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VTI1B (NM_006370) Human Untagged Clone

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SKU
SC116155
VTI1B (untagged)-Human vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B)
  $300.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VTI1B
Synonyms v-SNARE; VTI1; VTI1-LIKE; vti1-rp1; VTI1L; VTI2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC116155 representing NM_006370.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCGCGGCTCCGGAAGAGCCTCGT
CCTGGGCGGCGGTGGTGCGGCGGTCGCCGTTATGGCCACTGGGCTGGGCGGCTGACCGCCGGGCTAGGA
AAGGGCCCAGGGCCCCGAATCTCGGTGGCCGCTGCTCCAGCGCGGCCTGCGCCATGGCCTCCTCCGCCG
CCTCCTCGGAGCATTTCGAGAAGCTGCACGAGATCTTCCGCGGCCTCCATGAAGACCTACAAGGGGTGC
CCGAGCGGCTGCTGGGGACGGCGGGGACCGAAGAAAAGAAGAAATTGATCAGGGATTTTGATGAAAAGC
AACAGGAAGCAAATGAAACGCTGGCAGAGATGGAGGAGGAGCTACGTTATGCACCCCTGTCTTTCCGAA
ACCCCATGATGTCTAAGCTTCGAAACTACCGGAAGGACCTTGCTAAACTCCATCGGGAGGTGAGAAGCA
CACCTTTGACAGCCACACCTGGAGGCCGAGGAGACATGAAATATGGCATATATGCTGTAGAGAATGAGC
ATATGAATCGGCTACAGTCTCAAAGGGCAATGCTTCTGCAGGGCACTGAAAGCCTGAACCGGGCCACCC
AAAGTATTGAACGTTCTCATCGGATTGCCACAGAGACTGACCAGATTGGCTCAGAAATCATAGAAGAGC
TGGGGGAACAACGAGACCAGTTAGAACGTACCAAGAGTAGACTGGTAAACACAAGTGAAAACTTGAGCA
AAAGTCGGAAGATTCTCCGTTCAATGTCCAGAAAAGTGACAACCAACAAGCTGCTGCTTTCCATTATCA
TCTTACTGGAGCTCGCCATCCTGGGAGGCCTAGTTTACTACAAATTCTTTCGCAGCCATTGAACTTCTA
TAGGGAAGGGTTTGTGGACCAGAACTTTGACCTTGTGAATGCATGATGTTAGGGATGTGGATAGAATAA
GCATATTGCTGCTGTGGGCTGACAGTTCAAGGATGCACTGTATAGCCAGGCTGTGGGAGGAGGGAGGAA
AGATGAAAAACCACTTAAATGTGAAGGAACAACAGCAACAAGACCAGTATGATATACCAAGGTAATAAA
TGCTGTTTATGACTTCTTTAAATTTACATAGTACTGTAGCATATTAATACCCTGTGAACTGCAAAAAAC
CAAATACATTTACAGTAGTATTGGTCACCAAAATAGAGGGGAAACTTTACAATTGTGAGAATGTGTAAA
TGTTCTCATTAAGGCAGTATTGACCCAGACAACCATTTAGTATTCATCTATCCCCTCAATGCCTCATAA
TTCTGGAATGCCTGTTGTGAAACATGTCAGTGCACAGTGTCTCCTAAATTCTCACACGTGCTTGATTTT
CTGATTCATCTGGTGAACTGGGAGTAGGAAGTTGGTCATAGACAATATGCCCTCCTTCTCTTGTCTGAC
CAAAGCTTGAAGCAATCACATCTACTGCCAGGTTAGCTGTAGTCTTCGCCTCTTCCTCTGAGGTGGCCA
ACTGAGGATTGACTTCAACAAGATCCAGTGCTGATAGCAACCCTGGAAGAACGAAGTGTGACAAAACCT
CAGGTTCCCTTGCTGCTACTCTCAGTGAGGGTCATCCCACTGGGACAGGGAGAACAAGCCAAAGTAAAA
ACAAGAGTCCATTTTATAGTAGAAAATACCTATTTTTAGGAAGCCCCTTGCACCTCATCTTGGCCATGA
ATTTAAGTTAAAACACTGTTGTGCTATAGTAGATTAAAAGAAACCTTTTAAGATAATGAAATAAACCAT
CCCTGTTCAACTAACATAACAGGCATAATTGTCAGGCGTTCTGGAAAAGGAAAAGGCTTGGTGTTGGGT
GGGGGAGGTTTTCATCCTGCTTGTTGGCAAAATGTATGCCACCTCCAGTATGACTAGGAACCTTCCAGA
TGCCTGCCTGGAAATGAATCAAGTATGAAGGCAGAGGAAATCTGCCACTTTCACTAGCCAAACTGCATT
CCCACTTTTGAACTCTAATGTGTTATCTGTATCTGTTAGAGCACCACACCATATGCCCTGAAATGTAGT
TATTACTGCAAACTTCATTCTCACCCCTGCAGGCCTGAGCTCTGGAGGCAGGTTCCACATCTAACAACA
TCTTTTTGTCTCCCAGCAGACTGACCCAGTGTCTTGTACTTGATCAAGAGAAAATTCAGCCATATGTTA
