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COPB2 (NM_004766) Human Untagged Clone

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SKU
SC117165
COPB2 (untagged)-Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1
  $830.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol COPB2
Synonyms beta'-COP; MCPH19
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_004766 edited
GAATTCGGCACGAGGGTCAGTGGATCGGTGGGTTTATCTCAAGGCCTGAGTAGCCGGTAA
CAAACGAGGGTTCCCGGGATTGGACCGACGCAGCCATGCCTCTGCGACTTGATATCAAAA
GAAAGCTAACTGCTAGATCTGATCGAGTTAAGAGTGTGGATCTGCATCCTACAGAGCCAT
GGATGTTGGCAAGTCTTTACAATGGCAGTGTGTGTGTTTGGAATCATGAAACACAGACAC
TGGTGAAGACATTTGAAGTATGTGATCTTCCTGTTCGAGCTGCAAAGTTTGTTGCAAGGA
AGAATTGGGTTGTGACAGGAGCGGATGACATGCAGATTAGAGTGTTCAATTACAATACTC
TGGAGAGAGTTCATATGTTTGAAGCACACTCAGACTACATTCGCTGTATTGCTGTTCATC
CAACCCAGCCTTTCATTCTAACTAGCAGTGATGACATGCTTATTAAGCTCTGGGACTGGG
ATAAAAAATGGTCTTGCTCACAAGTGTTTGAAGGACACACCCATTATGTTATGCAGATTG
TGATCAACCCCAAAGATAACAATCAGTTTGCCAGTGCCTCTTTGGACAGGACTATCAAGG
TGTGGCAGTTGGGCTCTTCGTCACCAAACTTCACTTTGGAAGGACATGAGAAAGGCGTGA
ATTGCATTGATTACTACAGTGGTGGGGACAAGCCATACCTCATTTCAGGTGCAGATGACC
GTCTTGTTAAAATATGGGATTATCAGAATAAAACATGTGTGCAGACACTGGAAGGACATG
CCCAAAATGTGTCTTGTGCCAGCTTTCATCCTGAGTTGCCAATCATTATCACAGGTTCAG
AAGATGGAACAGTACGTATTTGGCATTCAAGCACCTACCGGCTTGAGAGCACACTGAATT
ATGGAATGGAGAGGGTATGGTGCGTGGCCAGTCTAAGAGGGTCAAACAATGTCGCTTTGG
GCTATGATGAAGGGAGCATCATTGTTAAGCTTGGTCGGGAGGAACCTGCCATGTCCATGG
ATGCCAATGGAAAGATAATTTGGGCCAAGCATTCAGAAGTCCAGCAGGCCAACCTAAAAG
CAATGGGAGATGCTGAAATTAAAGATGGTGAAAGATTGCCACTGGCAGTAAAGGATATGG
GCAGTTGTGAAATATACCCTCAGACTATTCAGCACAATCCTAATGGGCGGTTTGTGGTGG
TGTGTGGTGATGGGGAGTATATCATCTACACAGCAATGGCATTGAGAAACAAGAGCTTTG
GATCTGCTCAGGAGTTTGCATGGGCCCACGATTCTTCAGAGTATGCAATAAGAGAGAGCA
ACAGCATTGTAAAGATATTTAAGAACTTTAAGGAAAAAAAATCATTTAAACCAGATTTTG
GAGCAGAAAGTATCTACGGCGGCTTCTTATTGGGAGTCAGATCTGTAAATGGCTTAGCCT
TCTATGACTGGGACAATACAGAACTCATACGAAGAATTGAAATTCAGCCCAAACATATTT
TCTGGTCTGACTCTGGAGAGCTAGTCTGTATTGCTACTGAGGAATCATTTTTTATCCTTA
AGTATCTGTCAGAAAAAGTCTTGGCTGCACAGGAAACACATGAGGGAGTTACTGAAGATG
GCATTGAAGATGCCTTTGAGGTTCTTGGTGAGATTCAGGAAATTGTGAAAACAGGGCTTT
GGGTAGGCGATTGCTTCATTTACACAAGTTCTGTGAACAGATTAAATTATTATGTTGGAG
GAGAAATAGTCACCATTGCCCACTTGGACAGGACGATGTATCTCCTAGGCTACATTCCTA
AAGACAACAGGCTTTATCTGGGGGATAAAGAATTGAACATCATTAGCTATTCCCTGCTGG
TTTCAGTCCTGGAATACCAGACAGCTGTCATGCGGAGGGACTTTAGCATGGCTGATAAGG
TCCTTCCTACCATTCCAAAAGAACAGAGGACCAGAGTTGCACACTTTTTGGAAAAGCAGG
GCTTCAAGCAGCAAGCTCTTACAGTATCCACAGATCCTGAGCATCGTTTTGAGCTTGCTC
TTCAGCTTGGAGAGTTAAAAATTGCATACCAGTTAGCAGTGGAAGCAGAGTCAGAACAGA
AGTGGAAACAACTTGCTGAACTTGCCATTAGTAAATGTCAGTTTGGCCTAGCCCAGGAGT
GCCTGCATCATGCACAGGATTATGGGGGCCTGCTGCTTTTGGCCACTGCCTCTGGAAATG
CTAATATGGTGAACAAGCTAGCAGAGGGTGCGGAGAGAGATGGCAAAAATAATGTGGCAT
TCATGAGCTACTTTTTACAGGGCAAGGTTGATGCCTGCCTAGAGCTCTTAATTAGAACTG
GACGGCTGCCAGAAGCTGCCTTCTTGGCCCGAACTTACTTACCCAGTCAGGTTTCAAGGG
TAGTGAAACTCTGGAGAGAGAATCTCTCAAAAGTCAATCAGAAAGCAGCAGAATCCCTTG
CTGACCCAACAGAGTATGAAAACCTGTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAG
