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DREF (ZBED1) (NM_004729) Human Untagged Clone

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SKU
SC117180
ZBED1 (untagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2
  $636.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DREF
Synonyms ALTE; DREF; hDREF; TRAMP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117180 sequence for NM_004729 edited (data generated by NextGen Sequencing)
ATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCACCCCCGC
GCCAAGAGCAAGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATGCATCCTG
CAGTGGAAGAAAATCTACTGCCGCATCTGCATGGCCCAGATCGCCTACTCCGGAAACACC
TCCAACCTGTCCTACCACCTGGAGAAGAACCACCCCGAGGAATTCTGCGAGTTCGTCAAG
AGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCTTCTCCAAGCTGAAGCCCGAG
TCGTCCCAGCAGCCCGGGCAGGACGCGCTGGCCGTCAAGGCCGGCCACGGCTACGACAGC
AAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCTGTACCCA
GCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCCCCGGTAT
GAGCTGCCCAGCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGGGGCCGTC
CGGGAGGTGATCCTGAAGGAGCTGGCCGAGGCCACCTGGTGTGGCATCTCCACCGACATG
TGGAGGAGTGAGAATCAGAACCGCGCCTACGTCACGCTGGCCGCCCACTTCCTGGGCCTG
GGCGCCCCCAACTGCCTGTCCATGGGCTCCCGCTGCCTGAAGACCTTCGAGGTGCCCGAA
GAGAACACGGCGGAGACCATCACGCGAGTGCTCTATGAGGTCTTCATCGAGTGGGGCATC
AGCGCCAAGGTCTTCGGGGCCACCACCAACTATGGCAAGGACATCGTGAAGGCGTGCTCC
CTGCTGGACGTCGCAGTGCACATGCCCTGCCTGGGCCACACCTTCAATGCCGGCATCCAG
CAGGCCTTCCAGCTCCCGAAGCTGGGGGCGCTGCTGTCGCGCTGCCGCAAACTGGTGGAG
TACTTCCAGCAGTCTGCCGTGGCCATGTACATGCTCTATGAGAAGCAGAAGCAGCAGAAC
GTGGCCCACTGCATGCTGGTGAGCAACCGCGTCTCCTGGTGGGGGAGCACGCTGGCCATG
CTGCAGCGCCTCAAGGAGCAGCAGTTCGTCATCGCCGGGGTCTTGGTGGAGGACAGCAAC
AACCACCACCTCATGCTGGAGGCCAGCGAGTGGGCCACCATCGAGGGGCTGGTGGAGCTC
CTGCAGCCCTTCAAGCAGGTGGCCGAGATGCTGTCGGCCTCCAGGTACCCCACCATCAGC
ATGGTGAAGCCGCTGCTGCACATGCTCCTGAACACCACGCTCAACATCAAGGAGACCGAC
TCCAAGGAGCTCAGCATGGCCAAGGAGGTCATCGCCAAGGAGCTTTCCAAGACCTACCAG
GAGACGCCCGAGATCGACATGTTTCTCAACGTGGCCACCTTCCTGGACCCCCGCTACAAG
AGGCTGCCCTTCCTCTCCGCCTTCGAGCGGCAGCAGGTGGAGAATCGCGTGGTGGAAGAG
GCCAAGGGCCTGCTGGACAAGGTCAAAGACGGCGGCTACCGGCCGGCTGAGGACAAGATC
TTCCCGGTGCCCGAGGAGCCTCCCGTCAAGAAGCTCATGCGGACATCCACGCCGCCGCCC
GCCAGCGTCATCAACAACATGCTGGCCGAGATCTTCTGCCAGACAGGCGGCGTGGAGGAC
CAGGAAGAGTGGCATGCCCAGGTGGTGGAGGAGCTGAGCAACTTCAAGTCCCAGAAGGTG
CTTGGCCTCAACGAAGACCCCCTCAAGTGGTGGTCAGACCGCCTGGCCCTCTTCCCCCTG
CTGCCCAAGGTGCTGCAGAAGTACTGGTGCGTGACGGCCACGCGCGTCGCCCCTGAGCGT
CTCTTCGGATCCGCCGCCAACGTGGTCAGCGCCAAGAGGAACCGGCTGGCTCCCGCGCAC
GTGGACGAGCAGGTGTTTCTGTATGAGAACGCCCGGAGTGGGGCAGAGGCGGAACCCGAG
GACCAGGACGAGGGGGAGTGGGGCCTGGACCAGGAGCAGGTGTTCTCCTTGGGGGATGGC
GTCAGCGGCGGTTTCTTTGGCATTAGGGACAGCAGCTTCCTGTAG

