Nrf2 (NFE2L2) (NM_006164) Human Untagged Clone
SKU
SC116283
NFE2L2 (untagged)-Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1
$564.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Nrf2 |
| Synonyms | HEBP1; IMDDHH; Nrf-2; NRF2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_006164 |
| Insert Size | 1818 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006164.2, NP_006155.2 |
| RefSeq Size | 2439 bp |
| RefSeq ORF | 1818 bp |
| Locus ID | 4780 |
| UniProt ID | Q16236 |
| Cytogenetics | 2q31.2 |
| Domains | BRLZ |
| Protein Families | Transcription Factors |
| Summary | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. provided by RefSeq, Sep 2015 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006164" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204140 | NFE2L2 (Myc-DDK-tagged)-Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1 | 10 ug |
$563.00
|
|
| RC204140L1 | Lenti ORF clone of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$863.00
|
|
| RC204140L2 | Lenti ORF clone of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, mGFP tagged | 10 ug |
$863.00
|
|
| RC204140L3 | Lenti ORF clone of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$863.00
|
|
| RC204140L4 | Lenti ORF clone of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, mGFP tagged | 10 ug |
$863.00
|
|
| RG204140 | NFE2L2 (tGFP-tagged) - Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1 | 10 ug |
$763.00
|
|
Citations
NFE2L2 and SLC25A39 drive cuproptosis resistance through GSH metabolism
Liu, Tang, Chen et al
Sci Rep (2024) 14 (1), 29579 DOI: 10.1038/s41598-024-81317-x
Liu, Tang, Chen et al
Sci Rep (2024) 14 (1), 29579 DOI: 10.1038/s41598-024-81317-x
Cited by 46
AI Summary: OriGene products contributed to this study by providing human NFE2L2 cDNA (SC116283), which was used for transfection experiments to investigate the role of NFE2L2 in cuproptosis resistance.
Snippet: 026594), human SLC25A39#1 (HSPD0000087762), human SLC25A39#2 (HSPD0000087764), mouse Slc25a39#1 (MMPD0000071924), and mouse Slc25a39#2 (MMPD0000071925). Human NFE2L2 cDNA (SC116283) was acquired from OriGene and transfected using Lipofectamine 3000 (Thermo Fisher Scientific) as per the manufacturer’s guidelines38. Western blot Western blotting was carried out as described previously20,39,40. In
Ferroptosis is induced by lenvatinib through fibroblast growth factor receptor-4 inhibition in hepatocellular carcinoma
Iseda, Itoh, Toshida et al
Cancer Sci (2022) 113 (7), 2272-2287 DOI: 10.1111/cas.15378
Iseda, Itoh, Toshida et al
Cancer Sci (2022) 113 (7), 2272-2287 DOI: 10.1111/cas.15378
Cited by 113
AI Summary: In this study, OriGene’s Nrf2 plasmid (cat. SC116283, NM_006164) was used to overexpress Nrf2 in hepatocellular carcinoma cells, and OriGene’s empty vector (cat. PS100063, pCMV6‐Entry Vector) served as a control, enabling the authors to assess the role of Nrf2 in lenvatinib-induced ferroptosis.
Snippet: d‐derived 2‐like 2 overexpressing cells and nuclear factor erythroid‐derived 2‐like 2‐ or fibroblast growth factor receptor‐4‐suppressed cells An Nrf2 plasmid (cat. SC116283, NM_006164;Origene) was transfected into HCC cells using a jetPRIME kit (ct. 114, Polyplus Transfection). An empty vector (cat. PS100063, pCMV6‐Entry Vector;Origene) was also transfected into HCC cells as a c
Impact of Nuclear Factor Erythroid 2-Related Factor 2 in Hepatocellular Carcinoma: Cancer Metabolism and Immune Status
Iseda, Itoh, Yoshizumi et al
Hepatol Commun (2021)
Iseda, Itoh, Yoshizumi et al
Hepatol Commun (2021)
Cited by 18
AI Summary: OriGene products, specifically the NRF2 plasmid (NM_006164) and the pCMV6Entry Vector, were used to manipulate NRF2 expression in hepatocellular carcinoma cell lines, enabling the study of NRF2’s role in cancer metabolism and immune regulation in this research.
Snippet: NRF2 plasmid (NM_006164;Origene) was transfected into HCC cells using the jetPRIME kit (Polyplus Transfection). An empty vector (pCMV6Entry Vector;Origene) was also transfected into HCC cells as a control. Forty- eight hours after transfection, HCC cells Save;- NRF2 plasmid (NM_006164;Origene) was also transfected into HCC cells as a control. Forty- eight hours after transfection, HCC cells
Activation of the Nrf2-ARE Signaling Pathway Prevents Hyperphosphatemia-Induced Vascular Calcification by Inducing Autophagy in Renal Vascular Smooth Muscle Cells
Yao, Wang, Tian et al
J. Cell. Biochem. (2017)
Yao, Wang, Tian et al
J. Cell. Biochem. (2017)
Dysregulated autophagy increased melanocyte sensitivity to H2O2-induced oxidative stress in vitiligo
He, Li, Zhang et al
Sci Rep (2017) 7, 42394 DOI: 10.1038/srep42394
He, Li, Zhang et al
Sci Rep (2017) 7, 42394 DOI: 10.1038/srep42394
AI Summary: OriGene’s pCMV6-XL5-Nrf2 plasmid was used to overexpress Nrf2 in melanocytes, helping the researchers study how enhancing the Nrf2-p62 pathway affects autophagy and oxidative stress sensitivity in vitiligo.
Snippet: Cells were seeded at 2 × 10 5 cells per well for 24 hours before transfection. Cells were transfected with pCMV6-XL5-Nrf2 (OriGene, MD, USA) or pCMV-MCS-p62 (Genechem, Shanghai, China) at 4 mg per well for 6-well plates by Lipofectamine 3000 (Invitrogen). ... All 4 versions Cite SaveSaving
An oxidative stress mechanism of shikonin in human glioma cells
Yang, Li, Wu et al
PLoS ONE (2014) 9 (4), e94180 DOI: 10.1371/journal.pone.0094180
Yang, Li, Wu et al
PLoS ONE (2014) 9 (4), e94180 DOI: 10.1371/journal.pone.0094180
Cited by 80
AI Summary: In this study, the pCMV6-XL5 expression vector from OriGene was used to overexpress Nrf2 in glioma cells, enabling the researchers to investigate the role of Nrf2 in shikonin-induced oxidative stress and apoptosis.
Snippet: Overexpression of Nrf2 in U87MG cells. Full-length nuclear factor-erythroid 2 p45-related factor (Nrf2) cDNA was generated by polymerase chain reaction (PCR) and subcloned into the pCMV6-XL5 expression vector (OriGene Technologies Inc., Rockville, MD). ... Cite SaveSaving
Showing 1-6 of 15 papers.
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