LGI1 (NM_005097) Human Untagged Clone

SKU SC116925

LGI1 (untagged)-Human leucine-rich, glioma inactivated 1 (LGI1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LGI1
• Synonyms: ADLTE; ADPAEF; ADPEAF; EPITEMPIN; EPT; ETL1; IB1099
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116925 sequence for NM_005097 edited (data generated by NextGen Sequencing); ATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGCCTGCATTCCCCTGAAAAGAATTGCT; TATTTCCTATGTCTCTTATCTGCGCTTTTGCTGACTGAGGGGAAGAAACCAGCGAAGCCA; AAATGCCCTGCCGTGTGTACTTGTACCAAAGATAATGCTTTATGTGAGAATGCCAGATCC; ATTCCACGCACCGTTCCTCCTGATGTTATCTCATTATCCTTTGTGAGATCTGGTTTTACT; GAAATCTCAGAAGGGAGTTTTTTATTCACGCCATCGCTGCAGCTCTTGTTATTCACATCG; AACTCCTTTGATGTGATCAGTGATGATGCTTTTATTGGTCTTCCACATCTAGAGTATTTA; TTCATAGAAAACAACAACATCAAGTCAATTTCAAGACATACTTTCCGGGGACTAAAGTCA; TTAATTCACTTGAGCCTTGCAAACAACAATCTCCAGACACTCCCAAAAGATATTTTCAAA; GGCCTGGATTCTTTAACAAATGTGGACCTGAGGGGTAATTCATTTAATTGTGACTGTAAA; CTGAAATGGCTAGTGGAATGGCTTGGCCACACCAATGCAACTGTTGAAGACATCTACTGC; GAAGGCCCCCCAGAATACAAGAAGCGCAAAATCAATAGTCTCTCCTCGAAGGATTTCGAT; TGCATCATTACAGAATTTGCAAAGTCTCAAGACCTGCCTTATCAATCATTGTCCATAGAC; ACTTTTTCTTATTTGAATGATGAGTATGTAGTCATCGCTCAGCCTTTTACTGGAAAATGC; ATTTTCCTTGAATGGGACCATGTGGAAAAGACCTTCCGGAATTATGACAACATTACAGGC; ACATCCACTGTAGTATGCAAGCCTATAGTCATTGAAACTCAGCTCTATGTTATTGTGGCC; CAGCTGTTTGGTGGCTCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAAA; ATCCAGGATATTGAAATTCTCAAAATCCGAAAACCCAATGACATTGAAACATTCAAGATT; GAAAACAACTGGTACTTTGTTGTTGCTGACAGTTCAAAAGCTGGTTTTACTACCATTTAC; AAATGGAACGGAAACGGATTCTACTCCCATCAATCCTTACACGCGTGGTACAGGGACACT; GATGTGGAATATCTAGAAATAGTCAGAACACCTCAGACACTCAGAACGCCTCATTTAATT; CTGTCTAGTAGTTCCCAGCGTCCTGTAATTTATCAGTGGAACAAAGCAACACAATTATTC; ACTAACCAAACTGACATTCCTAACATGGAGGATGTGTACGCAGTGAAGCACTTCTCAGTG; AAAGGGGACGTGTACATTTGCTTGACAAGATTCATTGGTGATTCCAAAGTCATGAAATGG; GGAGGCTCCTCGTTCCAGGATATTCAGAGGATGCCATCGCGAGGATCCATGGTGTTCCAG; CCTCTTCAAATAAATAATTACCAATATGCAATTCTTGGAAGTGATTACTCCTTTACTCAA; GTGTATAACTGGGATGCAGAGAAAGCCAAATTTGTGAAATTTCAGGAATTAAATGTTCAG; GCACCAAGATCATTCACACATGTGTCCATTAATAAGCGTAATTTTCTTTTTGCTTCCAGT; TTTAAGGGAAATACACAGATTTACAAACATGTCATAGTTGACTTAAGCGCATGA; ; Clone variation with respect to NM_005097.