DNAJA2 (NM_005880) Human Untagged Clone

SKU SC116463

DNAJA2 (untagged)-Human DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNAJA2
• Synonyms: CPR3; DJ3; DJA2; DNAJ; DNJ3; HIRIP4; PRO3015; RDJ2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116463 sequence for NM_005880 edited (data generated by NextGen Sequencing); ATGGCTAACGTGGCTGACACGAAGCTGTACGACATCCTGGGCGTCCCGCCCGGCGCCAGC; GAGAACGAGCTGAAGAAGGCATACAGAAAGTTAGCCAAGGAATATCATCCTGATAAGAAT; CCAAATGCAGGAGACAAATTTAAAGAAATAAGTTTTGCATATGAAGTACTATCAAATCCT; GAGAAGCGTGAGTTATATGACAGATACGGAGAGCAAGGTCTTCGGGAAGGCAGCGGCGGA; GGTGGTGGCATGGATGATATTTTCTCTCACATTTTTGGTGGGGGATTGTTCGGCTTCATG; GGCAATCAGAGTAGAAGTCGAAATGGCAGAAGAAGAGGAGAGGACATGATGCATCCACTC; AAAGTATCTTTAGAAGATCTGTATAATGGCAAGACAACCAAACTACAACTTAGCAAGAAT; GTGCTCTGTAGTGCATGCAGTGGCCAAGGCGGAAAGTCTGGAGCTGTCCAAAAGTGTAGT; GCTTGTCGAGGTCGAGGTGTGCGCATCATGATCAGACAGCTGGCTCCAGGGATGGTACAA; CAGATGCAGTCTGTGTGCTCTGATTGTAATGGAGAAGGAGAGGTAATTAATGAAAAAGAC; CGCTGTAAAAAATGTGAAGGGAAGAAGGTGATTAAAGAAGTCAAGATTCTTGAAGTCCAC; GTAGACAAAGGCATGAAACATGGACAGAGAATTACATTCACTGGGGAAGCAGACCAGGCC; CCAGGAGTGGAACCCGGAGACATTGTTCTTTTGCTACAGGAGAAAGAACATGAGGTATTT; CAGAGAGATGGGAATGATTTGCACATGACATATAAAATAGGACTTGTTGAAGCTCTATGT; GGATTTCAGTTCACATTTAAGCACCTTGATGGACGTCAGATTGTGGTGAAATACCCCCCT; GGCAAAGTAATTGAACCAGGGTGTGTTCGTGTAGTTCGAGGTGAAGGGATGCCGCAGTAT; CGTAATCCCTTTGAAAAAGGTGATCTTTACATAAAGTTTGATGTGCAGTTTCCTGAAAAC; AACTGGATCAACCCAGACAAGCTTTCTGAACTAGAAGATCTTCTGCCATCTAGACCGGAA; GTTCCTAACATAATTGGAGAAACAGAGGAGGTAGAGCTTCAGGAATTTGATAGCACTCGA; GGCTCAGGAGGTGGTCAGAGGCGTGAAGCCTATAATGATAGCTCTGATGAAGAAAGCAGC; AGCCATCATGGACCTGGAGTGCAGTGTGCCCATCAGTAA; ; Clone variation with respect to NM_005880.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005880 unedited; GCATTTGTATACGACTACTATAGGCGGCCGCGAATTCGGCACGAGGCCCCCTCGCTGTCT; CCCTCGGCCTGTGCCGCCGCCGACGCCGCTTGTGGGCCCGACTCCGCTCTGTCTGCTTCG; CCACCTTCTCCCCGAGCACTGCCCGGCCGGCCGCCATGGCTAACGTGGCTGACACGAAGC; TGTACGACATCCTGGGCGTCCCGCCCGGCGCCAGCGAGAACGAGCTGAAGAAGGCATACA; GAAAGTTAGCCAAGGAATATCATCCTGATAAGAATCCAAATGCAGGAGACAAATTTAAAG; AAATAAGTTTTGCATATGAAGTACTATCAAATCCTGAGAAGCGTGAGTTATATGACAGAT; ACGGAGAGCAAGGTCTTCGGGAAGGCAGCGGCGGAGGTGGTGGCATGGATGATATTTTCT; CTCACATTTTTGGTGGGGGATTGTTCGGCTTCATGGGCAATCAGAGTAGAAGTCGAAATG; GCAGAAGAAGAGGAGAGGACATGATGCATCCACTCAAAGTATCTTTAGAAGATCTGTATA; ATGGCAAGACAACCAAACTACAACTTAGCAAGAATGTGCTCTGTAGTGCATGCAGTGGCC; AAGGCGGAAAGTCTGGAGCTGTCCAAAAGTGTAGTGCTTGTCGAGGTCGAGGTGTGCGCA; TCATGATCAGACAGCTGGCTCCAGGGATGGTACAACAGATGCAGTCTGTGTGCTCTGATT; GTAATGGAGAAAGGAGAGGTATTAATGAANAAGACCGCTGTANNAAATGTGAAGGGAAGA; AAGTGATTAAAGAAGTCAAGATTCTTGAGTCCACGTAGACAAGGCACTGAACATGGACAG; AGAANTACATTCACTGGNGAAGCAGACCAGGCCCCAGGAGTGGAACCCGNAGACATGNTC; TTTGCTACAGAGAAAGACATGAGGTATTCANAGAGATGGAATGATNGCCATGACTATANA; ATAGACTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005880 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGGCTATGAA; ATTCTTTATTCTATTTGTAGCAGCTTATGCAGGTGCAGCCAAACACAAAGCTTCAGGACA; AATTGTACAAACTTTACAATGTGGGATTTAAATTTAAAATATGAAACATAAAAATCTACA; CAAAACTGATAAAAATCAAGCACAGATACCAGGATTGAAACTTATAATAATCCATGTGTG; AAAGGGAGTCTTGTTTCCTTTCAAGTGCTTTATTCTGCTATGGAACAGTCAAAATGAAGA; TGTAAAGCTTTGTGGTTAGTTTAAATTATACACTCTGTAGATACTATACCAATTTTAAAA; GTTATACATAGACCAACAGATGTCCCTCAGTTCATCTGGATTGATAAGACACTCCAGCTG; GATTGCTGAGAACAAATCAGGCAAATGTGGAAAGAAAATCCACCTGTGCAATTTGTTTGC; AGAGTTTACTGATGGGCACACTGCACTCCAGGTCCATGATGGCTGCTGCTTTCTTCATCA; GAGCTATCATTATAGGCTTCACGCCTCTGACCACCTCCTGAGCCTCGAGTGCTATCAAAT; TCCTGAAGCTCTACCTCCTCTGTTTCTCCAATTATGTTAGGAACTTCCGGTCTAGATGGC; AGAAGATCTTCTAGTTCAAAAAGCTTGTCTGGGTTGATCCAGTTGTTTTCAGGAAACTGC; CCATCAAACTTTATGTAAAGATCACCTTTTTCAAAAGGGATACGATACTGCGGCATCCCT; TCACTTGAACTACACGAACCACCCTGGGTCAATACTTTGCCAGGGGGTATTTCCCACAAT; CTGACGTCCATCAGGGGCTTAATTGTGACTGTAATCCACTGAGCTTCAACAGTCATTTTA; ATGCATGGCAATCATCCCATTTTTTGAAAACTATGTTTTTCTCTTGACAAGACATGTTTC; CGGTCCCTCTGGGCCGGTTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005880
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005880.2, NP_005871.1
• RefSeq Size: 1993 bp
• RefSeq ORF: 1239 bp
• Locus ID: 10294
• UniProt ID: O60884
• Cytogenetics: 16q11.2
• Domains: DnaJ, DnaJ_C, DnaJ_CXXCXGXG
• Summary: The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]