TAF7 (NM_005642) Human Untagged Clone

SKU SC116606

TAF7 (untagged)-Human TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa (TAF7)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TAF7
• Synonyms: TAF2F; TAFII55
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005642, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGTAAAAGCAAAGATGATGCTCCTCACGAACTGGAGAGCCAGTTTATCTTACGTCTGCCTCCAGAAT; ATGCCTCTACTGTGAGAAGGGCAGTACAGTCTGGTCATGTCAACCTCAAGGACAGACTGACAATTGAGTT; ACATCCTGATGGGCGTCATGGAATCGTCAGAGTGGACCGTGTTCCATTGGCCTCAAAATTAGTAGACCTG; CCCTGTGTTATGGAAAGCTTGAAAACCATTGATAAAAAAACTTTTTACAAGACAGCTGATATCTGTCAGA; TGCTTGTATCCACAGTTGATGGTGATCTCTATCCTCCTGTGGAGGAGCCAGTTGCTAGCACTGATCCTAA; AGCAAGCAAGAAAAAGGATAAGGACAAAGAGAAAAAGTTTATCTGGAACCACGGAATTACTCTGCCTCTA; AAGAATGTCAGGAAGAGAAGGTTCCGGAAGACAGCAAAGAAGAAATATATTGAATCTCCAGATGTTGAAA; AAGAAGTGAAACGATTGCTGAGTACAGATGCTGAAGCTGTTAGTACTCGGTGGGAAATAATTGCCGAAGA; TGAAACAAAGGAGGCAGAAAATCAAGGCCTGGATATCTCTTCTCCAGGAATGTCTGGTCACAGGCAGGGC; CATGACTCATTAGAACATGATGAGCTTCGGGAGATATTCAATGACCTCAGCAGCAGCAGTGAGGATGAAG; ATGAGACCCAGCATCAAGATGAAGAAGATATAAACATCATTGACACGGAGGAAGATCTGGAGAGACAGCT; ACAGGACAAGCTAAATGAATCAGATGAACAGCACCAGGAAAATGAAGGAACCAATCAGCTGGTTATGGGA; ATTCAGAAGCAGATTGACAACATGAAAGGCAAGCTCCAAGAGACCCAGGACAGGGCAAAACGACAAGAGG; ATCTCATCATGAAAGTGGAAAATCTGGCTCTCAAGAACAGATTTCAGGCTGTACTGGATGAGCTCAAACA; AAAGGAAGACCGAGAAAAGGAGCAACTCAGCTCTTTGCAAGAGGAGCTAGAATCACTCCTAGAGAAGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005642 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGG; CACGAGGGCGACCCGGACGGAGGGCCGGCGAGGTGCGGGGTCTGGTGATGCGAGCTGCGC; CTCTCGGCAAGATTTCGCGCTGCCCATCCCGGGCCCTTTCATCAGTAATCGGTAGTGGAT; CACTCTGCCAAGCGGCAGGAAGAATTAAGGAAACGACAAGGAGACGCTCGGCTCTCTCCC; GCTTGGCTCCTTGCGGCCTCCTCTTCCCTTCGCTCCGGCCCGGTGAAACTGAACTTATAA; TCGTCACTGGATTGTAAGTACCCGAGGCGAAGAGAGCTCGCTGAGCCCTGATTTTTTGAG; TGTCTTTGTTCCGGGAGAGTTTGTGAGTTGAAAGTATCTCTGCTGGGCTTTCTGGGCCGA; AAACCGTTCCGGGGGAGCCGCCATTTGCTTTCCTGTTCCCTAGCTAGCTAGCTAGCTCTC; TCCGCGTTGTCCGGCAGCGGCACCTAGAGGTTGGGACTTGGCATTGCATCTGATTTAATG; AACTTAAGTCTGTGAATAAGCCTTTGTGTTAACGACTGGTATTCGGTCACAGCATATTTA; GAGAAAAGACTTGGAGCTTAAATAAAAACTAAGGCANAATAGACGCTTAGCTGCTGATCT; ACAGAGAAACTTCTGTATTAAAAGATTTCATTCATAGCANACTGGGTGTTTAAACTATNG; CAGTAGCTGGAACTTTNNTAGTGTAACCAGCATTTATGGNGAGAAAGTGATCACANGGNA; AATAAGATGAGTNAAAGCAAAGATGATGCCTCCTNACGAACTGGAGAAGCCAGTTATCTT; ACGTCTGCCTCCANAATTGCCTTTACTGTGAGAAAGGCATACAGTCTGGTCATGTCAACC; TCAGGACGACTGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005642 unedited; TGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAAACAGAGCT; TTTATTTATTGTATTGGAAACAACTTAATAATTTGCATCTCTACATATAGAAAGCTGCTT; TGAATAACTGGGAAAACAACTATTGCATAGGAAAACATATGCAAACTAGCATCATTGTCT; CTAGACTAGGTTCTTCTTATTGATCTCACGTCATCACAAAGCAGCAACAGCAGCTTCTAG; AAAATGGAGAAACAGTAAAGTCCTGCAATGAAGCTATAATTAAGGTTGAACCATACATTC; CGCTAATCCTGCAACTTTCCTACAATCATTTTTCTGCCTATACAATCCTGCTTCTTATAG; GATGAACACCTAGCAAAACAATATAAATTTGCTGAAAAACAAAATTACAAACAAATGAAA; GAACTTAACAGAACACACAGCATTAAAAGGACTAGGAAGAGCAAGGTCTTAATACCCAGT; ACAATAAAGCCAAGAATTTTCTAATGCTGACCAGTCTGAAGACTGAAATTAAATATCAGT; TCTTTTTACTTCTCTAGGAGTGATTCTAGCTCCTCTTGCAAAGAGCTGAGTTGCTCCTTT; TCTCGGTCTTCCTTTTGTTTGAGCTCATCCAGTACAGCCTGAAATCTGTTCTTGAGAGCC; AGATTTTCCACTTTCATGATGAGATCCTCTTGTCGTTTTGCCCTGTCCTGGGTCTCTTGG; AGCTTGCCTTTCATGTTGTCAATCTGCTTCTGAATTCCCATACCAGCTGATGGTTCCTTC; ATTTCCTGGNGCTGNTCATCTGATTCATTTAGCTTGCCTGTAGCTGTCTCTCCAGATCTT; CCTNCGTGTCATGGAATGTTATATCTCTCAATCTGATGCTGGNCTCANTTCATCCTACTG; CTGTGCTNGAGTCATTGATATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005642
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005642.2, NP_005633.2
• RefSeq Size: 2310 bp
• RefSeq ORF: 1050 bp
• Locus ID: 6879
• UniProt ID: Q15545
• Cytogenetics: 5q31.3
• Domains: TAFII55_N
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Summary: The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II. [provided by RefSeq, Jul 2008]