CALCOCO2 (NM_005831) Human Untagged Clone

SKU SC116483

CALCOCO2 (untagged)-Human calcium binding and coiled-coil domain 2 (CALCOCO2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CALCOCO2
• Synonyms: NDP52
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116483 sequence for NM_005831 edited (data generated by NextGen Sequencing); ATGGAGGAGACCATCAAAGATCCCCCCACATCAGCTGTCTTGCTGGATCACTGTCATTTC; TCTCAGGTCATCTTTAACAGTGTGGAGAAGTTCTACATCCCTGGAGGGGACGTCACATGT; CATTATACCTTCACCCAGCATTTCATCCCTCGTCGAAAGGATTGGATTGGCATCTTTAGA; GTGGGGTGGAAGACAACCCGTGAGTATTACACCTTCATGTGGGTTACTTTGCCCATTGAC; CTAAACAACAAATCAGCTAAACAGCAGGAAGTCCAATTCAAAGCTTACTACCTGCCCAAG; GATGATGAGTATTACCAGTTCTGCTATGTGGATGAGGATGGTGTGGTCCGGGGAGCAAGT; ATTCCTTTCCAATTCCGTCCAGAAAATGAGGAAGACATCCTGGTTGTTACCACTCAGGGA; GAGGTGGAAGAGATTGAGCAGCACAACAAGGAGCTTTGCAAAGAAAACCAGGAGCTGAAG; GACAGCTGTATCAGCCTCCAGAAGCAGAACTCAGACATGCAGGCTGAGCTCCAAAAGAAG; CAGGAGGAGCTAGAAACCCTACAGAGCATCAATAAGAAGTTGGAACTGAAAGTGAAAGAA; CAGAAGGACTATTGGGAGACAGAGCTGCTTCAACTGAAAGAACAAAACCAGAAGATGTCC; TCAGAAAATGAGAAGATGGGAATCAGAGTGGATCAGCTTCAGGCCCAGCTGTCAACTCAA; GAGAAAGAAATGGAGAAGCTTGTTCAGGGAGATCAAGATAAGACAGAGCAGTTAGAGCAG; CTGAAAAAGGAAAATGACCACCTCTTTCTCAGTTTAACTGAACAGAGGAAGGACCAGAAG; AAGCTCGAGCAGACAGTGGAGCAAATGAAGCAGAATGAAACTACTGCAATGAAGAAACAA; CAGGAATTAATGGATGAAAACTTTGACCTGTCAAAAAGACTGAGTGAGAACGAAATTATA; TGTAATGCTCTGCAGAGACAGAAAGAGAGATTGGAAGGAGAAAATGATCTTTTGAAGAGG; GAGAACAGCAGATTGCTCAGTTACATGGGTCTGGATTTTAATTCTTTGCCGTATCAAGTA; CCTACTTCAGATGAAGGAGGCGCAAGACAAAATCCAGGACTTGCCTATGGAAACCCATAT; TCTGGTATCCAAGAAAGTTCTTCCCCCAGCCCGCTCTCCATCAAGAAATGCCCTATCTGC; AAAGCAGATGATATTTGTGATCACACCTTGGAGCAACAGCAGATGCAGCCCCTTTGTTTC; AATTGTCCAATTTGTGACAAGATCTTCCCAGCTACAGAGAAGCAGATCTTTGAAGACCAC; GTGTTCTGCCACTCTCTCTGA; ; Clone variation with respect to NM_005831.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005831 