Clone variation with respect to NM_004729.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004729 unedited
GGATTTTGTATCACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGCGGGGG
CTTCCTGGAGCCAGCAGGTCCCCACGCATGAGCAGCCGTCCAGCAGGCAGGCTCCGGTGG
AGAAGCAATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCA
CCCCCGCGCCAAGAGCAAGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATG
CATCCTGCAGTGGAAGAAAATCTACTGCCGCATCTGCATGGCCCAGATCGCCTACTCCGG
AAACACCTCCAACCTGTCCTACCACCTGGAGAAGAACCACCCCGAGGAATTCTGCGAGTT
CGTCAAGAGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCTTCTCCAAGCTGAA
GCCCGAGTCGTCCCAGCAGCCCGGGCAGGACGCGCTGGCCGTCAAGGCCGGCCACGGCTA
CGACAGCAAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCT
GTACCCAGCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCC
CCGGTATGAGCTGCCCAGCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGG
NGCCGTCCGGGAGGTGATCCTGAANGAGCTGGCCGANGCCACCCTGGTGTGGCATCTCCA
CCGACATGTGGAGGAGTGAGAATCAGAACCGCGCCTACGTCACGCTGGCCGCCCACTTCC
TGGGCCTGNGCGCCCCCCACTGCCTGTCCATGGGCTTCCGCTGCCTGAAGACCTTCGAGT
GCCCGAAGAGAAACACGCGAGACCTCACGCGAGTGCTCTATGAGTCCTCATCGAGTGGGC
ATCAGCGCAAGTCTCCGNGCCACACCACTATGCAGGACATGGAAGCTGCTCCTGCTGAG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004729 unedited
GAACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAGATCTCGCTC
TTGTCGCCCAGGCTGGAGTGCAGCGATCTCAGCTTACTGCAACCTCCACCTCCCAGGTTC
AAGCGATTCTCTGGCCTCAGCCTCCAGAAGCTGGGATTACAGGCACATGCCATCAGTCCT
GGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCTCG
ATCTCCTGACCTCAGCTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGCGATTATAGACA
GGAGCCACCGCCCCCGACCCTCTCTCACTTCTCGAATCTCTTTCCTTTTTCCACCTTCTA
GGTGTCAAAGACAGTGGATGGTCTCTGAGGTTCAAAACCAAGCTGACCGGGTAAGTATTT
ACAGCAAAGCATCCAATGGGCTGCTGCGGGGATGACTTGTAAGACAACACGCTTCCTCGC
TACAGGAAGCTGCTGTCCCTAATGCCAAAGAAACCGCCGCTGACGCCATCCCCCAAGGAG
AACACCTGCTCCTGGTCCAGGCCCCACTCCCCCTCGTCCTGGTCCTCGGGTTCCGCCTCT
GCCCCACTCCGGGCGTTTTCATACAGAAACACCTGCTCGTTCACGTGCGCCGGAGCCCAC
CCGTTCCCTCTTGGCCCTGACCACCCTCGCCGCGGATCCCCAACAAACCCTCACGGGCCA
CCCCCCCTGGCCGCCCACCCACCAAATTCCTTCGCCGTCCCTTGGGCTACAAGGGGATAA
AAGGCCCCTCCGGGCCCAACCCCACTCTAGAGGGTACTTCCGTGTGCCCCCTACCCTCCT
TCGGCACTAGAACCTCCTATCGTCTCCCCCCCCTGTACTTCTCCTCTTTCCAGCTCCTAA
CCCTCGCCTCCCACCCCAAACATCCGAGCACGTACGCGACATCAATCAGCGC
Restriction Sites NotI-NotI |
ACCN NM_004729
Insert Size 2680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004729.3, NP_004720.1
RefSeq Size 4525 bp
RefSeq ORF 2085 bp
Locus ID 9189
UniProt ID O96006
Cytogenetics X;Y
Domains zf-BED
Protein Families Druggable Genome
Summary This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. provided by RefSeq, Jan 2010
Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon compared to variant 1. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_004729" in other vectors (4)
SKU Description Size Price
RC202011 ZBED1 (Myc-DDK-tagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2 10 ug
$636.00
RC202011L3 Lenti ORF clone of Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2, Myc-DDK-tagged 10 ug
$936.00
RC202011L4 Lenti ORF clone of Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2, mGFP tagged 10 ug
$936.00
RG202011 ZBED1 (tGFP-tagged) - Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2 10 ug
$836.00

file-alt Citations

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