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005097 unedited; NGGTTCANGATATTTGTATACGACTCACTATAGNNGGCGGCCGCGCAATTCGGCACGAGG; GAGCAGTGCATTGCTGGAGCGAGGAGAAGCTCACGAATCAGCTGCAGGTCTCTGTTTTGA; AAAAGCAGAGATACAGAGGCAGAGGAAAAGGGTGGACTCCTATGTGACCTGTTCTTAGAG; CAAGACAATCACCATCTGAATTCCAGAAGCCCTGTTCATGGTTGGGGATATTTTCTCGAC; TGCATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGCCTGCATTCCCCTGAAAAGAATT; GCTTATTTCCTATGTCTCTTATCTGCGCTTTTGCTGACTGAGGGGAAGAAACCAGCGAAG; CCAAAATGCCCTGCCGTGTGTACTTGTACCAAAGATAATGCTTTATGTGAGAATGCCAGA; TCCATTCCACGCACCGTTCCTCCTGATGTTATCTCATTATCCTTTGTGAGATCTGGTTTT; ACTGAAATCTCAGAAGGGAGTTTTTTATTCACGCCATCGCTGCAGCTCTTGTTATTCACA; TCGAACTCCTTTGATGTGATCAGTGATGATGCTTTTATTGGTCTTCCACATCTAGAGTAT; TTATTCATAGAAAACAACAACATCAAGTCAATTTCAAGACATACTTTCCGGGGACTAAAG; TCATTAATTCACTTGAGCCTTGCAAACAACAATCTCCAGACACTCCCAAAAGAATTTTCA; AAGGCCTGGATTCTTTAACANATGTGGACCTGAGGGGTAATTCATTTATTGTGACTGGTA; ACTGAAATGGCTAGTGGAATGGCTTGGCCACACCAATGCACTGTTGAAGACATCTACTGC; GAAGGCCCCCCAGATTACAGAGCGCAAATCATAGTCTCTCCTCGAAGATTTCGATTGCTC; ATTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005097 unedited; CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAAAAAATGTAAACAT; TTTATTTGGCAGTTGTTTCTGTACACATGAATAAATAAATGGAAAAGTACATATTAATAT; TTCTTTTTGGAGTAACAGATACCATTAAACGCTTATTTACACATCTTCACTGCAAAGAAC; AGTTACACAATTAAATTTTGTCTTATAAAAGGTAACTTCCCACATCACTGGACTGGACAG; TTAAAGGATGAATAACTGGTAAGGCTTGGTTTTAACAACCAAAAGTGTGGAAGGGGGTTT; TTTTTTGCGGGGGGGAAAAAAAAAAAAAACCCCCTTTTTGTTGTTTTTCCCGGGGGGTTT; TTGGGAAAAAAAAAATTTGTGGGGGGCGCCCACAAAAGAGGGGGGGGGGGCCCCCCCCCC; CAAAAAAAAAAAAAAGGGGTGGGGGGGNGGNTTTTTTTTTTTTTTTTTCTTTAAAAAGGG; GGGAGAAAAAAAAAAAANNCCTTTTTTTTTTTTTTCTCCCGCGGGGGGGGGGGGGGGTGT; CTTCCCCCCCCCCCCCCCTCTCTTATAATACAAAAACNNNTTTGTTTGGTTTTTTTTTTT; TCCCCCCCCGCCCCCGCGGGGGCGGGGGGGGGGGAGGGAGAAAAACACCCATCTCATTNN; TTTCTTTTTTTTTTTTTTTTTTTAGGAAAATAAGGGGGNGGGGGGGNNGCCCCCCCCNCT; CTCTCTTCNTTCTTTCCTCTCTCCTGTATCTCATACCCCTNCCCAACTCCCNCCATTANN; NNTATATGTTGNGGGAATGCAAAAGGGACTGCCGGGNGCTTCCTGCCCTCCGTTCGTNCC; TCCTCTCCTCCCATGCCCTTTTCACCGTAGTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005097
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005097.1, NP_005088.1
• RefSeq Size: 2254 bp
• RefSeq ORF: 1674 bp
• Locus ID: 9211
• UniProt ID: O95970
• Cytogenetics: 10q23.33
• Domains: EPTP, LRR, LRRCT, LRR_TYP
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).