MTA1 (NM_004689) Human Untagged Clone

SKU SC117199

MTA1 (untagged)-Human metastasis associated 1 (MTA1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: MTA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117199 sequence for NM_004689 edited (data generated by NextGen Sequencing); ATGGCCGCCAACATGTACAGGGTCGGAGACTACGTCTACTTTGAGAACTCCTCCAGCAAC; CCATACCTGATCCGGAGAATCGAGGAGCTCAACAAGACGGCCAATGGGAACGTGGAGGCC; AAAGTGGTGTGCTTCTACCGGAGGCGGGACATCTCCAGCACCCTCATCGCCCTGGCCGAC; AAGCACGCAACCCTGTCAGTCTGCTATAAGGCCGGACCGGGGGCGGACAACGGCGAGGAA; GGGGAAATAGAAGAGGAAATGGAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAG; CACCAGCTGCGGCATCGGGAGCTGTTCCTCTCCCGGCAGCTGGAGTCTCTGCCCGCCACG; CACATCAGGGGCAAGTGCAGCGTCACCCTGCTCAACGAGACCGAGTCGCTCAAGTCCTAC; CTGGAGCGGGAGGATTTCTTCTTCTATTCTCTAGTCTACGACCCACAGCAGAAGACCCTG; CTGGCAGATAAAGGAGAGATTCGAGTAGGAAACCGGTACCAGGCAGACATCACCGACTTG; TTAAAAGAAGGCGAGGAGGATGGCCGAGACCAGTCCAGGTTGGAGACCCAGGTGTGGGAG; GCGCACAACCCACTCACAGACAAGCAGATCGACCAGTTCCTGGTGGTGGCCCGCTCTGTG; GGCACCTTCGCACGGGCCCTGGACTGCAGCAGCTCCGTCCGACAGCCCAGCCTGCACATG; AGCGCCGCAGCTGCCTCCCGAGACATCACCCTGTTCCACGCCATGGATACTCTCCACAAG; AACATCTACGACATCTCCAAGGCCATCTCGGCGCTGGTGCCGCAGGGCGGGCCCGTGCTC; TGCAGGGACGAGATGGAGGAGTGGTCTGCATCAGAGGCCAACCTTTTCGAGGAAGCCCTG; GAAAAATATGGGAAGGATTTCACGGACATTCAGCAAGATTTTCTCCCGTGGAAGTCGCTG; ACCAGCATCATTGAGTACTACTACATGTGGAAGACCACCGACAGATACGTGCAGCAGAAA; CGCTTGAAAGCAGCTGAAGCTGAGAGCAAGTTAAAGCAAGTTTATATTCCCAACTATAAC; AAGCCAAATCCGAACCAAATCAGCGTCAACAACATCAAGGCCGGTGTGGTGAACGGCACG; GGGGCGCCGGGCCAGAGCCCTGGGGCTGGCCGGGCCTGCGAGAGCTGTTACACCACACAG; TCTTACCAGTGGTATTCTTGGGGTCCCCCTAACATGCAGTGTCGTCTCTGCGCATCTTGT; TGGACATATTGGAAGAAATATGGTGGCTTGAAAATGCCAACCCGGTTAGATGGAGAGAGG; CCAGGACCAAACCGCAGTAACATGAGTCCCCACGGCCTCCCAGCCCGGAGCAGCGGGAGC; CCCAAGTTTGCCATGAAGACCAGGCAGGCTTTCTATCTGCACACGACGAAGCTGACGCGG; ATCGCCCGGCGCCTGTGCCGTGAGATCCTGCGCCCGTGGCACGCTGCGCGGCACCCCTAC; CTGCCCATCAACAGCGCGGCCATCAAGGCCGAGTGCACGGCGCGGCTGCCCGAAGCCTCC; CAGAGCCCGCTGGTGCTGAAGCAGGCGGTACGCAAGCCGCTGGAAGCCGTGCTTCGGTAT; CTTGAGACCCACCCCCGCCCCCCCAAGCCTGACCCCGTGAAAAGCGTGTCCAGCGTGCTC; AGCAGCCTGACGCCCGCCAAGGTGGCCCCCGTCATCAACAACGGCTCCCCCACCATCCTG; GGCAAGCGCAGCTACGAGCAGCACAACGGGGTGGACGGCAACATGAAGAAGCGCCTCTTG; ATGCCCAGTAGGGGTCTGGCAAACCACGGACAGACCAGGCACATGGGACCAAGCCGGAAC; CTCCTGCTCAACGGGAAGTCCTACCCCACCAAAGTGCGCCTGATCCGGGGGGGCTCCCTG; CCCCCAGTCAAGCGGCGGCGGATGAACTGGATCGACGCCCCGGATGACGTGTTCTACATG; GCCACAGAGGAGACCAGGAAGATCCGCAAGCTGCTCTCATCCTCGGAAACCAAGCGTGCT; GCCCGCCGGCCCTACAAGCCCATCGCCCTGCGCCAGAGCCAGGCCCTGCCGCCGCGGCCA; CCGCCACCTGCGCCCGTCAACGACGAGCCCATCGTCATCGAGGACTAG; ; Clone