ACADS (NM_000017) Human Untagged Clone

SKU
SC116331
ACADS (untagged)-Human acyl-CoA dehydrogenase, C-2 to C-3 short chain (ACADS), nuclear gene encoding mitochondrial protein
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Specifications
Product Data
Type Human Untagged Clone
Target Symbol ACADS
Synonyms ACAD3; SCAD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116331 sequence for NM_000017 edited (data generated by NextGen Sequencing)
ATGGCCGCCGCGCTGCTCGCCCGGGCCTCGGGCCCTGCCCGCAGAGCTCTCTGTCCTAGG
GCCTGGCGGCAGTTACACACCATCTACCAGTCTGTGGAACTGCCCGAGACACACCAGATG
TTGCTCCAGACATGCCGGGACTTTGCCGAGAAGGAGTTGTTTCCCATTGCAGCCCAGGTG
GATAAGGAACATCTCTTCCCAGCGGCTCAGGTGAAGAAGATGGGCGGGCTTGGGCTTCTG
GCCATGGACGTGCCCGAGGAGCTTGGCGGTGCTGGCCTCGATTACCTGGCCTACGCCATC
GCCATGGAGGAGATCAGCCGCGGCTGCGCCTCCACCGGAGTCATCATGAGTGTCAACAAC
TCTCTCTACCTGGGGCCCATCTTGAAGTTTGGCTCCAAGGAGCAGAAGCAGGCGTGGGTC
ACGCCTTTCACCAGTGGTGACAAAATTGGCTGCTTTGCCCTCAGCGAACCAGGGAACGGC
AGTGATGCAGGAGCTGCGTCCACCACCGCCCGGGCCGAGGGCGACTCATGGGTTCTGAAT
GGAACCAAAGCCTGGATCACCAATGCCTGGGAGGCTTCGGCTGCCGTGGTCTTTGCCAGC
ACGGACAGAGCCCTGCAAAACAAGAGCATCAGTGCCTTCCTGGTCCCCATGCCAACGCCT
GGGCTCACGTTGGGGAAGAAAGAAGACAAGCTGGGCATCCGGGGCTCATCCACGGCCAAC
CTCATCTTTGAGGACTGTCGCATCCCCAAGGACAGCATCCTGGGGGAGCCAGGGATGGGC
TTCAAGATAGCCATGCAAACCCTGGACATGGGCCGCATCGGCATCGCCTCCCAGGCCCTG
GGCATTGCCCAGACCGCCCTCGATTGTGCTGTGAACTACGCTGAGAATCGCATGGCCTTC
GGGGCGCCCCTCACCAAGCTCCAGGTCATCCAGTTCAAGTTGGCAGACATGGCCCTGGCC
CTGGAGAGTGCCCGGCTGCTGACCTGGCGTGCTGCCATGCTGAAGGATAACAAGAAGCCT
TTCATCAAGGAGGCAGCCATGGCCAAGCTGGCCGCCTCGGAGGCCGCGACCGCCATCAGC
CACCAGGCCATCCAGATCCTGGGCGGCATGGGCTACGTGACAGAGATGCCGGCAGAGCGG
CACTACCGCGACGCCCGCATCACTGAGATCTACGAGGGCACCAGCGAAATCCAGCGGCTG
GTGATCGCCGGGCATCTGCTCAGGAGCTACCGGAGCTGA

