LARGE (LARGE1) (NM_004737) Human Untagged Clone
$693.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | LARGE |
| Synonyms | LARGE; MDC1D; MDDGA6; MDDGB6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004737 |
| Insert Size | 4110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004737.3, NP_004728.1 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 9215 |
| UniProt ID | O95461 |
| Cytogenetics | 22q12.3 |
| Domains | Glyco_transf_8 |
| Protein Families | Druggable Genome, Transmembrane |
| Summary | This gene encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. The protein encoded by this gene is the glycotransferase that adds the final xylose and glucuronic acid to alpha-dystroglycan and thereby allows alpha-dystroglycan to bind ligands including laminin 211 and neurexin. Mutations in this gene cause several forms of congenital muscular dystrophy characterized by cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in multiple transcript variants that encode the same protein. provided by RefSeq, May 2018 Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004737" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217876 | LARGE (Myc-DDK-tagged)-Human like-glycosyltransferase (LARGE), transcript variant 1 | 10 ug |
$692.00
|
|
| RC217876L3 | Lenti ORF clone of Human like-glycosyltransferase (LARGE), transcript variant 1, Myc-DDK-tagged | 10 ug |
$992.00
|
|
| RC217876L4 | Lenti ORF clone of Human like-glycosyltransferase (LARGE), transcript variant 1, mGFP tagged | 10 ug |
$992.00
|
|
| RG217876 | LARGE (tGFP-tagged) - Human like-glycosyltransferase (LARGE), transcript variant 1 | 10 ug |
$892.00
|
|
Citations
Dystroglycan Binding to α-Neurexin Competes with Neurexophilin-1 and Neuroligin in the Brain
Reissner, Stahn, Breuer et al
J. Biol. Chem. (2014)
Reissner, Stahn, Breuer et al
J. Biol. Chem. (2014)
AI Summary: In this study, the full-length human LARGE plasmid (pCMV6-XL4 LARGE) from OriGene Technologies was used to investigate how LARGE-dependent glycans influence the binding interactions between α-neurexin and its synaptic partners.
Snippet: 36);rat Nlgn2, pCMVNL2-1 (27);full- Page 3. α-Neurexin multiplexes 3 length rat DAG, pCMVDAG (60);full-length human LARGE, pCMV6-XL4 LARGE (OriGene Technologies). Novel plasmids generated for this study include ... Cite SaveSaving
Overexpression of LARGE suppresses muscle regeneration via down-regulation of insulin-like growth factor 1 and aggravates muscular dystrophy in mice
Saito, Kanagawa, Ikeda et al
Hum. Mol. Genet. (2014)
Saito, Kanagawa, Ikeda et al
Hum. Mol. Genet. (2014)
Cited by 24
AI Summary: OriGene’s human LARGE cDNA was used in this study to overexpress the LARGE gene in cell experiments, enabling the researchers to investigate how increased LARGE levels affect muscle regeneration and IGF-1 expression in muscular dystrophy models.
Snippet: 37 °C incubator with 5% CO2 and 95% air. Human LARGE cDNA was obtained from OriGene and cloned into pCMV-FLAG-MAT-Tag-2 (Sigma-Aldrich) for transient transfection of cells. C2C12 and HEK293 cells were transfected using Effectene (Qiagen). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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