SLC35A1 (NM_006416) Human Untagged Clone

SKU SC116104

SLC35A1 (untagged)-Human solute carrier family 35 (CMP-sialic acid transporter), member A1 (SLC35A1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLC35A1
• Synonyms: CDG2F; CMPST; CST; hCST
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116104 sequence for NM_006416 edited (data generated by NextGen Sequencing); ATGGCTGCCCCGAGAGACAATGTCACTTTATTATTCAAGTTATACTGCTTGGCAGTGATG; ACCCTGATGGCTGCAGTCTATACCATAGCTTTAAGATACACAAGGACATCAGACAAAGAA; CTCTACTTTTCAACCACAGCCGTGTGTATCACAGAAGTTATAAAGTTATTGCTAAGTGTG; GGAATTTTAGCTAAAGAAACTGGTAGTCTGGGTAGATTCAAAGCATCTTTAAGAGAAAAT; GTCTTGGGGAGCCCCAAGGAACTGTTGAAGTTAAGTGTGCCATCGTTAGTGTATGCTGTT; CAGAACAACATGGCTTTCCTAGCTCTTAGCAATCTGGATGCAGCAGTGTACCAGGTGACC; TACCAGTTGAAGATTCCGTGTACTGCTTTATGCACTGTTTTAATGTTAAACCGGACACTC; AGCAAATTACAGTGGGTTTCAGTTTTTATGCTGTGTGCTGGAGTTACGCTTGTACAGTGG; AAACCAGCCCAAGCTACAAAAGTGGTGGTGGAACAAAATCCATTATTAGGGTTTGGCGCT; ATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGGAGTATATTTTGAAAAAGTTTTAAAG; AGTTCAGATACTTCTCTTTGGGTGAGAAACATTCAAATGTATCTATCAGGGATTATTGTG; ACATTAGCTGGCGTCTACTTGTCAGATGGAGCTGAAATTAAAGAAAAAGGATTTTTCTAT; GGTTACACATATTATGTCTGGTTTGTCATCTTTCTTGCAAGTGTTGGTGGCCTCTACACT; TCTGTTGTGGTTAAGTACACAGACAACATCATGAAAGGCTTTTCTGCAGCAGCGGCCATT; GTCCTTTCCACCATTGCTTCAGTAATGCTGTTTGGATTACAGATAACACTCACCTTTGCC; CTGGGTACTCTTCTTGTATGTGTTTCCATATATCTCTATGGATTACCCAGACAAGACACT; ACATCCATCCAACAAGGAGAAACAGCTTCAAAGGAGAGAGTTATTGGTGTGTGA; ; Clone variation with respect to NM_006416.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006416 unedited; GGTTCAGATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGTCGGGGAA; CCTGGCTGCCCCGAGNAAAAATGTCACTTTATTATTCAAGTTATACTGCTTGGCAGTGAT; GACCCTGATGGCTGCAGTCTATACCATAGCTTTAAGATACACAAGGACATCAGACAAAGA; ACTCTACTTTTCAACCACAGCCGTGTGTATCACAGAAGTTATAAAGTTATTGCTAAGTGT; GGGAATTTTAGCTAAAGAAACTGGTAGTCTGGGTAGATTCAAAGCATCTTTAAGAGAAAA; TGTCTTGGGGAGCCCCAAGGAACTGTTGAAGTTAAGTGTGCCATCGTTAGTGTATGCTGT; TCAGAACAACATGGCTTTCCTAGCTCTTAGCAATCTGGATGCAGCAGTGTACCAGGTGAC; CTACCAGTTGAAGATTCCGTGTACTGCTTTATGCACTGTTTTAATGTTAAACCGGACACT; CAGCAAATTACAGTGGGTTTCAGTTTTTATGCTGTGTGCTGGAGTTACGCTTGTACAGTG; GAAACCAGCCCAAGCTACAAAAGTGGTGGTGGAACAAAATCCATTATTAGGGTTTGGCGC; TATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGGAGTATATTTTGAAAAAGTTTTAAA; GAGTTCAGATACTTCTCTTTGGGTGAGAAACATTCAAATGTATCTATCANGGATTATTGT; GACATTAGCTGGCGTCTACTTGTCAGATGGAGCTGANATTAAAGAAAAAGGATTTTTCTA; TGGTTACACATATTATGTCTGGGTTTGTCATCTTTCTTGCAAGTGTTGGTGGCCTCTACA; CTTCTGTTGTGGTTAAGTACACAGACNACATCATGAAAGGCTTTTCTGCAGCAGCGGCCA; TTGGTCCTTTNCCACCATTGCNTTCAGTATGCTGTTTGGGATACAGANTACACTCACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006416 unedited; CCGCGGCCGCAATCNAAAGTCGAGTTTTTTTTTTTTTTTTTTCTTTCTTACAAATAAAGT; TTATTGCTGAATTTCCCCATTAACATTATAGAAAAACACTGGAAATTTCACAAATTATTG; GAGAGCCCAACAGTTAAACATACTTTATTTAAAAAAGTACAAAAGTGACATTAGAAATTT; TTTTGAAGAAATGTGTATCATCTAACAGAAAAGAAATATGAACCAGATAATGAATGGCAC; AAATATAGCACTAAAGGGGTACTCACTAAAGGGGTACTCAGTCACCACCCAGAAATTGTC; CGAGTTATGAAATAGATTCATTTTGAGAAGTTACACAGTTTGTTTATGAACTAGCCTGTC; TTGTTTCTGCCTCTTGTAACAAAAGAGCTAGGTCTTTATGCTGCTAGGACAAAATACTGT; ACATGAATTGGAGAATAAGGAGGGGCCATCCTTCTCCCCGGTACCGGAACACGAGACCAG; TTTCGTCAGAACTGGTTTTGGCCCTTTAACCCTTAAGTATTGTCCCGAACCTTGGTCCTG; GACATTCGGGTGCTTACGATGCAATTGTATCCCCCCCCCCTTTTTGCTGCTCCGGCCCGC; TATTTGTAGCGGTTTCGCGACATTCCATGTTCTTTGTCGGGTGACCGCGCCCGTAACCCA; TATCTATTTCCCTTTCCCATATTCCGAATTCCCCCCCCCTTATCTCCCTCCTTTCTTCAA; TCCGCGACCCGCTCCCTTTTTCCCCTTCTCGCCCCCCCGGTGGTCCCCCGCCCCCTGCCC; TACCCGTGCCCTCTCTTACACTCTTTTCTCTTTCCCTTATTCCCCCCTTGTGCTTCTCCT; GCGCGTCGTTCATTCCTCCCCCCCCTCTTTCCCCCCTCCCCCCCATTCCCCCCCTTCGCT; CTGCGTTATCTCCTCCCCTTGCCCCCCGTTTGCTTCGTACCAAACGATGCACTATGTTTT; CCCTCCGCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006416
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006416.2, NP_006407.1
• RefSeq Size: 1883 bp
• RefSeq ORF: 1014 bp
• Locus ID: 10559
• UniProt ID: P78382
• Cytogenetics: 6q15
• Domains: Nuc_sug_transp
• Protein Families: Transmembrane
• Summary: The protein encoded by this gene is found in the membrane of the Golgi apparatus, where it transports nucleotide sugars into the Golgi. One such nucleotide sugar is CMP-sialic acid, which is imported into the Golgi by the encoded protein and subsequently glycosylated. Defects in this gene are a cause of congenital disorder of glycosylation type 2F (CDG2F). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).