Clone variation with respect to NM_000017.2
321 t=>c;625 g=>a;990 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000017 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCCTGGGACTG
TGTCTGTCGCCCATGGCCGCCGCGCTGCTCGCCCGGGCCTCGGGCCCTGCCCGCAGAGCT
CTCTGTCCTAGGGCCTGGCGGCAGTTACACACCATCTACCAGTCTGTGGAACTGCCCGAG
ACACACCAGATGTTGCTCCAGACATGCCGGGACTTTGCCGAGAAGGAGTTGTTTCCCATT
GCAGCCCAGGTGGATAAGGAACATCTCTTCCCAGCGGCTCAGGTGAAGAAGATGGGCGGG
CTTGGGCTTCTGGCCATGGACGTGCCCGAGGAGCTTGGCGGTGCTGGCCTCGATTACCTG
GCCTACGCCATCGCCATGGAGGAGATCAGCCGCGGCTGCGCCTCCACCGGAGTCATCATG
AGTGTCAACAACTCTCTCTACCTGGGGCCCATCTTGAAGTTTGGCTCCAAGGAGCAGAAG
CAGGCGTGGGTCACGCCTTTCACCAGTGGTGACAAAATTGGCTGCTTTGCCCTCAGCGAA
CCAGGGAACGGCAGTGATGCAGGAGCTGCGTCCACCACCGCCCGGGCCGAGGGCGACTCA
TGGGTTCTGAATGGAACCAAAGCCTGGATCACCAATGCCTGNGAGGCTTCGGCTGCCGTG
GTCTTTGCCAGCACGGACAGAGCCCTGCAAAACAAGAGCATCAGTGCCTTCCTGGTCCCC
ATGCCAACGCCTGNGCTCACGTTGGGGAAGAAAGAAGACAAGCTGNGCATCCGGNGCTCA
TCCACGGCCAACCTCATCTTTGAGGACTGTCGCATCCCCAAGGACAGCATCCTGNGGGAG
CCAGGGATGGGCTTTCAGATACCATGCAAACCCTGGACATGGGCCCGCATCGCATCGNCT
TCCCAGCCCTGGNGCATGCCCAGACCGNCCCTCGATGTGCTGTGAACTACGCTGAGAATC
GCATGGGCTTTCGGGGCGCCCNTCACAAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000017 unedited
CCGTCGAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGGGACAGGTGTGTTTATTACCCACA
GTCAGCCACACAGCATGAATCACCACCCCTGCCTTCATCAGACTTTACAAAGGGCTGAGG
GGCCTCTGGGCCAGCTCCATGGCCCAGCCCCACCACCTGCCCAACACCAGGACCCCAGGC
CTCGTCATCCTGACCCCAGTCCTGCTTCTGACCTCTGACCTCAAGGAAAAGACAGACCCC
ACCAAGGGCCTGTAGTACTGGGCCTTAATGCCAGGGCACCAGGGCCCGCGGGAGGACAAC
TGAGGTGTCCCTGAGTGCCGCTCAGCCCCCCCACAACCCCGCCCTCAGGAGGCCAGGGCC
ACTTACATGAGGAACTTGAGGCACAGTGGTGAGGAGGCCGAAAGGCCCATAGGCCGACCT
GCCAACGGTCGGAGGGCCTCATTCCCATGTGATCTGATCTGGGTGGCACAACTGAACCCA
TCTGGGGTACCCAGGGAGCCCCCGACGGGCCGACCATACTCTGACCCGGGGTTGGCGCGG
AGGCCCCTGGCTACCGCGCCTTACCGCACACGTGGGGCAAACCCCCCCCGGCCTCAACTA
CGGTAGCTACTGAGCAAATGCCCGGTGATCACCACACCGCTGGACCACCCTGGTGCCCTT
ATCCACTAAATGATCCGGTCCCACCGGACACGCCCACTCTGTCTGATTCCTTCGCACACT
CAACCCTGCCCCACCAGGAACCTGCAGGCCCTGGTGGCCTAATGTCCGACCCCCGCCAAA
CGAGCGGCACCCCTTGAACTTGGCTGCCTACTTGATACACGCCTACTGTAAACCCTCCAC
ATGCCCCCCCCCCGTAAAAATACCGGGCCCCTCCCGGCCATGACACGGTTGCCACTTGAC
CGGCTAACCGAACCTGGAGAGGGGGCCCCGACGGCCCCCAATCTTCCCTTTACAAGACCA
ATTAGGGGGTTGGGCATGCCCAGGCCGGAGGCACACCACCGCCCC
Restriction Sites NotI-NotI
ACCN NM_000017
Insert Size 1880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000017.1, NP_000008.1
RefSeq Size 1829 bp
RefSeq ORF 1239 bp
Locus ID 35
UniProt ID P16219
Cytogenetics 12q24.31
Domains Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
Protein Families Druggable Genome
Protein Pathways Butanoate metabolism, Fatty acid metabolism, leucine and isoleucine degradation, Metabolic pathways, Valine
Summary This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Citations

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