variation with respect to NM_004689.3; 1114 g=>a;1834 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004689 unedited; AGAGTCGCAATTTGTATACCACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGGCCC; TCCCGTCCCTGCGCGGCCTCGGCGGCCTCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCA; GCGCGGCCCCTTTAAACGCCTGCGGCGCCCCCCGCCCCCGCCATCGCGCCTCCATTTTCC; CGGCCGCCCGCGCCGAGCGCCGCGCCCGCCCCGGGCCCCTCCGCCGCCGCCGGCCCGGAC; ATGGCCGCCAACATGTACAGGGTCGGAGACTACGTCTACTTTGAGAACTCCTCCAGCAAC; CCATACCTGATCCGGAGAATCGAGGAGCTCAACAAGACGGCCAATGGGAACGTGGAGGCC; AAAGTGGTGTGCTTCTACCGGAGGCGGGACATCTCCAGCACCCTCATCGCCCTGGCCGAC; AAGCACGCAACCCTGTCAGTCTGCTATAAGGCCGGACCGGGGGCGGACAACGGCGAGGAA; GGGGAAATAGAAGAGGAAATGGAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAG; CACCAGCTGCGGCATCGGGAGCTGTTCCTCTCCCGGCAGCTGGAGTCTCTGCCCGCCACG; CACATCAGGGGCAAGTGCAGCGTCACCCTGCTCAACGAGACCGAGTCGCTCAAGTCCTAC; CTGGAGCGGGAGGATTTCTTCTTCTATTCTCTAGTCTACGACCCACAGCAGAAGACCCTG; CTGGCAGATAAAGGAGAGATTCGAGTAGGAAACCGGTACCAGGCAGACATCACCGACTTG; TTAAAAGAAGGCGAGGAGGATGGCCGAGACCAGTCCAGGTTGGAGACCCAGGTGTGGGAG; GCGCACAACCCACTCACANGACAGCAGATCGACCAGTTCCTGGGTGGTGGCCCGCTCTGT; GGGCACCTTCGCACGGGCCCTGGACTGCAGCAGCTCCGTCCGACAGCCCAGCCTGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004689 unedited; ACCGCGGCCGCATCTACATCGGTTTTTTTTTTTTTTTTTTGTTAATATGAAAATTACTTG; AAAAGACAAGGGCCAACCCCGCCAGGCAGCTCGGCCGGCCGCGGCCGCACGATCCTAACA; TTCCAGGTGTAAGTTACAGAGCTCAAGTTCATCTACAAAAAAAGTAATAAAAATAAAATT; TAAAATGGCACCTTCAACAGAACACAACAAAACCTTAGGGCCCGGCCTCCGGGCAAAAGG; CCCCGCAGCACAGGGGCACGGGGTGGCCCCTCATCTCCGCTCCAGCCAAAAAGTAAACAC; AAAGCTAAAAACGACAACTCCTCGGCATTCTCGGAATAAGTTAGCCGCGCTTCTTCCTCT; CCTCCCCCAGTGTCCGCCAAGGAGCGCGTTTCTCTGCAGGTGAGGGCGGCACGTGGGCGC; ACTACCAAACTGAGGGGCGGGCGGCGGGCTGGCTGGGAAGGGGCCCCGCGGGCGGGCCAG; GGGCGGGGGGCGGACGCAAGTGGGGGCGGACCCTAGTCCTCCATGACGATGCGCTCGTCC; CTGACGGCCGCATGTGGCGGCGGCCGCGGCGGCACGGTCTTGCTCTGGCCACCGCCATGG; GCTTGTCCGGCCGCCGGCAACTACGCTTTGCTCCCGAGACGACAGCACCTTGCGGATCTT; TCTTGGCCTCCCCCTGGGCCCTGACAACCAGCCACCCGGCCCGTTGATCATTTCACCGCC; TCCGTTTGACTGGCGCATGGACCCTCCTCGATCAAGCCAATTCGGCGGGTACGACTTCCC; GCTTACACGCAGTCACGGCTTGCCCCATGGCCCGCCCGCCCGCGTTTTCCCCCCCCCTAT; GGGTTTTACCAGCCCCTCTTTATGGTTCCCCCTCACCCGCCTTGCTCGCCCCACTCCCCT; TGCCAAAAGCGTGGCAAACCGTCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004689
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004689.2, NP_004680.1
• RefSeq Size: 2662 bp
• RefSeq ORF: 2148 bp
• Locus ID: 9112
• UniProt ID: Q13330
• Cytogenetics: 14q32.33
• Domains: BAH, ELM2, GATA, myb_DNA-binding
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (MTA1).