MVP (NM_005115) Human Untagged Clone

SKU SC116936

MVP (untagged)-Human major vault protein (MVP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MVP
• Synonyms: LRP; VAULT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005115 edited; GAATTCGGCACGAGGGTTTGTTGCAGCTACCTGCACTTCTAGATCCCTGGGCTTAGGAGT; CACCATGGCAACTGAAGAGTTCATCATCCGCATCCCCCCATACCACTATATCCATGTGCT; GGACCAGAACAGCAACGTGTCCCGTGTGGAGGTCGGGCCAAAGACCTACATCCGGCAGGA; CAATGAGAGGGTACTGTTTGCCCCCATGCGCATGGTGACCGTCCCCCCACGTCACTACTG; CACAGTGGCCAACCCTGTGTCTCGGGATGCCCAGGGCTTGGTGCTGTTTGATGTCACAGG; GCAAGTTCGGCTTCGCCACGCTGACCTCGAGATCCGGCTGGCCCAGGACCCCTTCCCCCT; GTACCCAGGGGAGGTGCTGGAAAAGGACATCACACCCCTGCAGGTGGTTCTGCCCAACAC; TGCCCTCCATCTAAAGGCGCTGCTTGATTTTGAGGATAAAGATGGAGACAAGGTGGTGGC; AGGAGATGAGTGGCTTTTCGAGGGACCTGGCACGTACATCCCCCGGAAGGAAGTGGAGGT; CGTGGAGATCATTCAGGCCACCATCATCAGGCAGAACCAGGCTCTGCGGCTCAGGGCCCG; CAAGGAGTGCTGGGACCGGGACGGCAAGGAGAGGGTGACAGGGGAAGAATGGCTGGTCAC; CACAGTAGGGGCGTACCTCCCAGCGGTGTTTGAGGAGGTTCTGGATTTGGTGGACGCCGT; CATCCTTACGGAAAAGACAGCCCTGCACCTCCGGGCTCGGCGGAACTTCCGGGACTTCAG; GGGAGTGTCCCGCCGCACTGGGGAGGAGTGGCTGGTAACAGTGCAGGACACAGAGGCCCA; CGTGCCAGATGTCCACGAGGAGGTGCTGGGGGTTGTGCCCATCACCACCCTGGGCCCCCA; CAACTACTGCGTGATTCTCGACCCTGTCGGACCGGATGGCAAGAATCAGCTGGGGCAGAA; GCGCGTGGTCAAGGGAGAGAAGTCTTTTTTCCTCCAGCCAGGAGAGCAGCTGGAACAAGG; CATCCAGGATGTGTATGTGCTGTCGGAGCAGCAGGGGCTGCTGCTGAGGGCCCTGCAGCC; CCTGGAGGAGGGGGAGGATGAGGAGAAGGTCTCACACCAGGCTGGGGACCACTGGCTCAT; CCGCGGACCCCTGGAGTATGTGCCATCTGCCAAAGTGGAGGTGGTGGAGGAGCGCCAGGC; CATCCCTCTAGACGAGAACGAGGGCATCTATGTGCAGGATGTCAAGACCGGAAAGGTGCG; CGCTGTGATTGGAAGCACCTACATGCTGACCCAGGACGAAGTCCTGTGGGAGAAAGAGCT; GCCTCCCGGGGTGGAGGAGCTGCTGAACAAGGGGCAGGACCCTCTGGCAGACAGGGGTGA; GAAGGACACAGCTAAGAGCCTCCAGCCCTTGGCGCCCCGGAACAAGACCCGTGTGGTCAG; CTACCGCGTGCCCCACAACGCTGCGGTGCAGGTGTACGACTACCGAGAGAAGCGAGCCCG; CGTGGTCTTCGGGCCTGAGCTGGTGTCGCTGGGTCCTGAGGAGCAGTTCACAGTGTTGTC; CCTCTCAGCTGGGCGGCCCAAGCGTCCCCATGCCCGCCGTGCGCTCTGCCTGCTGCTGGG; GCCTGACTTCTTCACAGACGTCATCACCATCGAAACGGCGGATCATGCCAGGCTGCAACT; GCAGCTGGCCTACAACTGGCACTTTGAGGTGAATGACCGGAAGGACCCCCAAGAGACGGC; CAAGCTCTTTTCAGTGCCAGACTTTGTAGGTGATGCCTGCAAAGCCATCGCATCCCGGGT; GCGGGGGGCCGTGGCCTCTGTCACTTTCGATGACTTCCATAAGAACTCAGCCCGCATCAT; TCGCACTGCTGTCTTTGGCTTTGAGACCTCGGAAGCGAAGGGCCCCGATGGCATGGCCCT; GCCCAGGCCCCGGGACCAGGCTGTCTTCCCCCAAAACGGGCTGGTGGTCAGCAGTGTGGA; CGTGCAGTCAGTGGAGCCTGTGGATCAGAGGACCCGGGACGCCCTGCAACGCAGCGTCCA; GCTGGCCATCGAGATCACCACCAACTCCCAGGAAGCGGCGGCCAAGCATGAGGCTCAGAG; ACTGGAGCAGGAAGCCCGCGGCCGGCTTGAGCGGCAGAAGATCCTGGACCAGTCAGAAGC; CGAGAAAGCTCGCAAGGAACTTTTGGAGCTGGAGGCTCTGAGCATGGCCGTGGAGAGCAC; CGGGACTGCCAAGGCGGAGGCCGAGTCCCGTGCGGAGGCAGCCCGGATTGAGGGAGAAGG; GTCCGTGCTGCAGGCCAAGCTAAAAGCACAGGCCTTGGCCATTGAAACGGAGGCTGAGCT; CCAGAGGGTCCAGAAGGTCCGAGAGCTGGAACTGGTCTATGCCCGGGCCCAGCTGGAGCT; GGAGGTGAGCAAGGCTCAGCAGCTGGCTGAGGTGGAGGTGAAGAAGTTCAAGCAGATGAC; AGAGGCCATAGGCCCCAGCACCATCAGGGACCTTGCTGTGGCTGGGCCTGAGATGCAGGT; AAAACTGCTCCAGTCCCTGGGCCTGAAATCAACCCTCATCACCGATGGCTCCACTCCCAT; CAACCTCTTCAACACAGCCTTTGGGCTGCTGGGGATGGGGCCCGAGGGTCAGCCCCTGGG; CAGAAGGGTGGCCAGTGGGCCCAGCCCTGGGGAGGGGATATCCCCCCAGTCTGCTCAGGC; CCCTCAAGCTCCTGGAGACAACCACGTGGTGCCTGTACTGCGCTAACTCCTGATTAATAC; AATGGAAGTTTCTGGGCATTTACAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005115 