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGCTGTCGCGCCGCTGCTGGT; TGCTGTCCCTGGACCCCTACCATGGAGAGACCATCAAAGATCCCCCCACATCAGCTGTCT; TGCTGGATCACTGTCATTTCTCTCAGGTCATCTTTAACAGTGTGGAGAAGTTCTACATCC; CTGGAGGGGACGTCACATGTCATTATACCTTCACCCAGCATTTCATCCCTCGTCGAAAGG; ATTGGATTGGCATCTTTAGAGTGGGGTGGAAGACAACCCGTGAGTATTACACCTTCATGT; GGGTTACTTTGCCCATTGACCTAAACAACAAATCAGCTAAACAGCAGGAAGTCCAATTCA; AAGCTTACTACCTGCCCAAGGATGATGAGTATTACCAGTTCTGCTATGTGGATGAGGATG; GTGTGGTCCGGGGAGCAAGTATTCCTTTCCAATTCCGTCCAGAAAATGAGGAAGACATCC; TGGTTGTTACCACTCAGGGAGAGGTGGAAGAGATTGAGCAGCACAACAAGGAGCTTTGCA; AAGAAAACCAGGAGCTGAAGGACAGCTGTATCAGCCTCCAGAAGCAGAACTCAGACATGC; AGGCTGAGCTCCAAAAGAAGCAGGAGGAGCTAGAAACCCTACAGAGCATCAATAAGAAGT; TGGAACTGAAAGTGAAAGAACAGAAGGACTATTGNGAGACAGAGCTGCTTCAACTGAAAG; AACANAACCAGAAGATGTCCTCAGAAATGAGAAGATGGGAATCAGAGTGGATCAGCTTCA; NGCCCAGCTGTCAACTCAAGAGAAAGAAATGGAGAAAGCTGTTCAGGGNAGATCAAGATA; GACAGAGCAGTTNGAGCAGCTGAAAAAGGAAATGACCACCTCTTTCTCAGTTNNACTGAC; AGAGGAAGGACCANNAGAAGCTCGGCCGACAGTGGACCAATGAAGCAGATGGACTACTGC; ATGAAGAACAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005831 unedited; ATTACCTTAGTNCCCTGNAATAGNAGAATAATTTATTTTTAGTATTAAAGAGATTAACAA; GTTGTCTCATTATTCTGATTTACCCATATTCAAAACTCACCTACTCAGAAAACTGCAAAC; CCATAGTTGCTAACAATCCTATTTAACCATTAAGAAAGGATTTACAACAATAAAAGCTAT; GTACATAGGAAATATCAGAGAGTAGAAAGTGCTAACCAGAGGTACCCAGAAAAGCTTTCT; GTGACTGAAACAATACTTCCTGTCTAAGGTTCAGGAGTAAGATGCTTCTTTGGGGGCTGA; GAAATTAACATGAGAAACCTTTGGTATCCAAATTGGATTTCTGAGCTCTTTGCCACGGCA; TTTTTGGCTAGTCTAAAACAATCATTTGGAGAAAGCAACACCAAGGATGAGGAGGAAGGA; ATTCTCAACCTCGGACCAATCTCTGGCCCCAGGGTTAGGACTGAGAACAAATACACTCAA; GGGCTGACTTTGAATGGCAGTGGGACAACTTGGAAAACTAATCACTTAGTATCAAGTCAG; GCCTGTTGTGAATACCCAGGTTGGCTCCATCCCAGGGCCCTTGTACTGAGACCCTCAGAA; GAATGACAGGTGAGAGGATGATACAGCTAGAGTTATGCGACTTAAAGGTAGCAACCCACA; AGGATGGCAGCAGAAGCCCTTACAGTGACCTTCAGATCAGTGACATAACTNCACTGTTAG; CGGCAGCAGGGCTGAGTAAATNCCTAATAATTTCAGGTTAGGCAGGATCTTGACTCATAT; AACTCATGGTAGAAGCTATAGGNTCTATTCTATAAAATCTCTGTATACATCCAAGAAGGT; GGGATACTCAGAGAGAGTGGCAGAACACGTGGGTCTTCAAAGATCTGCTCTCTGTAGCTG; GGAAGATCTTGNCACAAATTGGACAATTGAACAAAGGGGCTGCATCTGCTGGTGCTCCAA; GGGTGATCACAAAATCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005831
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005831.3, NP_005822.1
• RefSeq Size: 3299 bp
• RefSeq ORF: 1341 bp
• Locus ID: 10241
• UniProt ID: Q13137
• Cytogenetics: 17q21.32
• Summary: This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (3) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.