PODXL (NM_005397) Human Untagged Clone

SKU SC116789

PODXL (untagged)-Human podocalyxin-like (PODXL), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PODXL
• Synonyms: gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005397, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC; CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC; GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG; GCCAACGAAATCTTGGCCTCGGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA; CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC; TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA; GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC; ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT; TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG; AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA; CCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGC; CCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAG; ACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGG; CTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCT; GAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGA; GCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTC; AGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAA; GGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAG; GAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCG; TGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGA; GCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATG; CAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGA; CCAAGGACGACCTGGATGAGGAGGAAGACACACACCTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005397 unedited; NTTTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA; TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG; CCGCGAATTCGCACGAGGCGCCGCAGCGCGACTCGGGAGCCCCGGGCCACAGCCTGGCCT; CCGGAGCCACCCACAGGCCTCCCCGGGCGGCGCCCACGCTCCTACCGCCCGGACGCGCGG; ATCCTCCGCCGGCACCGCAGCCACCTGCTCCCGGCCCAGAGGCGACGACACGATGCGCTG; CGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTC; GCCGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAA; CAAAACAGCACCGACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCA; GAGCACAGTCCCCACTTCCAAGGCCAACGAAATCTTGGCCTCAGTCAAGGCGACCACCCT; TGGTGTATCCAGTGACTCACCGGGGACTACAACCCTGGCTCAGCAAGTCTCAGGCCCAGT; CAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTACTACCACCATCGAGAGCCC; CAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACAGCTAAACC; TAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAG; CCACAGTGTGACCACAGACCTCACATCCACTAAGGGCAGACATCTGACGACCCCTCACCC; CTACAGTCCACTTAGCCCCCGACACCCCACTTGACGCATNCTGTGGCCACCCCCACAGCT; CGGGACATGACCATCTTATGAAANTTNCAAGCAGTTCAGCACTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005397 unedited; NCGGTTTCGCTATGGNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTA; CACAAAAACACTTTAATTGACAGTATACAATTTTCCAAAATATATTTTTGTAAGAAAATG; CAATAATTATTAACTATAGTTTTTACAAACAAGTTTCTCAGTAAATTCCAGTGTACTTCA; GACCCCTGTCCACTAAGACATATATGATCCCCCAGTTCCTGGGCAAACTGTTGAACATTC; ACTGCAGACAAAAAGACCAACACCAAAGAGTCATCTGTGTCCTCCATGCTGTGTTTGCAG; CAGACCTGAGGGATCAGCTAGTGACCGTGACAAAAGCTATGCTACAGTTTTACTCTTGCC; CTCTCTGCCTCCCCCATCATGTTTCCTTGGTCCCTCAGTCCTGTTGGAGTAAGTTCCATA; TATAAAAGCTCATCCAAAACTTTGTTTCTTGGCAGAAAGAAAGAATTGTTCACTTACATC; TTAAACCTAAATGATTCAGAGAAAGTGTAGAAGACCAGTTCTTATGTTTGTGTTCATGCC; CTCCCTAGCCTTGCTAGGGTAACAACTGGGAAAGGAATTCTGTCCTTTGTGTCATTCTGG; CACCTTCGAGTAGCAATTCTTAATTTACCGATTATTCCTTCTGAGGCGCACAGTCTGTGG; ATCCAGGGTTTAAACTTGGTTCTGTCCTGAACCATGTGATTTTGAGGAAGTCACTTCATC; TTTGTGAGCTTCAAATTATCTTTTTCTCTTCAAAAAGAATGGACTAGATCTTGGAAAACC; TTTCTCCATGACTACAGGGGGTGGCTTGCCACCCAAGCATATTGGCTCCAACCTTTACTG; GAAATGGAGCTGGGACCTTTCTGGGGAGGGGGGCAGGGGTTGGCTTTTAAAATTAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_005397
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005397.2, NP_005388.2
• RefSeq Size: 5903 bp
• RefSeq ORF: 1581 bp
• Locus ID: 5420
• UniProt ID: O00592
• Cytogenetics: 7q32.3
• Protein Families: Transmembrane
• Summary: This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an in-frame alternate exon compared to variant 1, resulting in a shorter protein (isoform 2).