GRB 14 (GRB14) (NM_004490) Human Untagged Clone

SKU SC117337

GRB14 (untagged)-Human growth factor receptor-bound protein 14 (GRB14)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GRB 14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004490, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACCACTTCCCTGCAAGATGGGCAGAGCGCCGCGAGCAGGGCGGCTGCCCGGGATTCGCCGCTGGCCG; CCCAGGTGTGTGGCGCTGCCCAGGGGAGGGGCGACGCCCACGACCTGGCGCCGGCCCCCTGGCTGCACGC; GCGAGCGCTCCTGCCCCTTCCGGACGGGACCCGCGGCTGTGCTGCAGACAGGAGAAAAAAGAAAGATCTT; GATGTTCCGGAAATGCCATCTATTCCAAACCCTTTTCCTGAGCTATGCTGTTCTCCATTTACATCTGTGT; TGTCAGCAGACCTATTTCCCAAAGCAAATTCAAGGAAAAAACAGGTGATTAAAGTATACAGTGAAGATGA; AACCAGCAGGGCTTTAGATGTACCCAGTGACATAACGGCTCGAGATGTTTGTCAGCTGTTGATCCTGAAG; AATCATTACATTGATGACCACAGCTGGACCCTTTTTGAGCACCTGCCTCACATAGGTGTAGAAAGAACAA; TAGAAGACCACGAACTGGTGATTGAAGTGCTATCCAACTGGGGGATAGAAGAAGAAAACAAACTATACTT; TAGAAAAAATTATGCCAAATATGAGTTCTTTAAAAACCCAATGTATTTTTTTCCAGAGCATATGGTATCT; TTTGCAACTGAAACCAATGGTGAAATATCCCCCACACAGATTTTGCAGATGTTTCTGAGTTCAAGCACAT; ATCCTGAAATTCATGGTTTCTTACATGCGAAAGAACAGGGAAAGAAGTCTTGGAAAAAAATTTACTTTTT; TCTAAGAAGATCTGGTTTATATTTTTCTACTAAAGGAACATCAAAGGAACCGCGGCATTTGCAGTTTTTC; AGCGAATTTGGCAATAGTGATATTTATGTGTCACTGGCAGGCAAAAAAAAACATGGAGCACCGACTAACT; ATGGATTCTGCTTTAAGCCTAACAAAGCGGGAGGGCCCCGAGACCTGAAAATGCTCTGTGCAGAAGAAGA; GCAGAGTAGGACGTGCTGGGTGACCGCGATTAGATTGCTTAAGTATGGCATGCAGCTGTACCAGAATTAT; ATGCATCCATATCAAGGTAGAAGTGGCTGCAGTTCACAGAGCATATCACCTATGAGAAGTATATCAGAGA; ATTCCCTGGTAGCAATGGACTTCTCAGGCCAGAAAAGCAGAGTTATAGAAAATCCCACTGAAGCCCTTTC; AGTTGCGGTTGAAGAAGGACTCGCTTGGAGGAAAAAAGGATGTTTACGCCTGGGCACTCACGGTAGCCCC; ACTGCCTCTTCACAGAGCTCTGCCACAAACATGGCTATCCACCGGTCCCAGCCATGGTTTCACCACAAAA; TTTCTAGAGATGAGGCTCAGCGATTGATTATTCAGCAAGGACTTGTGGATGGAGTTTTCTTGGTACGGGA; TAGTCAGAGTAACCCCAAAACTTTCGTACTGTCAATGAGTCATGGACAAAAAATAAAGCACTTTCAAATT; ATACCAGTAGAAGATGACGGTGAAATGTTCCACACACTGGATGATGGCCACACAAGATTTACAGATCTAA; TACAGCTGGTGGAGTTCTATCAACTCAATAAGGGCGTTCTTCCTTGCAAGTTGAAACATTATTGTGCTAG; GATTGCTCTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004490 unedited; NTTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGTCCTGG; CCTCCTGGCCCGGTGGGGACTGTCATGGCGGCCCAGCAACAGCTTAGGTGATCTCAGATG; GCAGAGCAGAAAGAATGCAAGGGTATGAGGGTCAGGGCTGCGCGGACCCCTATCCCGCCT; GCGGTCCTCCCGGCAAGCCCAGCGGGAGCGCCCGCTCGGCTGGGTCCCCGCCTCCAGCGG; CGCCGGGGCCGCCCAGACCCTGGGCTCAGCCTCGCGCCCCGGTGCCCACCTGAGGAGGCG; GCGGTCCCGGCCTCGCGTCCCGGATGGGACGGCGCGGGAGCAATGCCAGTGGCCCCGAGC; GCCCCGGGCCACGCGCGGGGCCGGCCAGCGCTTCTCGCGCCCCTTCCCGNCCCCTTCGCG; CCTTGCCTCGCCGNCCGCGGNCCCCACCCACCGGCCGCTCCTCCCCTCTCCCCACCCTTC; TNCTCCGNCCCCTTCCCTTCCCCNNCNNCTNNNNNNTNNCTCGGCCGCCCGTCCTCNCCC; CCTGTGCCCCGNTCGCTGGCGTCGTGGCCCCCCCCCCTGCCGTGCGCTCGGGCTTGCCTT; GCCGGCCCCTTGCCTTCCGTCGGTCCNTCCCCTTCTCCCCCCGCGCCGGTTTTCTCTTGT; TCCCTTGTCCCTTGGGTTGCCCCTTTGTCCCCTTTCCTGCNTTNTTGGCCTTCCCGCCTT; CCCCGGGCNGCTGCCCGGGCTTTCCCCTTGCCGCCCTGTTGNTGGGCCTTCCCCTGGGGG; GGGGTNCCCCCCCTCCCCCGCGGCGCGCCCTTGGTTGNCCNNCTTTCGTTTTTGGCCCTC; CGTCCGGGCCCCGGTTTGTCTTCTTCCCGGTGTTTTTGTCCCCTTTTTGTTCTGNTTTTC; CCTCTTTTCNCCCCTTTTNTTGTCCGCGTGGTGTNTCCCCTTTGTTTCGCTCCTTCTCCC; T;
• Restriction Sites: NotI-NotI
• ACCN: NM_004490
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004490.1, NP_004481.1
• RefSeq Size: 2376 bp
• RefSeq ORF: 1623 bp
• Locus ID: 2888
• UniProt ID: Q14449
• Cytogenetics: 2q24.3
• Domains: PH, RA, SH2
• Summary: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.