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Calbindin (CALB1) (NM_004929) Human Untagged Clone

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SKU
SC117035
CALB1 (untagged)-Human calbindin 1, 28kDa (CALB1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Calbindin
Synonyms CALB; D-28K
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117035 sequence for NM_004929 edited (data generated by NextGen Sequencing)
ATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGATCTGG
CTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATC
CAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAAAACT
TTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGCTCAC
GTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTCCTGT
GAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGAAACT
GAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGATGAC
ACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGATGGG
AAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAAA
TTCCAGGGAATCAAAATGTGTGGGAAAGAGTTCAATAAGGCTTTTGAGCTGTATGATCAG
GACGGCAATGGATACATAGATGAAAATGAACTGGATGCTTTACTGAAGGATCTGTGCGAG
AAGAATAAACAGGATCTGGATATTAATAATATTACAACATACAAGAAGAACATAATGGCT
TTGTCGGATGGAGGGAAGCTGTACCGAACGGATCTTGCTCTTATTCTCTGTGCTGGGGAT
AACTAG

Clone variation with respect to NM_004929.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004929 unedited
GCGGCACGCGCAATTCGGCACGAGGCTGAGCTGAGCAGCACCGAGGACAGCGCCCGGCAG
CGCCCGCGCCCAGGTCTCCCTCCGCAGCCCTGACTCGCGCACACGCTGAGCTTTTGCTCA
CTCCCCCTCGCGCGGACACAGACACACGCATATTCACACACCCAGACACACACCCCGCTG
TACAATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGAT
CTGGCTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTT
GATCCAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAA
AACTTTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGC
TCACGTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTC
CTGTGAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGA
AACTGAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGA
TGACACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGA
TGGGAAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCT
TAATTCCAGGGAATCAAAATGTGTGGGAAAAGAGTTCATAAGGGCTTTGAGCTGTATGAT
CAGGACNNGCATGGATACATAGATGAAAATGAACTGGGATGCTTACTGAAGGATCTGTGC
GAGAAGAATAACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004929 unedited
GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTAAGGTTATTTTCT
TCTTTATTTATTCTTAGTAGTTAAATTCAGTCTAATGGCAGTGCTTCCATTTACACGCTA
AAGGCAAAGCCAATCTCCCACTCAACATCATTTGTTGAATTTTAGTTTTTGGCAAATCTC
GTTTCTGTTTGTTCTTGAGGGAAACATTATAGCTCACATTTTTTGGTTATTTTTATGAAT
TCAGAAAATAAGTGTTGAATTTATAGAATGGGGACATTCAAGCTGACCCAGAACGATCAT
TAACTCCTAGGCAGTAACTGTAAATTTCACATGGACACATCTCTCATAGCTCCCTGTTTC
ACAATCAAAGAAATCTAAAATCACCAACTGTTACATATCAACTATAACCAGTATAGGATA
TTCTAGCTACAAATATGTGAAACTGACTTAGATGTATGTTAAAGAATGTTTTAGAATCTT
CTTGCTAGATAAGATAAATGATTACATTAGCATTTCTTTTCAGATGTTATTTTTTTAAAC
TCATTTGAAACTATTTGCAGATAGAAATAGAACTATTTTGAATGCTGATCTCAATATGAA
ATATAGAATCCACAGAATACACAAAAGTATTGATGATATAACATTCAATAATCAGGATGC
TATGAATTTTACATTAAAATTTAATTATGTAGTAAAAAATANGAGTTGTTATAGCTAGAA
AAAAATATTTTCAGAGGCAGCAGATACCCTTGGTGGAANTGAACANGTTCTCATAGAAAG
GAAATCCCAGAAGTATTGCAACTAGACACAGGATATGCAGTATATAAAATTAATCAGAGA
TACACAGGTTTGACATAAACAGCNGGTGACTGAATATTTTGCACACTGAAAACAGTTAGA
AATGGATTAGGTCCTATCTCTGACGAAAATTGTG
Restriction Sites NotI-NotI |
ACCN NM_004929
Insert Size 2660 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004929.2, NP_004920.1
RefSeq Size 2531 bp
RefSeq ORF 786 bp
Locus ID 793
UniProt ID P05937
Cytogenetics 8q21.3
Domains EFh
Summary The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. provided by RefSeq, Jan 2015
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Documents
Resources
"NM_004929" in other vectors (6)
SKU Description Size Price
RC201358 CALB1 (Myc-DDK-tagged)-Human calbindin 1, 28kDa (CALB1) 10 ug
$300.00
RC201358L1 Lenti ORF clone of Human calbindin 1, 28kDa (CALB1), Myc-DDK-tagged 10 ug
$600.00
RC201358L2 Lenti ORF clone of Human calbindin 1, 28kDa (CALB1), mGFP tagged 10 ug
$600.00
RC201358L3 Lenti ORF clone of Human calbindin 1, 28kDa (CALB1), Myc-DDK-tagged 10 ug
$600.00
RC201358L4 Lenti ORF clone of Human calbindin 1, 28kDa (CALB1), mGFP tagged 10 ug
$600.00
RG201358 CALB1 (tGFP-tagged) - Human calbindin 1, 28kDa (CALB1) 10 ug
$489.00 $500.00

file-alt Citations

Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans
Schumacher, Hsieh, Chen et al
Development (2012) 139 (22), 4191-201 DOI: 10.1242/dev.083428
Cited by 49
AI Summary: OriGene’s pCMV6-calbindin-28K plasmid was used as the source of M. musculus calbindin D28K cDNA for generating transgenic constructs to manipulate intracellular calcium levels and investigate the role of calcium signaling in neuronal fate determination in C. elegans.
Snippet: M. musculus calbindin D28K cDNA from pCMV6-calbindin-28K (Origene) was subcloned to make nsy-5p::calbindin D28K, odr-3p::calbindin D28K, nsy-5p:calbindin D28K::mCherry, str-1p::calbindin D28K and sra-6p::calbindin D28K::SL2::mCherry.
Showing 1-1 of 1 papers.
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