Caspase 3 (CASP3) (NM_004346) Human Tagged ORF Clone
SKU
RC223662
CASP3 (Myc-DDK-tagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Caspase 3 |
| Synonyms | CPP32; CPP32B; SCA-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004346 |
| ORF Size | 831 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004346.4 |
| RefSeq Size | 2689 bp |
| RefSeq ORF | 834 bp |
| Locus ID | 836 |
| UniProt ID | P42574 |
| Cytogenetics | 4q35.1 |
| Domains | CASc, ICE_p10, ICE_p20 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protease |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis |
| MW | 31.6 kDa |
| Summary | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004346" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223662L3 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC223662L4 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, mGFP tagged | 10 ug |
$600.00
|
|
| RG223662 | CASP3 (tGFP-tagged) - Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha | 10 ug |
$489.00
$500.00
|
|
| SC117447 | CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha | 10 ug |
$300.00
|
|
Citations
Gasdermin E is required for induction of pyroptosis and severe disease during enterovirus 71 infection
Dong, Shi, Dong et al
J Biol Chem (2022) 298 (5), 101850 DOI: 10.1016/j.jbc.2022.101850
Dong, Shi, Dong et al
J Biol Chem (2022) 298 (5), 101850 DOI: 10.1016/j.jbc.2022.101850
Cited by 50
AI Summary: OriGene products contributed to this study by providing the full-length GSDME (RC210515) and caspase-3 (RC223662) plasmids, which were used to investigate the molecular mechanisms of pyroptosis during enterovirus 71 infection.
Snippet: midified atmosphere. EV71 infection was carried out as described previously (39, 40, 41). Plasmids and antibodies The GSDME (RC210515) and caspase-3 (RC223662) full-length plasmid was purchased from OriGene. The point mutation (D270A) was generated by PCR using KOD DNA polymerase (Toyobo). The plasmid that expresses EV71 3C or 2A was obtained as before description. The plasmids that express caspa
Targeting the XIAP/caspase-7 complex selectively kills caspase-3-deficient malignancies
Lin, Lai, Chang et al
J. Clin. Invest. (2013) 123 (9), 3861-75 DOI: 10.1172/JCI67951
Lin, Lai, Chang et al
J. Clin. Invest. (2013) 123 (9), 3861-75 DOI: 10.1172/JCI67951
Cited by 1
AI Summary: OriGene's pCMV-MIR vector was used to generate T47D cells stably expressing MIRLET7A1, enabling the study of microRNA-mediated caspase-3 downregulation and its impact on cancer cell apoptosis in this research.
Snippet: To generate T47D cells that stably expressed MIRLET7A1, T47D cells were transfected with the pCMV-MIR vector (Origene) containing a nontargeted ... The fluorescent protein band, visualized under a UV lamp, was excised from the gel and subjected to in-gel chymotryptic
Multiple ways to die: delineation of the unfolded protein response and apoptosis induced by Surfactant Protein C BRICHOS mutants
Maguire, Mulugeta, Beers
Int. J. Biochem. Cell Biol. (2012) 44 (1), 101-12 DOI: 10.1016/j.biocel.2011.10.003
Maguire, Mulugeta, Beers
Int. J. Biochem. Cell Biol. (2012) 44 (1), 101-12 DOI: 10.1016/j.biocel.2011.10.003
Cited by 59
Showing 1-3 of 3 papers.
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