ATTATTTTATCTGGACTTCAGTTACCTTCTGTTAAGGTTTGAGAACTTGTGTCAAAGTTCTCGGTAGTG
TTCCTTTGGCCTGGAGCAAGTGGAAGGGAAGCTTAAACCAGAAGAACCTATCCAGACCTGCTACTATGC
TCACCCCTCTAAATGCCTCACTTCTCTATAGAATTTAACTTTTTAGTTGGTTATTCTGGATGAAGGGTG
ATCTGAACAAGCTAGTATTCAGGAATACAAGTATCTAAGAATATTTAGGGTGGCCATAAAGTCTGGAAA
CACAGGCAAATGTACCTAATGGTTTATTGATATTACACTATAGTACATGATGTGCTGAATGCCATCATG
TCCGTTCAGCATCTTCCCTTATTAGCAATGCATAGTTGAGTGTACAGTGCCAAACTGCAACCAACAGGT
GTATTACTGTAGCATTTCCATCATTTCCTGCACATGGACCTGTGTTCCTGATTTGTCTCTGATTTCCAC
TGACTAAACCTGCACCTTTAGCATATCCCAGAAGTGGTCATCAAAGTTTGCACTATTAGAAATATATAC
ATGACTTATGTTTCCAGACTATTGCCACCCTATAAATTAAGCAAACTGCTTTATGTATCTAGTTGACAG
CATTATAACAGTGCAGTGGCACAATCATGGCTCACTGCAGCCTCAACCTCCCAAGTAGTTGGGACACCT
GGCTAATTATTTTTTGTAGAAACGGGGTCACACTATGTTGCCCACGCTGGTCTTGAACCCCTGGGGTCA
AGTAATCCTCTTGCCTTGGCCTCCCAGAGTACTGGGATTATAGGTATGAACCACTGTGCCTAGCCCCAT
GAGTGTCTTTTGGAGTAAAATATTGTAGCATAGGAACAAAGAGACAAGCCATGCCATATATAAGTGCTA
GAGGTTATTAACCCCTGCTCTAAGTGGTGTTTTTAGGAAGCAAAGCAGAGAATTGAACCCCCAAGTTCT
CTGGCCAATAACCTAAGGACCACTGGCAACATCTAAGATTGCAGATTCTGAGAATTAGACCAGTACTGC
CTGCCTTTTTGTACTGCCTAAGGTTCTGGCAGGCTGTGGCGCTAAGGAGACCATTTCTCAATGTGAGTT
TCTATCCCAGCTAGTCAAGGGTTCTCTATAGCCCTGAACCTTTCAATCTGGGGTCTCTATCTATACCCT
AGTTCCATCAGATGGTTACTGAAGCAGTATGATTTGGTAGTTCTTTAAATTTTGGAATGAAAGGTCATT
TGAAGTAGCTTTAAAACCAGAAGCTTTCTGACAGAAATGGAAACTGTTTGCAAACAAGTATGAGTTTTG
GAAAAGTAGCCAGTCTTGATAACGTAGAATTCATCTTAGTACTCTTTCCATCAGCCATTCTCATCTAAC
TGTATCCCCCTTATTAGAAATCCTTTCAGGCTCTAGTACAAGACCTACCCTACATAAAGGACCCAAGAT
ATTGTCCAGTTTGCAGATTTCTTCAAGAGATCAGCTTTAGCTCCAAATTCACAACTCCCAATGAACCAA
TCTCTTAGAGTGCAAATTACAGCCTTGCACTCTTATCTTATCTTGCCTTCTTATCTTAATCTGCCTTTA
GATAACTCAAAGAACTGCATCAGTTTGGCAATTTATTCTTGTATTTAATTGTTTAAATATACAATTTTT
TACTCATTATTACCTTAGTGTAATTTGGTAAGAGCCATGTCCAAGCATGACTCTAAAGGACTATATCCT
TTTTATTAGCATGGCCATATAATTTTTAGCTACAAAAGTTTAAGCAAAAACAAACTCTGCAATGATGTG
GAATAGCAGAAAAGATCCTTTGCAGGCAAGAGACTCTAGCATATTTACCTATGTAGTTAACAGGCTGCA
GACCTGGTTGCTACATACCTGTATTGTGTATTTCCTCAGCAATATACATGCCTTCTCGATAGGTTAGTC
CCCCGACAACAGGAGTTCCTGTGGCTGGAGCCAGTGTAGGGTCAAATGCATCAATATCAAAACTCAAAT
GGATTGGTCTTTGTCTCCTAAAAAGGAAGAGGATAAAATAATACTTAAAATAATACTTAACTGGTTGTC
CTTGGACACTCATCCTTCTTATGGTTGCAACTCCTTGATTTTGTTGCTATTAGTCCTAGTACTTTGTTA
ATAAAGCTGTTAATACATTTCCAGGCTTCCTATTCTTGGAAGTTACTGAGATTGGCAATATTAACTTCC
TACATACCACTTCATGATACTGCCATTTGTTCTGCATACCTCAGACAGACTAACTCCAATGTATATTTC
TGCCTTACACTGGGTATTAGAACCTCTTATACTAATAACTGTCAATAGAGAGATTTCTTCACTTCTAGA
TGAGATTGGGCTCTCATCTAGAGACAATTTAATTAGACAATTTAAATTAGTCTAATTTAAATTGGCTTG
ATGTAAGTTATGGTTGCTCTTATTCTCTGAACGTAACAGCAGCTGAAGTGAACTCTAATTTTAGGTCTG
CAGTTTTAACATCTTCCATTTCTAACTTCATTATAGCCCCAGTGTCCAAAGGCTGAAGCTAACAACTAA
GTTTCTTAACAGCTTATGAAATGACATACTACCAGATAACAATTCTGACATTAATCACCCAGAAGTAAA
GACTTGGTATTGGGAAAATGCTCTTAAAATTACCATTTGATTAGTATTCATCTGCCATCTTTCTACACT
