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TFPI (NM_006287) Human Untagged Clone

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SKU
SC116194
TFPI (untagged)-Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TFPI
Synonyms EPI; LACI; TFI; TFPI1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116194 sequence for NM_006287 edited (data generated by NextGen Sequencing)
ATGATTTACACAATGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCTGCTGCTTAAT
CTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACACAATTATCACA
GATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAAGGCGGATGAT
GGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCGACAGTGCGAA
GAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCTGGAAGAGTGC
AAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATTGCAACAAGAA
AAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTATATTACCAGG
TATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTCAAGTATGGTGGATGCCTGGGC
AATATGAACAATTTTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGATGGTCCGAAT
GGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTCCCTGACTCCG
CAATCAACCAAGGTTCCCAGCCTTTTTGAATTTCACGGTCCCTCATGGTGTCTCACTCCA
GCAGACAGAGGATTGTGTCGTGCCAATGAGAACAGATTCTACTACAATTCAGTCATTGGG
AAATGCCGCCCATTTAAGTACAGTGGATGTGGGGGAAATGAAAACAATTTTACTTCCAAA
CAAGAATGTCTGAGGGCATGTAAAAAAGGTTTCATCCAAAGAATATCAAAAGGAGGCCTA
ATTAAAACCAAAAGAAAAAGAAAGAAGCAGAGAGTGAAAATAGCATATGAAGAAATTTTT
GTTAAAAATATGTGA

Clone variation with respect to NM_006287.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006287 unedited
GTCAGGATTTGNTATACGACTTTACTATAGGGCGGCCGCGAATTCGCACCAGGGTTAGCA
GCACAAGAGATGATTTACACAATGGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCT
GCTGCTTAATCTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACAC
AATTATCACAGATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAA
GGCGGATGATGGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCG
ACAGTGCGAAGAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCT
GGAAGAGTGCAAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATT
GCAACAAGAAAAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTA
TATTACCAGGTATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTCAAGTATGGTGG
ATGCCTGNGCAATATGAACAATTNTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGA
TGGTCCGAATGGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTC
CCTGACTNCGCANTCAACCCAAGTTCCCAGCCTTTTTGAATTTCACGGTCCCTCATGGTG
TCTCACTCCAGCAGACAGAAGATTGTGTCGTGNCAATGAGAACAGATTCTACTANCATTC
AGTCATTGGGAAATGCCGNCCATTTAAGTACAGTGGATGTGGGGGAAATGAAAACANATT
TACTTCCAACCAGAATGTCTGAGGGCATGTAAAAAAGTTTCANTCCAAGAATATCAAAGG
GAGGCCTATTTANACCAAAGAAAAAGAAGAAGCCAGAGTGAAATAGCTATGAAGAAATTT
TTGTTAAATATGT
Restriction Sites NotI-NotI |
ACCN NM_006287
Insert Size 3750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006287.3, NP_006278.1
RefSeq Size 1431 bp
RefSeq ORF 915 bp
Locus ID 7035
UniProt ID P10646
Cytogenetics 2q32.1
Domains KU
Protein Families Secreted Protein
Protein Pathways Complement and coagulation cascades
Summary This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. provided by RefSeq, Jul 2016
Transcript Variant: This variant (1) encodes the longer isoform (a, also known as TFPIalpha), which is secreted. Variants 1 and 4-6 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_006287" in other vectors (6)
SKU Description Size Price
RC219033 TFPI (Myc-DDK-tagged)-Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1 10 ug
$300.00
RC219033L1 Lenti ORF clone of Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC219033L2 Lenti ORF clone of Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1, mGFP tagged 10 ug
$600.00
RC219033L3 Lenti ORF clone of Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC219033L4 Lenti ORF clone of Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1, mGFP tagged 10 ug
$600.00
RG219033 TFPI (tGFP-tagged) - Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1 10 ug
$489.00 $500.00

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