unedited; CAGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACAGGGGTTTGTTGCAGCT; ACCTGCACTTCTAGATCCCTGGGCTTAGGAGTCACCATGGCAACTGAAGAGTTCATCATC; CGCATCCCCCCATACCACTATATCCATGTGCTGGACCAGAACAGCAACGTGTCCCGTGTG; GAGGTCGGGCCAAAGACCTACATCCGGCAGGACAATGAGAGGGTACTGTTTGCCCCCATG; CGCATGGTGACCGTCCCCCCACGTCACTACTGCACAGTGGCCAACCCTGTGTCTCGGGAT; GCCCAGGGCTTGGTGCTGTTTGATGTCACAGGGCAAGTTCGGCTTCGCCACGCTGACCTC; GAGATCCGGCTGGCCCAGGACCCCTTCCCCCTGTACCCAGGGGAGGTGCTGGAAAAGGAC; ATCACACCCCTGCAGGTGGTTCTGCCCAACACTGCCCTCCATCTAAAGGCGCTGCTTGAT; TTTGAGGATAAAGATGGAGACAAGGTGGTGGCAGGAGATGAGTGGCTTTTCGAGGGACCT; GGCACGTACATCCCCCGGAAGGAAGTGGAGGTCGTGGAGATCATTCAGGCCACCATCATC; AGGCAGAACCAGGCTCTGCGGCTCAGGGCCCGCAAGGAGTGCTGGGACCGGGACGGCAAG; GAGAGGGTGACAGGGGAAGAATGGCTGGTCACCACAGTAGGGGCGTACCTCCCAGCGGTG; TTTGAGGAGGNTCTGGATTTGGTGGACGCCGTCATCCTTACGGAAAAGAAGCCCTGCACC; TCCGGGCTCGGCGGAACTTCCGGGACTTCAGGGGAGTGTCCCGCCGCACTGGNGNAGAGT; GGGCTGGTACAGTGCANGACACAGAAGGCCACGTGCCANAAGTNCACAAGGAGTGCTGGG; GGGGTTGTTGCCATTACCACCCTGGGCCCCNACAACTACTGCGTGAATCTCGACCCTGTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005115 unedited; GCCGCACTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGTAAATGCCCAAAAACTTCCATT; GTATTAATCAGGAGTTAGCGCAGTACAGGCACCACGTGGTTGTCTCCAGGAGCTTGAGGG; GCCTGAGCAGACTGGGGGGATATCCCCTCCCCAGGGCTGGGCCCACTGGCCACCCTTCTG; CCCAGGGGCTGACCCTCGGGCCCCATCCCCAGCAGCCCAAAGGCTGTGTTGAAGAGGTTG; ATGGGAGTGGAGCCATCGGTGATGAGGGTTGATTTCAGGCCCAGGGACTGGAGCAGTTTT; ACCTGCATCTCAGGCCCAGCCACAGCAAGGTCCCTGATGGTGCTGGGGCCTATGGCCTCT; GTCATCTGCTTGAACTTCTTCACCTCCACCTCAGCCAGCTGCTGAGCCTTGCTCACCTCC; AGCTCCAGCTGGGCCCGGGCATAGACCAGTTCCAGCTCTCGGACCTTCTGGACCCTCTGG; AGCTCAGCCTCCGTTTCAATGGCCAAGGCCTGTGCTTTTAGCTTGGCCTGCAGCACGGAC; CCTTCTCCCTCAATCCGGGCTGCCTCCGCACGGGACTCGGCCTCCGCCTTGGCAGTTCCG; GTGCTCTCCACGGCCATGCTCAGAGCCTCCAGCTCCAAAAGTTCCTTGCGAGCCTTTCTC; GGTTCTGACCGGGCCAGGATCCTCTGCCGCTCAAGCCGGCCCGGGGGCTCTCTGCTCCAG; TCTCCTGACCCCAATGCTTGGCCGCCGCCTCCTGGGAGTTGTCGGGGATCCCCATGGCCA; ACTTGAACCCTGGGTTCAAGGGCGTCCCGGGTCCTCTTGATACAAAGCTCCACTGACTGC; ACGTCCCACTGCTGGACCCAACCCGTTTGGTGGAATTACGCCTGTCCCAGGCTCGGGCAG; GGCCTTCCTCGGGGCCCTTCCTTCCCAGGCTAAAAGCACAACGAATGCAAGGAGCGGCCT; GGTCTCTTGGAAACACCCAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005115
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005115.3, NP_005106.2
• RefSeq Size: 2821 bp
• RefSeq ORF: 2682 bp
• Locus ID: 9961
• UniProt ID: Q14764
• Cytogenetics: 16p11.2
• Domains: Vault
• Protein Families: Druggable Genome
• Summary: This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (2, also known as S-MVP) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform 1.