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B4GALT5 (NM_004776) Human Untagged Clone

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SKU
SC117170
B4GALT5 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol B4GALT5
Synonyms B4Gal-T5; BETA4-GALT-IV; beta4Gal-T5; beta4GalT-V; gt-V
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117170 sequence for NM_004776 edited (data generated by NextGen Sequencing)
ATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCGCCGCTCGCTGCTCGCCGCGCTCTTC
TTCTTTTCTCTCTCGTCCTCGCTGCTGTACTTCGTCTATGTGGCGCCCGGCATAGTGAAC
ACCTACCTCTTCATGATGCAAGCCCAAGGCATTCTGATCCGGGACAACGTGAGAACAATC
GGTGCTCAGGTTTATGAGCAGGTGCTTCGGAGTGCTTATGCCAAGAGGAACAGCAGTGTA
AATGACTCAGATTATCCTCTTGACTTGAACCACAGTGAAACCTTCCTGCAAACTACAACA
TTTCTTCCTGAAGACTTCACCTACTTTGCAAACCATACCTGCCCTGAAAGACTCCCTTCC
ATGAAGGGCCCAATAGACATAAACATGAGTGAAATTGGAATGGATTACATTCATGAACTC
TTCTCCAAAGACCCAACCATCAAGCTCGGAGGTCACTGGAAGCCTTCTGATTGCATGCCT
CGGTGGAAGGTGGCGATCCTTATCCCCTTCCGGAACCGCCACGAGCACCTCCCAGTCCTG
TTCAGACACCTGCTTCCCATGCTCCAGCGCCAGCGCCTGCAGTTTGCATTTTATGTGGTT
GAACAAGTTGGTACCCAACCCTTTAATCGAGCCATGCTTTTCAACGTTGGCTTTCAAGAA
GCAATGAAAGACTTGGATTGGGACTGTTTGATTTTTCATGATGTAGATCACATACCGGAA
AGTGATCGCAACTATTATGGATGTGGACAGATGCCGAGGCATTTTGCAACCAAATTGGAT
AAGTATATGTATCTGCTTCCTTATACCGAGTTCTTTGGCGGAGTGAGTGGCTTAACAGTG
GAACAATTTCGGAAAATCAATGGCTTTCCTAATGCTTTCTGGGGTTGGGGTGGAGAAGAT
GACGACCTCTGGAACAGAGTACAGAATGCAGGCTATTCTGTGAGCCGGCCAGAGGGTGAC
ACAGGAAAGTACAAGTCCATTCCTCATCACCATCGAGGAGAAGTCCAGTTTCTTGGAAGG
TATGCTCTGCTGAGGAAGTCAAAAGAACGGCAAGGGCTGGATGGCCTCAACAACCTGAAC
TACTTTGCAAACATCACATACGACGCCTTGTATAAAAACATAACTGTCAACCTGACACCC
GAGCTGGCTCAGGTGAACGAGTACTGA

