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AP3B2 (NM_004644) Human Untagged Clone

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SKU
SC117246
AP3B2 (untagged)-Human adaptor-related protein complex 3, beta 2 subunit (AP3B2)
  $943.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol AP3B2
Synonyms DEE48; EIEE48; NAPTB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117246 sequence for NM_004644 edited (data generated by NextGen Sequencing)
ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCC
GAGTACGGCCACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGAT
GACCTGAAGGAGATGCTGGACACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGG
ATTGTGGCGATGATTGCCCGAGGAAAGAATGCTTCAGACCTGTTTCCCGCGGTGGTGAAG
AACGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCTATGTGTACCTGGTACGCTAC
GCTGAGGAGCAGCAAGACCTGGCCCTGCTGTCCATCTCCACCTTCCAACGTGGCCTAAAG
GATCCCAACCAGCTGATTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCC
ATCATAGTGCCCATCATGATGCTAGCTATCAAGGAAGCCGCCTCGGACATGTCACCCTAT
GTGCGGAAAACAGCTGCCCACGCCATCCCTAAACTCTACAGTTTGGACTCTGACCAGAAG
GATCAGCTGATAGAAGTCATTGAGAAGCTTCTGGCTGACAAGACCACGCTGGTGGCGGGC
AGTGTGGTGATGGCCTTTGAGGAGGTCTGCCCGGAGCGCATCGACCTGATTCACAAAAAC
TACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGTGGGGCCAGGTGGTCATCATC
AGCATGCTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAACGAATCCCTA
CTAGAGGAGAACGCGGAAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCG
GGGTCTGAGGAGACGGCCGCCGCGGCCGCCCCCTCCCGAAAGCCCTATGTCATGGACCCC
GACCACCGGCTGCTGCTGCGCAACACCAAACCCCTGCTGCAGAGCCGCAGCGCCGCGGTG
GTGATGGCGGTGGCGCAGCTCTACTTCCACCTGGCGCCCAAGGCGGAAGTGGGCGTCATC
GCCAAGGCGCTGGTGCGCCTGCTGCGCAGCCACAGTGAGGTGCAGTACGTTGTGCTCCAG
AACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTGAGCCCTACCTGAAGAGCTTC
TACATCAGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGTGCTGACCAAC
CTGGCCAATGAGACCAACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGC
ATGGACAAGGACTTTGTGGCAGCCACAATCCAGGCCATTGGACGCTGTGCAACTAACATC
GGCCGAGTCCGTGACACCTGCCTCAATGGCCTGGTGCAGCTGCTGTCCAACCGTGATGAG
CTTGTGGTTGCAGAGTCAGTGGTCGTCATTAAGAAATTGCTACAGATGCAGCCAGCACAA
CATGGAGAGATCATCAAACACTTGGCAAAGCTTACAGACAACATCCAGGTGCCCATGGCC
CGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGCATGTCCCCAGGATTGCACCT
GATGTCTTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGTCAAGCTGCAG
GTCATCAACCTGGCAGCCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACC
CAGTATGTGCTGAGTCTGGCCAAATATGACCAGAACTATGATATTCGCGACCGGGCGCGC
TTCACCCGGCAGCTCATCGTCCCTTCCGAGCAGGGTGGGGCCCTCAGCCGCCATGCCAAG
AAGCTCTTCCTGGCACCCAAACCAGCTCCAGTCTTGGAGTCATCCTTCAAAGACCGGGAC
CACTTCCAGCTGGGCTCACTGTCCCACCTGCTTAATGCCAAGGCCACAGGCTACCAGGAG
CTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGCGCAACGTGGAGGTACCTGAA
TGGACCAAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTTCTACTCGGAC
TCTGAGGGGGAGTCAGGCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAA
TCGGACAGTAAGAGCAGCAGTGAGAGCGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAAT
GAAGACCAGGATGAGGATGAGGAGAAAGGGAGAGGCAGTGAGAGTGAACAGAGTGAGGAG
GATGGTAAGAGGAAGACAAAGAAGAAGGTGCCAGAGAGAAAAGGAGAAGCGTCATCCTCT
GATGAGGGCAGCGATTCCAGCAGTAGCTCATCAGAGTCCGAGATGACATCGGAGTCCGAG
GAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACACCTCCCAGCAGCAAAAGTGCT
CCTGCAACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCCCAGTGTCCAG
CCTGTGTCTCCCCCAGCAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACA
CTCACAGACTCCACCCTGGTACCGTCGCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAG
GAGCTGCTGCACCGGGTAGCTGGCGAGGGGCTGGCTGTGGACTACACCTTCAGCCGCCAA
CCTTTCTCCGGGGATCCCCACATGGTGTCCGTGCACATCCACTTCTCCAACAGCTCTGAT
ACCCCCATCAAGGGCCTGCATGTGGGCACTCCCAAACTGCCTGCTGGCATCAGCATCCAA
GAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGCCACTGCTGTAATGGGCATT
AATTTCTGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGACCCGACAGTTC
TACGTCTCCATTCAGCCACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAAT
GAGTTTAAGAAGGAACAGGGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATG
CTGCCAGACACCTGTCGGAGTGACCACATTGTGGTGCAGAAAGTGACTGCCACTGCCAAC
CTGGGTCGTGTTCCTTGTGGGACATCTGATGAGTACAGGTTTGCAGGGAGGACACTGACT
GGTGGAAGCCTCGTTCTGCTGACCCTGGATGCCCGGCCAGCTGGAGCTGCCCAGCTGACT
GTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAAAGGATGTGATACAGGCTCTG
ACCCAGTGA

