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UBE2E3 (NM_006357) Human Untagged Clone

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SKU
SC116144
UBE2E3 (untagged)-Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1
  $450.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UBE2E3
Synonyms UBCH9; UbcM2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116144 sequence for NM_006357 edited (data generated by NextGen Sequencing)
ATGTCCAGTGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCACCAGCAGTGGCAGTTCA
GATGCGGACCAGCGAGACCCAGCCGCTCCAGAGCCTGAAGAACAAGAGGAAAGAAAACCT
TCTGCCACCCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCC
ACTAGTGCTAAAAGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAAT
TGCAGTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTTGGTCCA
CCGGGTTCTGTATATGAAGGTGGTGTGTTTTTTCTGGATATCACATTTTCATCAGATTAT
CCATTTAAGCCACCAAAGGTTACTTTCCGCACCAGAATCTATCACTGCAACATCAACAGT
CAGGGAGTCATCTGTCTGGACATCCTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCA
AAGGTTTTGCTGTCTATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTT
GGAAGCATAGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCCAGACAG
TGGACCAAGAGATACGCAACATAA

Clone variation with respect to NM_006357.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006357 unedited
TCAAATTTTGTAATACGACTTCCTATAGGGGCGGCCGCGATTCGGCACGAGGTAGCGGCG
CGCGAGCGGGCCGGGCGGGCGGCCGAGTTTTCCAAGAGATAACTTCACCAAGATGTCCAG
TGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCACCAGCAGTGGCAGTTCAGATGCGGA
CCAGCGAGACCCAGCCGCTCCAGAGCCTGAAGAACAAGAGGAAAGAAAACCTTCTGCCAC
CCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCCACTAGTGC
TAAAAGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAATTGCAGTGC
TGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTTGGTCCACCGGGTTC
TGTATATGAAGGTGGTGTGTTTTTTCTGGATATCACATTTTCATCAGATTATCCATTTAA
GCCACCAAAGGTTACTTTCCGCACCAGAATCTATCACTGCAACATCAACAGTCAGGGAGT
CATCTGTCTGGACATCCTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCAAAGGTTTT
GCTGTCTATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTTGGAAGCAT
AGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCCAGACAGTGGACCAA
GAGATACGCAACATAATTCACATAATTTGTATGCAGTGTGAAGGAGCAGAAAGCATCTTC
TCACTGTGCTGCANATCTTTATAGCCTTTNACATACGGACTTCTGNGGATATGGTATACT
GGATCTACTCTGGCTTTATCCCTTTGGAGCCTGGGGAGACTTCCAAAAAGGTAAATGCTA
TNCAGAGTAGAACTTTGTAGCTGTAGATAAGTATAGTTAAAACGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006357 unedited
CAATCTAGAGTCGAGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTCAGGACCATGTTTTATTGGGTTTGAAAATAATACATAAGGGGCTTTTTGACC
TACAAAGGGTAAAAGGAAATCCTTTCTTTACAAACTGACAAAGGTAAAAGGTAAAACTTT
TTTACCAACAGCTTGACCCACATACTCAAAAATAAATCTGGGTTAAAAACAAAGGACATA
TTTCCTACCCATCTACAGTTACTTTTATAACTTCATACAAATAATGAATTAGGCTAAAAG
GCCCTATAATATTGGGAAAACTTAAGGACCAGTTTCCTTAGTACATCCCAAAATACTTTT
TGATATCCCAAAAAACCAAAACTTGCAAGTAGGCGTTTAAAACATAACTAATCTACAGTT
ACAAAGTTCTACTCTTGATACCATTAACCTTTTTGGGGAGTCTCCCAGGCTCCAAGGGAT
AAAACCAAAGTAAAATCAGTTTAACCATTTCCCCAAAAGTCCGTATTGAAAAGCCTATAA
AGATTTGCACCACAGTGAGAAGATCCCTTCTGCTCCTTCACACTGAATACAAATAATGGG
AATTATGTGGCGTATCTTTTGGCCCACTGTCGGGCTATCCTGCGCGGTCCTGCCCTGTTG
GAAAAAACCGAGGGGTTATGCTTCCAACCAAAGGATCCCAAGTTTCCATTCTGCCAAAAG
GAAACAATAAACACAAAACCTTTGAAAAATCAAAGCGGCCCTCCGTGTTTTTAAGATGCC
ACAAAAAAACCCCTGCATGTGAGGTTCAAGAATAATCTGGGGCGAAAACAACCTTGGCGG
CTAAAAGGAACTCCGGAAAAGTGGATTCCAAAAAACCCCCCCTTTCATTAACAACCCGGG
GCACAAAAANTAACCCCCTCAAAAGGTCCCCCCTAGCCCACCCGCCATAAGAGAGAAACA
GGGTTTCCCTCCCCTTCGAATCTTAACCAAGGAAACAACAGGGTT
Restriction Sites NotI-NotI |
ACCN NM_006357
Insert Size 1350 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006357.2, NP_006348.1
RefSeq Size 1559 bp
RefSeq ORF 624 bp
Locus ID 10477
UniProt ID Q969T4
Cytogenetics 2q31.3
Domains UBCc
Protein Pathways Ubiquitin mediated proteolysis
Summary The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jun 2013
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same protein.
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Documents
Resources
"NM_006357" in other vectors (6)
SKU Description Size Price
RC210328 UBE2E3 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1 10 ug
$450.00
RC210328L1 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC210328L2 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, mGFP tagged 10 ug
$750.00
RC210328L3 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC210328L4 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, mGFP tagged 10 ug
$750.00
RG210328 UBE2E3 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1 10 ug
$650.00

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