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NDUFC2 (NM_004549) Human Untagged Clone

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SKU
SC117270
NDUFC2 (untagged)-Human NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa (NDUFC2), nuclear gene encoding mitochondrial protein, transcript variant 1
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NDUFC2
Synonyms B14.5b; CI-B14.5b; HLC-1; NADHDH2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117270 sequence for NM_004549 edited (data generated by NextGen Sequencing)
ATGATCGCACGGCGGAACCCAGAACCCTTACGGTTTCTGCCGGATGAGGCCCGGAGCCTG
CCCCCGCCCAAGCTGACCGACCCGCGGCTCCTCTACATCGGCTTCTTGGGCTACTGCTCC
GGCCTGATTGATAACCTGATCCGGCGGAGGCCGATCGCGACGGCTGGTTTGCATCGCCAG
CTTCTATATATTACGGCCTTTTTTTTTGCTGGATATTATCTTGTAAAACGTGAAGACTAC
CTGTATGCTGTGAGGGACCGTGAAATGTTTGGATATATGAAATTACATCCAGAGGATTTT
CCTGAAGAAGATAAGAAAACATATGGTGAAATTTTTGAAAAATTCCATCCAATACGTTGA

Clone variation with respect to NM_004549.5
138 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004549 unedited
CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNATTCGGCACGAGGCAGAGGAGG
AGGAGAAAGCTGACCGCTTAGGCCCGGGTAGTGGTCGTCGTGGTTTTCCTTGTAGTTCGT
GGTCTGAGACCAGGCCTCAAGTGGAAACGGCGTCACCATGATCGCACGGCGGAACCCAGA
ACCCTTACGGTTTCTGCCGGATGAGGCCCGGAGCCTGCCCCCGCCCAAGCTGACCGACCC
GCGGCTCCTCTACATCGGCTTCTTGGGCTACTGCTCCGGCCTGATTGATAACCTGATCCG
GCGGAGGCCGATCGCGACGGCTGGTTTGCATCGCCAGCTTCTATATATTACGGCCTTTTT
TTTTGCTGGATATTATCTTGTAAAACGTGAAGACTACCTGTATGCTGTGAGGGACCGTGA
AATGTTTGGATATATGAAATTACATCCAGAGGATTTTCCTGAAGAAGATAAGAAAACATA
TGGTGAAATTTTTGAAAAATTCCATCCAATACGTTGAAGTCTTCAAAATGCTTGCTCCAG
TTTCACTGATACCTGCTGTTTCTGAATTTGATGGAACATGTTTCTTATGACAGTTGAAGC
TTATGCTAATCTGTATGTTGACACCTTGTAATTAAAATACGTACCATGAGTACTCTGACT
CTTTATTGTTGTAAGAAAAATAGTTCATCATCAGAGAATGGTCAGTTTCCATTCACCCAT
CTGAAAGATTNTAAGATGAGGTTGAATTCTAGTTTAGAGTTAGCAGACTACCCAAATCTG
GCCCACTGCCAANTAAGTCTCATTGGGACACAGCCATGCTCATTTGTTTACATGTNGNTT
ATGGCGACTGCCATGCTGCAGAACAGAATGAGTAGNTGTGATAGAGACTATGGGCCTGCA
ACCTAATNATATGTAGCCCTTCAAAAAGGTNTGCCTGCCCTGTCTATGCAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004549 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTGTATTTGTACATCTTTATTATTTCTAAAGCAC
TTTCCTCAACCTAATTTCAGTTTTTACAATTGGTACTCAAGAAAATAGAGACAGAAATCA
TTTGATTTTGCCCAGAAACCATCTGCTTATATTTATAAGGCCACCTAATTTGAAATCACA
TATAGACCAGGCGCGGTGGCTCACGCCTGTAATTCCAACACTTTGGAAGGCCAAGGCAGG
TGGATCACAAGGTCAAGAGATTGAGACCATCTTGGCCAACATGGTGAAACCCCGTCTGCA
CTAAAAACCACAAAAATCACCTGGGCGTCCTGGTATGCCCCCCGTATTTTTACCCACTTC
GGCAGGCTGTCGCTCGCATACCCTCTGTCCCCTCTAGCCCCGGTCCTCCCGTTCCCCCCC
GCCCCCCTGAACTAACCCCCGTTTACCCTTTTCTACGCCTTTCCTCTTTCCTTTTCCCTC
CCTTCCCTTTCCCCCTTTCCCCGTTCTTCCTTTCTTTCCCTCCCGATTCTATCTCCCCCA
TTCCCATCCCCATTCCTTTTTTTCCCCTCCCATCCCCTTTCCCCCCCTTTACTTATACCA
CATCCCCCCCACTCACCGTTCCCTCCTTTTATCCACCTTCTTTCCCCTGTCCTCTCTCAC
TCTATCCCATCCCCCCCATCCCTTTCCCCCTTCCCCTCCATCCATTCTTTCCCTCCCCCT
CTCGATCTCTTTTTTGTTCCCTCTTCCCCTCCTTTCTCTTTTTCCTTCCCCCCCCTTTTT
TTCCCTTTTCCCCTCCTTCCCCCCGCTCTCCTTCCCTCCCCTTTTCCCCGCCTTCTCGTT
TTCTTGTTCCCCTCCCCCCTCCCCCAATGCCTTTGTCCCCATCTTCCTCCCATCCCTCAT
ATCCCTTCGCCATTTTTCTCCCATTATCCGCGCCTCCCTCTCCCTCTCTTCTCTCCCTCA
TGTCCCCAATCCTTAATCCGTCAACCGCTCACGCCACTATTCCTCTTCCCATCTCCTTTC
CC
Restriction Sites NotI-NotI |
ACCN NM_004549
Insert Size 2190 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004549.3, NP_004540.1
RefSeq Size 2159 bp
RefSeq ORF 360 bp
Locus ID 4718
UniProt ID O95298
Cytogenetics 11q14.1
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_004549" in other vectors (4)
SKU Description Size Price
RC200325 NDUFC2 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa (NDUFC2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$150.00
RC200325L3 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa (NDUFC2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC200325L4 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa (NDUFC2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$450.00
RG200325 NDUFC2 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa (NDUFC2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00

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