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GCHFR (NM_005258) Human Untagged Clone

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SKU
SC116878
GCHFR (untagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR)
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GCHFR
Synonyms GFRP; HsT16933; P35
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_005258 edited
ATGCCCTACCTGCTCATCAGCACCCAGATCCGCATGGAGGTGGGCCCCACTATGGTGGGC
GATGAACAGTCGGATCCAGAGCTGATGCAGCATCTGGGGGCTTCAAAGAGAAGAGCCTTG
GGAAACAACTTTTATGAATACTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTG
GAACGCAGGGGCTTCCGTGTGCTGAGCATGACGGGGGTGGGCCAGACGCTGGTGTGGTGT
CTGCACAAGGAGTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005258 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATT
CGGCACGAGGGGGACTCCCAGCTGCGCGTCGCAGTCCCGACGCGAGAAGGGCTGGAGTCG
GCGTCCAGCCTAGAGCCCCCGGTGGGAGCCAGGCCGGGACGCGTGCACCATGCCCTACCT
GCTCATCAGCACCCAGATCCGCATGGAGGTGGGCCCCACTATGGTGGGCGATGAACAGTC
GGATCCAGAGCTGATGCAGCATCTGGGGGCTTCAAAGAGAAGAGCCTTGGGAAACAACTT
TTATGAATACTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTGGAACGCAGGGG
CTTCCGTGTGCTGAGCATGACGGGGGTGGGCCAGACGCTGGTGTGGTGTCTGCACAAGGA
GTGACCTTCTCATGCTGATTTGCAGACGGGGCACCCCTGTGGAGGGGCTGCTGTGGGCCC
TGACCTCCAAGCTCCTGCCTCACCGTCTGCCTTGCTCCTCTCTTCCCAAATCATCACCGC
CATGGGCCCAGCCCCAAAGGGCAGTGAATGGCCTTCTCTGAAACCCTGCGTCAAGCAGTG
GGAGAGGGCAGTGCCCGGTGCCCTGGTGCTCCCAGCTGCCCTCCTGCTTCGGGCCTGGGC
CGNAGGGCCTGTGTANGCCATGTTCCTNCGGCAGCTGCCCCGGGCCNNGAGCTGGCACTN
CAGCGGCCCCTGGCGCGTGGCTCCTGCATAGCTAGCCCAAGCAATAAAGGGCTGTGATGA
GTGGCTGCGCCTGTGCTCTGCTTGTGCACCGGACTCTGGACTCTCACCCTGCGGAGCGTC
CCTGGGGGGCTGGCCCTTNCGTGGAGGGCGCCCAGCCTGGNTGGACGCTCTGCGCAAACC
CAGCCCTATACTATGNTGGGGGTAAGGAGGAAAATCTAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005258 unedited
TGACCCCTTACCCTACATAGGTATAAGGTGCTGGGCTCTGCCGCAGAGCGCTCCACCCAG
GCCTGGCGCCCCTCCCAGGAGTGGGCAGACCCCCAGGGAAACGCTCCGCAGGGTGAGAGT
CCCAGAGTCCGTGCACAAGCAGAGCACAGGCGCAGCCACTCATCACAGCCCTTTATTGGC
TTGGGCTAGCTATGCAGGAGCCACGCGCCAGGGCCGCTGGAGTGCCCAGCTCCGGCCCGG
GGCAGCTGCCCGAGGAACATGGCCTACACAAGGCCCTCGGCCCAGGCCCGAAGCAGGAGG
GCAGCTGGGAGCACCAGGGCACCGGGCACTGCCCTCTCCCACTGCTTGACGCAGGGTTTC
AGAGAAGGCCATTCACTGCCCTTTGGGGCTGGGCCCATGGCGGTGATGATTTGGGAAGAG
AGGAGCAAGGCAGACGGTGAGGCAGGAGCTTGGAGGTCAGGGCCCACAGCAGCCCCTCCA
CAGGGGTGCCCCGTCTGCAAATCAGCATGAGAAGGTCACTCCTTGTGCAGACACCACACC
AGCGTCTGGCCCACCCCCGTCATGCTCAGCACACGGAAGCCCCTGCGTTCCAGCTTGTCC
AGGACTATGCGGGGAGGGTCATCGACGTAGTATTCATAAAAGTTGTTTCCCAAGGCTCTT
CTCTTTGAAGCCCCCAGATGCTGCATCAGCTCTGGATCCGACTGTTCATCGCCCACCATA
GTGGGGCCCACCTNCATGCGGATCTGGGTGCTGATGAGCAGGTAGGGCATGGTGCACCGC
GTCCCGGCCTGGCTCCCACCGGGGGCTCTAGCTGGACGCCGACTCCAACCCTTNTCGCGT
TGGGACTGCGAACCGCAACTGGGAGTTCCCCTCGTGCCGAATTCCGGACGAAGCCCTCTT
GCCGAATTCCCGGCCGNCCTTATATGAGTGGTATAACAAATCTGGACGTTACTAAAGAGC
CTGCTTATTAGACTTCN
Restriction Sites NotI-NotI |
ACCN NM_005258
Insert Size 1090 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005258.2, NP_005249.1
RefSeq Size 713 bp
RefSeq ORF 255 bp
Locus ID 2644
UniProt ID P30047
Cytogenetics 15q15.1
Summary GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. provided by RefSeq, Jul 2008
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"NM_005258" in other vectors (4)
SKU Description Size Price
RC223674 GCHFR (Myc-DDK-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) 10 ug
$150.00
RC223674L3 Lenti-ORF clone of GCHFR (Myc-DDK-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) 10 ug
$450.00
RC223674L4 Lenti-ORF clone of GCHFR (mGFP-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) 10 ug
$450.00
RG223674 GCHFR (tGFP-tagged) - Human GTP cyclohydrolase I feedback regulator (GCHFR) 10 ug
$350.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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