AATGGGTGAAGGAAACACATGCTGATCTGTGGCCAGCCAAACAATACCCACTTGTCACGC
CAAATGAAGAGAGAAATGTCATGGAAGAGGGAAAAGACTTTCAGCCCTCAAGATCTACAG
CTCAACAGGAACTTGATGGGAAACCTGCTTCTCCTACTCCGGTTATTGTGGCCTCCCACA
CAGCCAACAAAGAAGAAAAGAGTTTACTCGAACTAGAAGTAGATTTGGATAATTTGGAAT
TAGAAGATATTGACACAACAGATATCAATCTGGATGAAGATATTTTGGATGATTGACTGT
AATGCTTTCCATTTACCTGACTAAACAGATCATTATTATATATAGGTATTGATTGCTACC
CTGACCACAGTGCTTTGGACTATGAGAAACTTCTTAGATTTTTATATGTAAATGCTGTGG
ACCACTGGGAGCACAATGCCCACATCATCTTAAGAAGAGTTTATGTGCAGCATTTAAATC
ACTGTGTTTTCCTTGTTAACTAAAACAGACATGGGCTTTGATTTTTTTCATACTATTAGA
CCATATCTCATAAAACCTTTTGAATTAATGAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004766 unedited
ATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGTCAGTGGATCGGTGGG
TTTATCTCAAGGCCTGAGTAGCCGGTAACAAACGAGGGTTCCCGGGATTGGACCGACGCA
GCCATGCCTCTGCGACTTGATATCAAAAGAAAGCTAACTGCTAGATCTGATCGAGTTAAG
AGTGTGGATCTGCATCCTACAGAGCCATGGATGTTGGCAAGTCTTTACAATGGCAGTGTG
TGTGTTTGGAATCATGAAACACAGACACTGGTGAAGACATTTGAAGTATGTGATCTTCCT
GTTCGAGCTGCAAAGTTTGTTGCAAGGAAGAATTGGGTTGTGACAGGAGCGGATGACATG
CAGATTAGAGTGTTCAATTACAATACTCTGGAGAGAGTTCATATGTTTGAAGCACACTCA
GACTACATTCGCTGTATTGCTGTTCATCCAACCCAGCCTTTCATTCTAACTAGCAGTGAT
GACATGCTTATTAAGCTCTGGGACTGGGATAAAAAATGGTCTTGCTCACAAGTGTTTGAA
GGACACACCCATTATGTTATGCAGATTGTGATCAACCCCAAAGATAACAATCAGTTTGCC
AGTGCCTCTTTGGACAGGACTATCAAGGTGTGGCAGTTGGGCTCTTCGTCACCAAACTTC
ACTTTGGAAGGACATGAGAAAGGCGTGAATTGCATTGATTACTACAGTGGTGGGGACAAG
CCATACCTCATTTCAGGTGCAGATGACCGTCTTTGTTAAATATGGGATTATCAGAATANA
ACATGTGTGCAGACACTGGAAGGACATGCCCAAAATGTGTCTTGCGGCAGCTTTCATCCT
GAGTTGCCAATCATATCACAGGTTCAGAAGATGGACAGTACGTATTTGCATTCAGCACCT
ACCGGCTGAGAGCACACTGATATGNATGGANAGGTATGTGCGTGCCAGCTAAAGTTCAAC
ATGTCGCTTGGCTTGATGAGGACTCATGTAGCTGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004766 unedited
NCCTTCTTGNACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCATTAATT
CAAAGGTTTTATGAGATATGGTCTAATAGTATGAAAAAATCAAAGCCCATGTCTGTTTTA
GTTAACAAGGAAAACACAGTGATTTAAATGCTGCACATAAACTCTTCTTAAGATGATGTG
GGCATTGTGCTCCCAGTGGTCCACAGCATTTACATATAAAAATCTAAGAAGTTTCTCATA
GTCCAAAGCACTGTGGTCAGGGTAGCAATCAATACCTATATATAATAATGATCTGTTTAG
TCAGGTAAATGGAAAGCATTACAGTCAATCATCCAAAATATCTTCATCCAGATTGATATC
TGTTGTGTCAATATCTTCTAATTCCAAATTATCCAAATCTACTTCTAGTTCGAGTAAACT
CTTTTCTTCTTTGTTGGCTGTGTGGGAGGCCACAATAACCGGAGTAGGAGAAGCAGGTTT
CCCATCAAGTTCCTGTTGAGCTGTAGATCTTGAGGGCTGAAAGTCTTTTCCCTCTTCCAT
GACATTTCTCTCTTCATTTGGCGTGACAAGTGGGTATTGTTTGGCTGGCCACAGATCAGC
ATGTGTTTCCTTCACCCATTCTTCAACAACAAAGGCTTCTTTTAATCCAGGGAACAGGTT
TTCATACTCTGTTGGGTCAGCAAGGGATTCTGCTGCTTTCTGATTGACTTTTGAGAGATT
CTCTCTCCAGAGTTTCACTACCCTTGAAACCTGACTGGGTAAGTAAAGTCGGGCCAAGAA
GCAGCTTCTGGCAGCCGTCCAGTTCTATTAAGAGCTCTNAGCAGGCATCAACCTTGCCCT
GTAAAAGTAGCTCATGAATGCCACATTNATTTTGCCATCTCTCTCCGNACCCTCTGCTAG
CTTGTTCCACATATTAGCATTTCCANAGGCAGTGGCCAAAAA
Restriction Sites NotI-NotI |
ACCN NM_004766
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004766.1, NP_004757.1
RefSeq Size 3067 bp
RefSeq ORF 2721 bp
Locus ID 9276
UniProt ID P35606
