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CLOCK (NM_004898) Human Untagged Clone

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SKU
SC117088
CLOCK (untagged)-Human clock homolog (mouse) (CLOCK)
  $776.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CLOCK
Synonyms bHLHe8; KAT13D
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117088 sequence for NM_004898 edited (data generated by NextGen Sequencing)
ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGT
ATTTTTGATGGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAAC
AAATCTGAAAAGAAACGTAGAGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATG
CTTCCTGGTAATGCTAGAAAGATGGACAAATCTACTGTTCTGCAGAAAAGCATTGATTTT
TTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTAGTGAAATTCGACAGGACTGG
AAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGCTCTTGATGGT
TTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTAACTTCA
TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAA
GGGGAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTA
ACCCCAGAATATTTAAAATCAAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGA
ACAATAGACCCAAAGGAGCCATCTACCTATGAATATGTAAAATTTATAGGAAATTTCAAA
TCTTTAAACAGTGTATCCTCTTCAGCACACAATGGTTTTGAAGGAACTATACAACGCACA
CATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGGTTAGCTACACCT
CAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTTACATCTAGACAT
AGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTATTTG
CCATTTGAAGTTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAAT
TTGGCAAAATGTCATGAGCACTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGG
TTCCTGACTAAGGGGCAACAGTGGATTTGGCTTCAGACTCATTATTATATCACTTACCAT
CAGTGGAATTCAAGGCCAGAGTTTATTGTTTGTACTCACACTGTAGTAAGTTATGCAGAA
GTTAGGGCTGAAAGACGACGAGAACTTGGCATTGAAGAGTCTCTTCCTGAGACAGCTGCT
GACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACAGTCAGTCTCAAGGAAGCA
TTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCAAGAAAATCA
TCTCACACGGCCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGC
ACTCCACCCAGGCAGCATTTACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTT
AGTAGTCAGTCCATAAATTCCCAGTCTGTTGGTTCATCATTAACACAGCCAGTGATGTCT
CAAGCTACAAATTTACCAATTCCACAAGGCATGTCCCAGTTTCAGTTTTCAGCTCAATTA
GGAGCCATGCAACATCTGAAAGACCAATTGGAACAACGGACACGCATGATAGAAGCAAAT
ATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAAGAACAACTTCAGATGGTCCATGGT
CAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAATTTTGGTTCCGTTCAA
CTTTCTTCTGGAAATTCATCTAACATCCAGCAACTTGCACCTATAAATATGCAAGGCCAA
GTTGTTCCTACTAACCAGATTCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAG
CACATGATACAACAACAGACTTTACAGAGTACATCAACTCAGAGTCAACAAAATGTACTG
AGTGGGCACAGTCAGCAAACATCTCTACCCAGTCAGACACAGAGCACTCTTACAGCCCCA
CTGTATAACACTATGGTGATTTCTCAGCCTGCAGCCGGAAGCATGGTCCAGATTCCATCT
AGTATGCCACAAAACAGCACCCAGAGTGCTGCAGTAACTACATTCACTCAGGACAGGCAG
ATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGT
GGGGCAGTCATGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACTGCATATCCTACT
TTTGCTACACAACAGCAACAGTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAGCAG
AGCTCCCAGGAGCAGCAGCTCACTTCAGTTCAGCAACCATCTCAGGCTCAGCTGACCCAG
CCACCGCAACAATTTTTACAGACTTCTAGGTTGCTCCATGGGAATCCCTCAACTCAACTC
ATTCTCTCTGCTGCATTTCCTCTACAACAGAGCACCTTCCCTCAGTCACATCACCAGCAA
CATCAGTCTCAGCAACAGCAGCAACTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCT
TCCAAGGTTCAACCACAGTAG

