TBPL1 (NM_004865) Human Untagged Clone

SKU
SC117107
TBPL1 (untagged)-Human TBP-like 1 (TBPL1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TBPL1
Synonyms MGC:8389; MGC:9620; STUD; TLF; TLP; TRF2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117107 sequence for NM_004865 edited (data generated by NextGen Sequencing)
ATGGATGCAGACAGTGATGTTGCATTGGACATTCTAATTACAAATGTAGTCTGTGTTTTT
AGAACAAGATGTCATTTAAACTTAAGGAAGATTGCTTTGGAAGGAGCAAATGTAATTTAT
AAACGTGATGTTGGAAAAGTATTAATGAAGCTTAGAAAACCTAGAATTACAGCTACAATT
TGGTCCTCAGGAAAAATTATTTGCACTGGAGCAACAAGTGAAGAAGAAGCTAAATTTGGT
GCCAGACGCTTAGCCCGTAGTCTGCAGAAACTAGGTTTTCAGGTAATATTTACAGATTTT
AAGGTTGTTAACGTTCTGGCAGTGTGTAACATGCCATTTGAAATCCGTTTGCCAGAATTC
ACAAAGAACAATAGACCTCATGCCAGTTACGAACCTGAACTTCATCCTGCTGTGTGCTAT
CGGATAAAATCTCTAAGAGCTACATTACAGATTTTTTCAACAGGAAGTATCACAGTAACA
GGGCCCAATGTAAAGGCTGTTGCTACTGCTGTGGAACAGATTTACCCATTTGTGTTTGAA
AGCAGGAAAGAAATTTTATAA

Clone variation with respect to NM_004865.2
309 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004865 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGAGCGCGCCCGT
CTCATCCTCATCCTTGTCCTCCAGCTTCTCCTTCGCATCCGACGCAACCGGCAGCAGCGC
TGCCGCCGCGTCCTCAGCCACCGCTCCCTCTTCCCACGGATGTGATCTTCGTGGTGGAAA
GCTAAATTTTAAAACCACCCCAATGGATGCAGACAGTGATGTTGCATTGGACATTCTAAT
TACAAATGTAGTCTGTGTTTTTAGAACAAGATGTCATTTAAACTTAAGGAAGATTGCTTT
GGAAGGAGCAAATGTAATTTATAAACGTGATGTTGGAAAAGTATTAATGAAGCTTAGAAA
ACCTAGAATTACAGCTACAATTTGGTCCTCAGGAAAAATTATTTGCACTGGAGCAACAAG
TGAAGAAGAAGCTAAATTTGGTGCCAGACGCTTAGCCCGTAGTCTGCAGAAACTAGGTTT
TCAGGTAATATTTACAGATTTTAAGGTTGTTAACGTTCTGGCAGTGTGTAACATGCCATT
TGAAATCCGTTTGCCAGAATTCACAAAGAACAATAGACCTCATGCCAGTTACGAACCTGA
ACTTCATCCTGCTGTGTGCTATCGGATAAAATCTCTAAGAGCTACATTACAGATTTTTTC
AACAGGAAGTATCACAGTAACAGGGCCCAATGTAAAGGCTGTTGCTACTGCTGTGGAACA
GATTTACCCATTTTGTGTTTGAAAGCAGGAAAGATATTNTATNATTCACCACTTAATTGG
TTAGAATCTCTAACTGAGCACTTTTAACCTGCTGACATTGGACTCAAAGGAANACTGGAC
AACNNATATGAGAANTGACTCTTTATTCATTCACGCTACGTGTAGCTTCCAGTCCTTA
Restriction Sites NotI-NotI |
ACCN NM_004865
Insert Size 2930 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004865.2, NP_004856.1
RefSeq Size 1340 bp
RefSeq ORF 561 bp
Locus ID 9519
UniProt ID P62380
Cytogenetics 6q23.2
Domains TBP
Protein Families Transcription Factors
Protein Pathways Basal transcription factors, Huntington's disease
Summary This gene encodes a member of the TATA box-binding protein family. TATA box-binding proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA box and initiates transcription from TATA-less promoters. This gene plays a critical role in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. provided by RefSeq, Nov 2011
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_004865" in other vectors (4)
SKU Description Size Price
RC201750 TBPL1 (Myc-DDK-tagged)-Human TBP-like 1 (TBPL1) 10 ug
$300.00
RC201750L3 Lenti ORF clone of Human TBP-like 1 (TBPL1), Myc-DDK-tagged 10 ug
$600.00
RC201750L4 Lenti ORF clone of Human TBP-like 1 (TBPL1), mGFP tagged 10 ug
$600.00
RG201750 TBPL1 (tGFP-tagged) - Human TBP-like 1 (TBPL1) 10 ug
$489.00 $500.00

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