LGI1 (NM_005097) Human Untagged Clone
$519.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | LGI1 |
| Synonyms | ADLTE; ADPAEF; ADPEAF; EPITEMPIN; EPT; ETL1; IB1099 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_005097 |
| Insert Size | 2240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005097.1, NP_005088.1 |
| RefSeq Size | 2254 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 9211 |
| UniProt ID | O95970, A0A0S2Z4S7 |
| Cytogenetics | 10q23.33 |
| Domains | EPTP, LRR, LRRCT, LRR_TYP |
| Protein Families | Druggable Genome, Secreted Protein |
| Summary | This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Reviews
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Resources
"NM_005097" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205340 | LGI1 (Myc-DDK-tagged)-Human leucine-rich, glioma inactivated 1 (LGI1) | 10 ug |
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| RC205340L1 | Lenti ORF clone of Human leucine-rich, glioma inactivated 1 (LGI1), Myc-DDK-tagged | 10 ug |
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| RC205340L2 | Lenti ORF clone of Human leucine-rich, glioma inactivated 1 (LGI1), mGFP tagged | 10 ug |
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| RC205340L3 | Lenti ORF clone of Human leucine-rich, glioma inactivated 1 (LGI1), Myc-DDK-tagged | 10 ug |
$819.00
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| RC205340L4 | Lenti ORF clone of Human leucine-rich, glioma inactivated 1 (LGI1), mGFP tagged | 10 ug |
$819.00
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| RG205340 | LGI1 (tGFP-tagged) - Human leucine-rich, glioma inactivated 1 (LGI1) | 10 ug |
$489.00
$719.00
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Citations
ADAM23 is a negative regulator of Kv1.1/Kv1.4 potassium currents
Lancaster, Burnor, Zhang et al
Neurosci. Lett. (2019) 704, 159-163 DOI: 10.1016/j.neulet.2019.04.012
Lancaster, Burnor, Zhang et al
Neurosci. Lett. (2019) 704, 159-163 DOI: 10.1016/j.neulet.2019.04.012
LGI1 antibodies alter Kv1.1 and AMPA receptors changing synaptic excitability, plasticity and memory
Petit-Pedrol, Sell, Planagumà et al
Brain (2018) 141 (11), 3144-3159 DOI: 10.1093/brain/awy253
Petit-Pedrol, Sell, Planagumà et al
Brain (2018) 141 (11), 3144-3159 DOI: 10.1093/brain/awy253
AI Summary: OriGene’s human LGI1 plasmid (human sequence sc-116925) was used to transfect HEK293 cells, enabling the study of antibody interactions with LGI1 in this research on synaptic excitability and memory.
Snippet: See Debanne and El Far (doi:10.1093/brain/awy271) for a scientific commentary on this article.Anti-LGI1 encephalitis is the most frequent cause of autoimmune li.;HEK293 cells seeded in P100 dishes at a density of 2 106 cells were grown to a confluence of 70% and transfected with a human LGI1 plasmid (Origene, human sequence sc-116925) using LipofectamineTM 2000 (Invitrogen) as reported (Lai et al., 2010)
Investigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series
Lai, Huijbers, Lancaster et al
Lancet Neurol (2010) 9 (8), 776-85 DOI: 10.1016/S1474-4422(10)70137-X
Lai, Huijbers, Lancaster et al
Lancet Neurol (2010) 9 (8), 776-85 DOI: 10.1016/S1474-4422(10)70137-X
AI Summary: In this study, OriGene’s human LGI1 cDNA clone (Cat# RC206307) and ADAM22 cDNA clone (Cat# RC208355) were used to transfect HEK293 cells, enabling the identification of LGI1 as the true autoantigen in limbic encephalitis previously attributed to potassium channels.
Showing 1-3 of 3 papers.
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