Clone variation with respect to NM_004776.3
577 t=>c;660 g=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004776 unedited
TGGATTTATGTATACGACTCACTATATGGGCGGCCGCGATTCGGCACGAGGCGCTTCATG
TGGCCCGGCCCGCGACGGCCGGCGGCTGGGAGCGGCGAGGCGGCGGCGGCGGCGAGTGGC
GGCCCGCGAGGCCCGGGAGGCGGTGGCCGAGGCCCAGGCGGTGGCGGCGGCGGCCCAGGA
GGCGGCGGACGGGGAGCTGCGGGAGCAGGCCCGGCCTGGCTCTCTAGCGGCCGCCTGGCT
GCAGCATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCGCCGCTCGCTGCTCGCCGCGC
TCTTCTTCTTTTCTCTCTCGTCCTCGCTGCTGTACTTCGTCTATGTGGCGCCCGGCATAG
TGAACACCTACCTCTTCATGATGCAAGCCCAAGGCATTCTGATCCGGGACAACGTGAGAA
CAATCGGTGCTCAGGTTTATGAGCAGGTGCTTCGGAGTGCTTATGCCAAGAGGAACAGCA
GTGTAAATGACTCAGATTATCCTCTTGACTTGAACCACAGTGAAACCTTCCTGCANACTA
CAACATTTCTTCCTGAAGACTTCACCTACTTTGCAAACCATACCTGCCCTGAAAGACTCC
CTTCCATGAAGGGCCCAATAGACATAAACATGAGTGAAATTGCGATGGATTACATTCATT
GAACTTTTCTTTCAAGACCCAACCATTCAGCTTCGGAGGTCACTGGGAAGCCTTCTGATT
GCATGCCTTCGTGGAAGGTGGCGATCCTTATTCCCTTCNCGGACCGCCACGAGCACCTCC
CAGTCCTGTTCAGACCCCTGCTTTCCATGCTTCAGCGCCAGCGCCTGCNAGTTGCATTTT
ATGTTGGTTGATCAAGTTTGTTCCCCACCCCTTTAATCGAGGCATGCCTTTTCAACGTNG
GCTTTTCACGAAACATGAAAGAATTGGATAGGGACTTGTTTGATTTTCATGATGTAGATA
CATACCCGCAAGGGATCGCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004776 unedited
GCTATGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTCTTGAAA
TGTATTTTTTACTGAAAAAATCATTCATAAATTAACATACAAAAATGTACAAACACATGA
GTAAATAATGTAATGACAAAGGACTATTTTTGTGAAAAGTGTTTTTTAAAACATCTTTAG
ATTTCAGTGCAAAAATGTACCCCTGGCACCTCTTAAAACGTAAGAGCAAGCTCAAAAACA
CGTAGTGATGGAAATAAGCTAGCTACGCTCAATGCCATCGTCAATGGTGAGTGGATACAA
TCAATCCATGTGGCTCCTGCTCAGCTATTAATGTCTGTGGTAGAGGATCTCTGACAAGAG
GACCTATTATGTCAAAATGAGTATTCTTGCAAATACCCCCCCACCCCCCAAAACAGCATG
AACAAACGGGGACACCTTTCCCTGCACACAGCAATCAAAGACAAGATTAAGGGGCCACCC
ATCAGTAGCCTTGAAGATTTTCAGCTACCAATATATGCACAAGGTCACATTTGGTTCCTG
TTTTGTTTTTTTTTTTAACATGACAATTTCACACTATTAACAGCACACGGGCCTGGCTGT
ACGTTTTTAACTATGACATTTCCCATATGATATTCGAGGCCTGGTGGACACATGACTGAC
AGTGCACATCCAGAGATTCTACACTCATCAGACACAGACATGANCCGCAAGGTATCAAAG
TGANCCCACTTTAGCAGTGAAAAAAAGACCTTTCCTTTTGNTCTCAAATCAGCATTTTCT
AAAGTCAGGTGTTAGCCCTTTGCCAACAGTGTGTACTTGATGGTAATTATGGCACATAGA
GCTTCAACAAGACGNCTGACACACTGCCGATACCATACGGNACCCAGACTCAAATAGTCT
GTCTGCAAATCTTTCATGACTATATGGTGCACATGAGCACTCAACCTCATGAG
Restriction Sites NotI-NotI |
ACCN NM_004776
Insert Size 3780 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004776.2, NP_004767.1
RefSeq Size 4646 bp
RefSeq ORF 1167 bp
Locus ID 9334
UniProt ID O43286
Cytogenetics 20q13.13
Domains Galactosyl_T_2
Protein Families Transmembrane
Protein Pathways O-Glycan biosynthesis
Summary This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. provided by RefSeq, Jul 2008
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"NM_004776" in other vectors (6)
SKU Description Size Price
RC211225 B4GALT5 (Myc-DDK-tagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5) 10 ug
$457.00
RC211225L1 Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5), Myc-DDK-tagged 10 ug
$757.00
RC211225L2 Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5), mGFP tagged 10 ug
$757.00
RC211225L3 Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5), Myc-DDK-tagged 10 ug
$757.00
RC211225L4 Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5), mGFP tagged 10 ug
$757.00
RG211225 B4GALT5 (tGFP-tagged) - Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5) 10 ug
$657.00

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