MTHFS (NM_006441) Human Untagged Clone

SKU SC116123

MTHFS (untagged)-Human 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (MTHFS), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: MTHFS
• Synonyms: HsT19268; NEDMEHM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006441, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGGCAGCGGTGAGCAGCGCCAAGCGGAGCCTGCGGGGAGAGCTGAAGCAGCGTCTGCGGGCGA; TGAGTGCCGAGGAGCGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGTGATTGCCCACAGTGAGTATCA; AAAGTCCAAAAGAATTTCCATCTTTCTGAGCATGCAAGATGAAATTGAGACAGAAGAGATCATCAAGGAC; ATTTTCCAACGAGGCAAAATCTGCTTCATCCCTCGGTACCGGTTCCAGAGCAATCACATGGATATGGTGA; GAATAGAATCACCAGAGGAAATTTCTTTACTTCCCAAAACATCCTGGAATATCCCTCAGCCTGGTGAGGG; TGATGTTCGGGAGGAGGCCTTGTCCACAGGGGGACTTGATCTCATCTTCATGCCAGGCCTTGGGTTTGAC; AAACATGGCAACCGACTGGGGAGGGGCAAGGGCTACTATGATGCCTATCTGAAGCGCTGTTTGCAGCATC; AGGAAGTGAAGCCCTACACCCTGGCGTTGGCTTTCAAAGAACAGATTTGCCTCCAGGTCCCAGTGAATGA; AAACGACATGAAGGTAGATGAAGTCCTTTACGAAGACTCGTCAACAGCTTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006441 unedited; GTAACGTTCAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGCAGCG; GTGAGCAGCGCCAAGCGGAGCCTGCGGGGAGAGCTGAAGCAGCGTCTGCGGGCGATGAGT; GCCGAGGAGCGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGTGCGAGGCCGCCCGTAG; CGGAAGCCGCGGCGGACAGACCCTCCGAAGGCCTGGCGGCCAGCGATTGCTGATCTGTGC; ATGGTGATTGCCCACAGTGAGTATCAAAAGTCCAAAAGAATTTCCATCTTTCTGAGCATG; CAAGATGAAATTGAGACAGAAGAGATCATCAAGGACATTTTCCAACGAGGCAAAATCTGC; TTCATCCCTCGGTACCGGTTCCAGAGCAATCACATGGATATGGTGAGAATAGAATCACCA; GAGGAAATTTCTTTACTTCCCAAAACATCCTGGAATATCCCTCAGCCTGGTGAGGGTGAT; GTTCGGGAGGAGGCCTTGTCCACAGGGGGACTTGATCTCATCTTCATGCCAGGCCTTGGG; TTTGACAAACATGGCAACCGACTGGGGAGGGGCAAGGGCTACTATGATGCCTATCTGAAG; CGCTGTTTGCAGCATCAGGAAGTGAAGCCCTACACCCTGGCGTTGGCTTTCAAAGAACAG; ATTTGCCTCCAGGTCCCAGTGAATGAAAACGACATGAAGGTAGATGAAGTCCTTTACGAA; GACTCGTCAACAGCTTAAATCTGGATTACTACAGCCAAATAATCAGTGTTTTATATGAGA; GTAAAGCANAGTATGTGTATTTTTTCCCTTGTCAAAAATAGTTGAAATTGTTTCATAATG; TGAATACAGACTGCATTTTAAAATTGTTATTATGAAAATACTTATATAAAACCATCTTTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_006441
• Insert Size: 970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006441.1, NP_006432.1
• RefSeq Size: 857 bp
• RefSeq ORF: 612 bp
• Locus ID: 10588
• UniProt ID: P49914
• Cytogenetics: 15q25.1
• Domains: 5-FTHF_cyc-lig
• Protein Pathways: Metabolic pathways, One carbon pool by folate
• Summary: The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.