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TAF11 (NM_005643) Human Untagged Clone

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SKU
SC116607
TAF11 (untagged)-Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TAF11
Synonyms MGC:15243; PRO2134; TAF2I; TAFII28
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_005643, the custom clone sequence may differ by one or more nucleotides


ATGGACGATGCCCACGAGTCGCCCTCCGACAAAGGTGGAGAGACAGGGGAGTCGGATGAGACGGCCGCTG
TGCCCGGGGACCCGGGGGCTACCGACACCGATGGAATCCCAGAGGAAACTGACGGAGACGCAGATGTGGA
CTTGAAAGAAGCTGCAGCGGAGGAAGGCGAGCTCGAGAGTCAGGATGTCTCAGATTTAACAACAGTTGAA
AGGGAAGACTCATCATTACTTAATCCTGCAGCCAAAAAACTGAAAATAGATACCAAAGAAAAGAAAGAGA
AAAAGCAGAAAGTAGATGAAGATGAGATTCAGAAGATGCAAATCCTGGTTTCTTCTTTTTCTGAGGAGCA
GCTGAACCGTTATGAAATGTATCGCCGCTCAGCTTTCCCTAAGGCAGCCATCAAAAGGCTGATCCAGTCC
ATCACTGGCACCTCTGTGTCTCAGAATGTTGTTATTGCTATGTCTGGTATTTCCAAGGTTTTCGTCGGGG
AGGTGGTAGAAGAAGCACTGGATGTGTGTGAGAAGTGGGGAGAAATGCCACCACTACAACCCAAACATAT
GAGGGAAGCCGTTAGAAGGTTAAAGTCAAAAGGACAGATCCCTAACTCGAAGCACAAAAAAATCATCTTC
TTCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_005643 unedited
TCAGCATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCCTCA
GGCATCTCCGCGATCTCCTCTCCCCTCCAATCCTATCCGTGATGGACGATGCCCACGAGT
CGCCCTCCGACAAAGGTGGAGAGACAGGGGAGTCGGATGAGACGGCCGCTGTGCCCGGGG
ACCCGGGGGCTACCGACACCGATGGAATCCCAGAGGAAACTGACGGAGACGCAGATGTGG
ACTTGAAAGAAGCTGCAGCGGAGGAAGGCGAGCTCGAGAGTCAGGATGTCTCAGATTTAA
CAACAGTTGAAAGGGAAGACTCATCATTACTTAATCCTGCAGCCAAAAAACTGAAAATAG
ATACCAAAGAAAAGAAAGAGAAAAAGCAGAAAGTAGATGAAGATGAGATTCAGAAGATGC
AAATCCTGGTTTCTTCTTTTTCTGAGGAGCAGCTGAACCGTTATGAAATGTATCGCCGCT
CAGCTTTCCCTAAGGCAGCCATCAAAAGGCTGATCCAGTCCATCACTGGCACCTCTGTGT
CTCAGAATGTTGTTATTGCTATGTCTGGTATTTCCAAGGTTTTCGTCGGNGAGGTGGTAG
AAGAAGCACTGGATGTGTGTGAGAAGTGGGGAGAAATGCCACCACTACAACCCCAAACAT
ATGAGGGAAGCCGTTAGAAGGTTAAAGTCAAAAGGACAGATCCCTAACTCGAAGCACAAA
AAAATCATCTTTCTTCTAGACCAAAGTCTAGAAAGGCCCTATGTACTGACGGAAGAAGTA
TTGGTTCCAGACTTCCTATTAGACTGGCTGCATTGGTGCTTTAATATCTCCAGCCTCCCA
GGATTCCATGATGATTNTAANGGCTTTCTCAAAACTCTGAATTTGTCCCACCTAAAAGGA
TGGACCCTGATGAAACTGGCCTGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005643 unedited
GCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGTTGGAAAACAGTTTA
ATGATCACTCACCAAAATCCACAGGAAGAATCTTAAATGTTTACAAGCACCAATTATTCT
GCTATTCCTGCCATCACCGCATCCTTCATGGTAGAGTATCACAAGTAAAAGTTTCTGGTT
GTTTCATCTACTTAAAACCAGATATAAGAAACAACCTAAGTCTTAGCAACTTCAGGCTTC
AATGTGAAACCATTAAAGCCCTCAGCACTTTAGGAGGCTGAGGCAGGAGGACTGCTTGAA
GCCAGGAGTTCACGACCAGCCTGGGCAACAAAGCAAGACCCCATCTCCATAAAAAATAAA
AATAAGTTAGCTGGGCACAGTAGTGTGTGCCTGTAGTCCTAGCTACTCAGGAGACTGAGG
TGGGAGGGTCACTTAAGCCCAGGAGTTCAAGGCTGCAGTCATGCCGCTGCACTCCAGCCT
AGGTGATAGAGCAAGACCCTATCTCANACAAAACGAAACCAAAACAAAACAAAACTCTCT
TGGAAAGGTTTTCTCTCTGAAAGCAGTTCCCCTGTCCAGAAGATGCTTATGGCCCACGTA
TCTTCTTTCAACTGGTCAAGACAGTTAAATACTTCAAAATGCCCCCAAGAGTCCCAAAAT
TTAGTCAAGGCAAGTATCAATCAGGCTACATACTTTCTAGGTGTGACAAATATCAGAGTT
TTGAGAAAGACTTTAAAATCATCCTGGAATTCTTGGNAGCCTGAGATACTTAAGCCACCA
TGCAGACAGTTTTATAAGAAGTTCTGAACCAATACTCTTCCGTCAGTAACATAGGCCTTC
TAGACCTTGGTCTAAAGAAGAGATTTTTTTGGCCTCAGTTAGGGAACTGCCCTTTGACTT
TAACCTCTAAACGGTCCTTAATAGTTGGGTTGGAAGGGC
Restriction Sites NotI-NotI |
ACCN NM_005643
Insert Size 1710 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005643.2, NP_005634.1
RefSeq Size 1599 bp
RefSeq ORF 636 bp
Locus ID 6882
UniProt ID Q15544
Cytogenetics 6p21.31
Domains TAFII28
Protein Families Transcription Factors
Protein Pathways Basal transcription factors
Summary Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2012
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_005643" in other vectors (4)
SKU Description Size Price
RC203466 TAF11 (Myc-DDK-tagged)-Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11) 10 ug
$300.00
RC203466L3 Lenti ORF clone of Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11), Myc-DDK-tagged 10 ug
$600.00
RC203466L4 Lenti ORF clone of Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11), mGFP tagged 10 ug
$600.00
RG203466 TAF11 (tGFP-tagged) - Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11) 10 ug
$489.00 $500.00

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