Clone variation with respect to NM_004644.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004644 unedited
TTTTCGGAATTTGAAACACGATCTCACTATAGGGCGGCACGCGCAATCGGCACGAGGCCC
TGCCGCGCCGCCGCCTTCGCCAGCCCGGACCGGACCGGCCCGCCTTCCCTTCCTCCGCTC
CAGCCGGCCTCCGGCCCCGCAACCTCCTCCTCGGCGAAGTCTCCCTGGCCGCCCCATGTC
GGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGTTCCGCTGGCCCCGGGGAGCCCGAGTA
CGGCCACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCT
GAAGGAGATGCTGGACACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGT
GGCGATGATTGCCCGAGGAAAGAATGCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGT
GGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCTATGTGTACCTGGTACGCTACGCTGA
GGAGCAGCAAGACCTGGCCCTGCTGTCCATCTCCACCTTCCAACGTGGCCTAAAGGATCC
CAACCAGCTGATTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCCATCAT
AGTGCCCATCATGATGCTAGCTATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCG
GAAAACAGCTGCCCACGCCATCCCTAAACTCTACAGTTTGGACTCTGACCAGAGGATCAG
CTGATAGAAGTCATTGAGAAGCTTCTGGCTGACAAGACCACGCCTGTGGCGGCCAGTGTG
GTGATGGCCTTTGAGGAGTCTGCCCGGAGCGCATCGACCTGATTCACCAAACTACCGGAA
CTCTGTACCCTGCTGATCGACTGGNAGAATGGGGCCAAGTGTTCATCATCAGCATGCTCA
CCCGNTACGCCGGACGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004644 unedited
GACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGAGAAGACATTTT
ATTGAGCCTGCTACATAAATAGCTACAGAAATCTGGGAGGACTGAAGGGAGTGGCTGCCA
TCTCTCTCTGCACAGATCACTAAGGAATCCATGGGGAGGGCATTAGGGGAGGGCTTGGTC
CTCCAGAGGGAGAGAGGACACCCTGAATGCTATCTGGCATGAGGAGGATGATGAGAGAGA
GAGAGAAAGATGAGAGAGACTGACAGCCTAGGTGTCATGGGGAGGTATAGATGGGAGCCA
AACAGGTCACAGCATTTGGAAGTCACTGGGTCAGAGCCTGTATCACATCCTTTACCAGCA
TGGTGCCAATCACCATTTTCTCGCTGTTGACAGTCAGCTGGGCAGCTCCAGCTGGCCGGG
CATCCAGGGTCAGCAGAACGAGGCTTCCACCAGTCAGTGTCCTCCCTGCAAACCTGTACT
CATCAGATGTCCCACAAGGAACACGACCCAGGTTGGCAGTGGCAGTCACTTTCTGCACCA
CAATGTGGTCACTCCGACAGGTGTCTGGCAGCATGAGTTTCTCTGTGATCTCATTCATGC
CCATCAGCTTTCCCTGTTCCTTCTTAAACTCATTTTCACTCATGAACACAGGGGCCATCA
GCTCCCCAACANGTGGCTGAATGGAGACGTAAAACTGTCGGGTCTTGGGTGCACAGCTGG
AAAGTTGGCTGCCTGGGTTTGAGTCACAGAAAATAATGCCCCTTACAGCAGTGGCAGATT
CTTCCAGTGCCAGGGACTCANATTCGGGAAATCTTTGATGCTGATGCANCAAGCAGTTGG
GAGTGCCCACTGCCAGCCCTTGAGGGGGATCAAANCTGTGGAGAAATGGATGCCCCGCAC
CTTTGGGGATCCCGGAAAGGTGGCGCTGAA
Restriction Sites NotI-NotI |
ACCN NM_004644
Insert Size 3760 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004644.3, NP_004635.2
RefSeq Size 3746 bp
RefSeq ORF 3249 bp
Locus ID 8120
UniProt ID Q13367
Cytogenetics 15q25.2
Domains Adaptin_N
Protein Pathways Lysosome
Summary Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. provided by RefSeq, Feb 2017
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
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"NM_004644" in other vectors (6)
SKU Description Size Price
RC210820 AP3B2 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, beta 2 subunit (AP3B2) 10 ug
$942.00
RC210820L1 Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), Myc-DDK-tagged 10 ug
$1,242.00
RC210820L2 Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), mGFP tagged 10 ug
$1,242.00
RC210820L3 Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), Myc-DDK-tagged 10 ug
$1,242.00
RC210820L4 Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), mGFP tagged 10 ug
$1,242.00
RG210820 AP3B2 (tGFP-tagged) - Human adaptor-related protein complex 3, beta 2 subunit (AP3B2) 10 ug
$789.00 $1,142.00

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