Cytogenetics 3q23
Domains Coatomer_WDAD, WD40
Protein Families Druggable Genome
Summary The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.supplied by OMIM, Jul 2002
Transcript Variant: This variant (1) uses an alternate splice donor site in the first coding exon and is predicted to encode a protein.
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Documents
Resources
"NM_004766" in other vectors (6)
SKU Description Size Price
RC201789 COPB2 (Myc-DDK-tagged)-Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1 10 ug
$829.00
RC201789L1 Lenti ORF clone of Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,129.00
RC201789L2 Lenti ORF clone of Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1, mGFP tagged 10 ug
$1,129.00
RC201789L3 Lenti ORF clone of Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,129.00
RC201789L4 Lenti ORF clone of Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1, mGFP tagged 10 ug
$1,129.00
RG201789 COPB2 (tGFP-tagged) - Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1 10 ug
$789.00 $1,029.00

file-alt Citations

COPB2 promotes metastasis and inhibits apoptosis of lung adenocarcinoma cells through functioning as a target of miR-216a-3p
Wang, Yu, Ling et al
Transl Cancer Res (2020) 9 (4), 2648-2659 DOI: 10.21037/tcr.2020.02.65
Cited by 6
AI Summary: In this study, OriGene products including COPB2 siRNA (SR307372) and COPB2 antibody (TA500439) were used to specifically silence and detect COPB2 expression, enabling the authors to investigate the role of COPB2 in lung adenocarcinoma cell metastasis and apoptosis.
FigureFigureFigure
COPB2 promotes cell proliferation and tumorigenesis through up-regulating YAP1 expression in lung adenocarcinoma cells
Pu, Wang, Li et al
Biomed. Pharmacother. (2018) 103, 373-380 DOI: 10.1016/j.biopha.2018.04.006
AI Summary: OriGene products, including COPB2 siRNA (SR306142), YAP1 siRNA (SR323110), and empty vector controls, were used to modulate gene expression and serve as experimental controls in investigating the roles of COPB2 and YAP1 in lung adenocarcinoma cell proliferation and tumorigenesis.
Snippet: siRNA (No. SR306142) and YAP1 (No. SR323110)were purchased from OriGene (MA, USA). The empty vector obtained from OriGene (MA, USA) was used as the negative control of COPB2 and YAP1 plasmids. The cells were
A conserved motif in transmembrane helix 1 of diphtheria toxin mediates catalytic domain delivery to the cytosol
Ratts, Trujillo, Bharti et al
Proc Natl Acad Sci U S A (2005) 102 (43), 15635-40 DOI: 10.1073/pnas.0504937102
Cited by 50
AI Summary: OriGene products contributed to this study by providing the CMV promoter-driven expression plasmid used to generate the T1 peptide-expressing Hut102/6TG-T1 cell line (OriGene product: pCMV6 vector), enabling functional analysis of the conserved motif in diphtheria toxin transmembrane helix 1.
Showing 1-3 of 3 papers.
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