGGGTGGNTTG
GTGGGGTTTTTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004346 unedited
ACTATGAACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTAATTTTAAGTTTGAATGTATATTTTGGAAATAAAAATGGAAAA
TAACAAATTTGTACAAAATTGTCACATAAAAACACACTTTAAAATACCATTACATGCTGG
GGGTATTTACAAAAGTTACAGGTCTAGTAAAAAAAAAAAAACTGTCATTGACAAATGCGA
GCTCATTTTTTGAGCAACAAAAAATCTCCCGTGAAATGTCATACTGACAGCCAGTGAAAC
TTGGTGCAGTGACGGCTCCCCACCTGCTGAGGCCTGGAGCCTGCCTCCCGGGCTGAGTGC
TCAACTTTGGCCTCCGGCTGGATGCCGTCTAAAGTCCTATGGGCTCATAGGTCTTTTTTT
TTCAGTAAACAGTATAAAACATTACTATTTTCCTTTGATTGGAAACTTCAACAGAAAATA
ACATACTTAAAAATTTTGCTACAAAAAGTACTATGATTTTTATTGCCTCACCACCTTTAA
AACATTTCCACTTCACAAGTTTCTGATTTGCTTCACTTTCTTACTTGGCGATGGCGCCCT
GGCAGCATCATCCACACATACCAGTGCGTATGGAAAAATGGGCTGTAGGCCCTTCCTGCG
TGGTCCATGGCTCTGCCTTCATGGAACAGGCTGAGTCATGTCTCTGCTCAGGCTCAAACC
ATCTACTCTCAAGTTACAGGCAGCTTAGCCCATTCATTTATTGCTTTCCTTATATTCCAC
TGTTTATTATTATAAAGTTGGATTTCCATATGTTCCCTATAGAAATTATTCCACATTACC
CTGCCTAATAATCCAACAAGTTGGGGGGTTTTTATATAATAGCTCTCAACATTATTGCTG
GTTTTTGAAGCCTCACTGAATGCCTAGGGACAATCAAAGTATTTTTGCTTGGAGATCAGG
GCATTGTACC
Restriction Sites NotI-NotI |
ACCN NM_004346
Insert Size 2740 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004346.3, NP_004337.2
RefSeq Size 2689 bp
RefSeq ORF 834 bp
Locus ID 836
UniProt ID P42574
Cytogenetics 4q35.1
Domains CASc, ICE_p10, ICE_p20
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis
Summary The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. provided by RefSeq, Aug 2017
Transcript Variant: This variant (1, also known as alpha) encodes the longest isoform (a). Variants 1, 2, and 3 all encode the same isoform (a).
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Documents
Resources
"NM_004346" in other vectors (4)
SKU Description Size Price
RC223662 CASP3 (Myc-DDK-tagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha 10 ug
$300.00
RC223662L3 Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, Myc-DDK-tagged 10 ug
$600.00
RC223662L4 Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, mGFP tagged 10 ug
$600.00
RG223662 CASP3 (tGFP-tagged) - Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha 10 ug
$489.00 $500.00

file-alt Citations

Caspase-dependent cell death-associated release of nucleosome and damage-associated molecular patterns
Yoon, Park, Han et al
Cell Death Dis (2014) 5, e1494 DOI: 10.1038/cddis.2014.450
Cited by 27
AI Summary: OriGene products contributed to this study by providing cDNAs for caspase 3, CAD, EndoG, calpain 1, and calpain 2, which were used to investigate the molecular mechanisms underlying caspase-dependent regulated necrosis and the release of nucleosomes and DAMPs.
Snippet: cDNAs of caspase 3, CAD, EndoG, calpain 1 and calpain 2 were purchased from OriGene (Rockville, MD, USA), calpastatin cDNA was from Shi-Yong Sun (Emory University School of Medicine), being subcloned into pCDH-EF2-MCS-T2A-Puro, a lentiviral vector for ... Cite SaveSaving
Showing 1-1 of 1 papers.
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