Clone variation with respect to NM_004898.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004898 unedited
NNTGGTTCGNATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGCACCGC
AGCGGGAGACCTGGGTGGGGGAGGGAAGAAGCCGGAGCCGCCGCAAGCCACACGAATTCT
GAGACTTATGGTTGGTCATATAGAAGAGTACCTTGAACCTATAGTTTCCTGAAGAATCAG
TTTAAAAGATCCAAGGAGTACAAAAGGAGAAGTACAAATGTCTACTACAAGACGAAAACG
TAGTATGTTATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGAT
GACAGTAGTATTTTTGATGGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTA
TCTAGAAACAAATCTGAAAAGAAACGTAGAGATCAATTTAATGTTCTCATTAAAGAACTG
GGATCCATGCTTCCTGGTAATGCTAGAAAGATGGACAAATCTACTGTTCTGCAGAAAAGC
ATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTAGTGAAATTCGA
CAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGCT
CTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGT
GTAACTTCATTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTT
ATCCCAGAAGGGGAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGT
GATTCATTAACCCCAGAATATTTNAAAATCAAAAATCAGTTAGAATTCTGTTGTCACATG
CTGCGAGGNAACATAGACCCAAAGGAGCCATCTACCTATGAATATGNNTAAATTATAGGN
AAATTNCANATCTTTAACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004898 unedited
AGCTTGGACCGCGCTCCAATTTANGATCGGTTTTTTTTCTTTTTTTTTTTAAAGCTGGAA
GGTGTTTCTATTTGGTTTCTTTAAATGCTCTAGATTTGGGATTTTAACTAACAGCTTTGG
CTATAGAAATGAAGAACAGACACTGCTGAATCTTCATTTGAATGCTCAAGTTTCATGGGG
CAGGGTGGGGGGCGGGATCGCTTTGCAACCCATTTTTTTGAGAATGCGGAGGAAAACATT
TCTTCCAGAGGCCAGTGGTGCTAGCTACCTCGGCCCGTGCCCGCGTGCGCGCTGCCCCGT
CTCTGCGTACCCTGTTCTTTCTCCTCACTCCCCCTTGCACCCTCCTCTCCCCCCCCTCCC
CCCCCCCCCCCCGTCCCCCCCCCCCCCCCCCCCGCCCTCCCACCTCCCCCGCTCTGTCTT
TCCCTCGCCTTGACCCTCCTTTCCAATCTTCCCTCCGTATTTCCCTCCCCCACCCCCCCC
CTGCTCCCCTCCTCGCCTCCCCGTCTCTCCCCTCCCCGTCCGCTCCCCCCCTCCCCCTCC
TTGGCCCCCCGTCCCCTGTCTCCTCCGCCCTCGCCTAGTGCTCTCCCGCCGCCTCCCGCC
CCCCCCCCCCCCCCCCCCCCCCCCCACGCGTCCTTCGGGGTGGCCTTTCTCCCCCGTCCC
GCTCTATCCCACCCCTCCCCCGTCCTTGCCGCCCTCCTGCTCCGCTTCCTCGCCGGCCCC
CCTTACCTCCTCGCCCTGCGCCGCCCCCGCCCCCTCCCCCCCCCCGTTGCCCCCGCCCCC
GCCCCATGCCCGTGCCGTCCCGCCAGCTTTCCTTTCACCGATCCCCCCCCCACGTACGTG
TCGCGCGTCGCCTGCCGCAGCCCCCGAGCGCCTCCCTATCTACTTCACCCCGCGCCCCCC
TCCCCACACCCCCCCCGACC
Restriction Sites NotI-NotI |
ACCN NM_004898
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004898.2, NP_004889.1
RefSeq Size 5801 bp
RefSeq ORF 2541 bp
Locus ID 9575
UniProt ID O15516
Cytogenetics 4q12
Domains HLH, PAC, PAS
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Circadian rhythm - mammal
Summary The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2014
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_004898" in other vectors (6)
SKU Description Size Price
RC221408 CLOCK (Myc-DDK-tagged)-Human clock homolog (mouse) (CLOCK) 10 ug
$775.00
RC221408L1 Lenti ORF clone of Human clock homolog (mouse) (CLOCK), Myc-DDK-tagged 10 ug
$1,075.00
RC221408L2 Lenti ORF clone of Human clock homolog (mouse) (CLOCK), mGFP tagged 10 ug
$1,075.00
RC221408L3 Lenti ORF clone of Human clock homolog (mouse) (CLOCK), Myc-DDK-tagged 10 ug
$1,075.00
RC221408L4 Lenti ORF clone of Human clock homolog (mouse) (CLOCK), mGFP tagged 10 ug
$1,075.00
RG221408 CLOCK (tGFP-tagged) - Human clock homolog (mouse) (CLOCK) 10 ug
$975.00

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