GCCAAGATTTGCTTAATATAAAACTCAGATGAAACAATCGTGAGGCTGGGAAGAATGGGACAGTTGAAA
CAATAAATGCTTGTCATCTCAGAAGATAAGCATTAAAGATGGAGATAAAGAAAAATTCATGAGACAGAA
GATCTAGGCAATGATTTAGAGGAAACCTCCTCCCAAAAACCCCAGAGGAAGAATATGACCTGTGTTTCA
AATTTTGCCTTTCTGAACTATTTAAAATTATGTGTATGTTTTTGCTTTGTATTTTAAAAGTAAAGCTGC
TGTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006370 unedited
CACGAGGCCTCGTGCCGAATTCGGCACGAGGCGCGGCTCCGGNAAGAGCCTCGTCCTGGG
CGGCGGTGGTGCGGCGGTCGCCGTTATGGCCACTGGGCTGGGCGGCTGACCGCCGGGCTA
GGAAAGGGCCCAGGGCCCCGAATCTCGGTGGCCGCTGCTCCAGCGCGGCCTGCGCCATGG
CCTCCTCCGCCGCCTCCTCGGAGCATTTCGAGAAGCTGCACGAGATCTTCCGCGGCCTCC
ATGAAGACCTACAAGGGGTGCCCGAGCGGCTGCTGGGGACGGCGGGGACCGAAGAAAAGA
AGAAATTGATCAGGGATTTTGATGAAAAGCAACAGGAAGCAAATGAAACGCTGGCAGAGA
TGGAGGAGGAGCTACGTTATGCACCCCTGTCTTTCCGAAACCCCATGATGTCTAAGCTTC
GAAACTACCGGAAGGACCTTGCTAAACTCCATCGGGAGGTGAGAAGCACACCTTTGACAG
CCACACCTGGAGGCCGAGGAGACATGAAATATGGCATATATGCTGTAGAGAATGAGCATA
TGAATCGGCTACAGTCTCAAAGGGCAATGCTTCTGCAGGGCACTGAAAGCCTGAACCGGG
CCACCCAAAGTATTGAACGTTCTCATCGGATTGCCACAGAGACTGACCAGATTGGCTCAG
AAATCATAGAAGAGCTGGGGGAACAACGAGACCAGTTAGAACGTACCAAGAGTAGACTGG
TAAACACAAGTGAAAACTTGAGCAAAGTCGGAAGATTCTNCGTTCAATGTCCAGAAAAGT
GACAACCAACAAGCTGCTGCTTTNCATTATCATCTTACTGGAGCTCGC
Restriction Sites NotI-NotI |
ACCN NM_006370
Insert Size 5235 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006370.1
RefSeq Size 1287 bp
RefSeq ORF 5235 bp
Locus ID 10490
UniProt ID Q9UEU0
Cytogenetics 14q24.1
Domains t_SNARE, V-SNARE
Protein Families Druggable Genome, Transmembrane
Protein Pathways SNARE interactions in vesicular transport
MW 198.3 kDa
Summary V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_006370" in other vectors (4)
SKU Description Size Price
RC201172 VTI1B (Myc-DDK-tagged)-Human vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B) 10 ug
$300.00
RC201172L3 Lenti ORF clone of Human vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B), Myc-DDK-tagged 10 ug
$600.00
RC201172L4 Lenti ORF clone of Human vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B), mGFP tagged 10 ug
$600.00
RG201172 VTI1B (tGFP-tagged) - Human vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B) 10 ug
$489.00 $500.00

file-alt Citations

Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator
Cheng, Cebotaru, Cebotaru et al
Mol Biol Cell (2010) 21 (7), 1178-87 DOI: 10.1091/mbc.e09-03-0229
Cited by 66
AI Summary: In this study, OriGene products—specifically the CAL (SC119447) and Syntaxin 6 (STX6, SC119634) expression plasmids—were used to investigate how these proteins interact to regulate the degradation and stability of the cystic fibrosis transmembrane conductance regulator (CFTR).
FigureFigureFigure
Showing 1-1 of 1 papers.
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