PRPF8 (NM_006445) Human Untagged Clone
SKU
SC116070
PRPF8 (untagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8)
$2,011.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PRPF8 |
| Synonyms | HPRP8; PRP8; PRPC8; RP13; SNRNP220 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_006445 |
| Insert Size | 7140 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006445.2, NP_006436.2 |
| RefSeq Size | 7311 bp |
| RefSeq ORF | 7311 bp |
| Locus ID | 10594 |
| UniProt ID | Q6P2Q9 |
| Cytogenetics | 17p13.3 |
| Domains | JAB_MPN |
| Protein Families | Druggable Genome |
| Protein Pathways | Spliceosome |
| Summary | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006445" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209472 | PRPF8 (Myc-DDK-tagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) | 10 ug |
$1,927.00
|
|
| RC209472L1 | Lenti ORF clone of Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8), Myc-DDK-tagged | 10 ug |
$2,227.00
|
|
| RG209472 | PRPF8 (tGFP-tagged) - Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) | 10 ug |
$2,127.00
|
|
Citations
Spliceosomic dysregulation in pancreatic cancer uncovers splicing factors PRPF8 and RBMX as novel candidate actionable targets
Alors-Pérez, Blázquez-Encinas, Moreno-Montilla et al
Mol Oncol (2024)
Alors-Pérez, Blázquez-Encinas, Moreno-Montilla et al
Mol Oncol (2024)
Cited by 8
PRP4 Kinase Domain Loss Nullifies Drug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells
Ahmed, Islam, Sonn et al
Mol. Cells (2020) 43 (7), 662-670 DOI: 10.14348/molcells.2020.2263
Ahmed, Islam, Sonn et al
Mol. Cells (2020) 43 (7), 662-670 DOI: 10.14348/molcells.2020.2263
Cited by 8
AI Summary: In this study, the PRP8 clone from OriGene was used to assess the role of PRP8 in drug resistance and epithelial-mesenchymal transition in colorectal carcinoma cells, demonstrating that PRP8 did not reverse curcumin-induced apoptosis unlike PRP4.
Snippet: were obtained from Gibco (USA). PRP4 cDNA open reading frame clone HG10835-ACG was purchased from Sino Biological (USA), and a PRP8 clone was obtained from Origene (USA). Antibodies against caspase-3, cleaved
Decursin negatively regulates LPS-induced upregulation of the TLR4 and JNK signaling stimulated by the expression of PRP4 in vitro
Ahmed, Islam, Lee
Anim Cells Syst (Seoul) (2020) 24 (1), 44-52 DOI: 10.1080/19768354.2020.1726811
Ahmed, Islam, Lee
Anim Cells Syst (Seoul) (2020) 24 (1), 44-52 DOI: 10.1080/19768354.2020.1726811
Cited by 12
AI Summary: In this study, OriGene’s PRP4B Myc-DDK tagged expression plasmid (Cat# RC204726) was used to overexpress PRP4 in cells, enabling the investigation of its role in LPS-induced inflammatory responses and the regulatory effects of decursin.
PRPF8 is important for BRCA1-mediated homologous recombination
Onyango, Lee, Stark
Oncotarget (2017) 8 (55), 93319-93337 DOI: 10.18632/oncotarget.21555
Onyango, Lee, Stark
Oncotarget (2017) 8 (55), 93319-93337 DOI: 10.18632/oncotarget.21555
Cited by 22
AI Summary: In this study, OriGene’s PRPF8 shRNA (cat# TL311271) and PRPF8 cDNA (cat# RC219668) products were used to deplete and re-express PRPF8, respectively, enabling the authors to demonstrate the essential role of PRPF8 in BRCA1-mediated homologous recombination.
Splicing factor Prp8 interacts with NES(AR) and regulates androgen receptor in prostate cancer cells
Wang, Nguyen, Masoodi et al
Mol. Endocrinol. (2015)
Wang, Nguyen, Masoodi et al
Mol. Endocrinol. (2015)
Cited by 26
AI Summary: OriGene’s human Prp8 cDNA plasmid (Cat# sc116070) was used as a PCR template to construct Prp8 expression vectors, enabling the study of Prp8’s role and interaction with the androgen receptor in prostate cancer cells.
The core spliceosome as target and effector of non-canonical ATM signalling
Tresini, Warmerdam, Kolovos et al
Nature (2015)
Tresini, Warmerdam, Kolovos et al
Nature (2015)
Cited by 255
Showing 1-6 of 9 papers.
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