SLBP (NM_006527) Human Untagged Clone

SKU SC116017

SLBP (untagged)-Human stem-loop binding protein (SLBP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLBP
• Synonyms: HBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116017 sequence for NM_006527 edited (data generated by NextGen Sequencing); ATGGCCTGCCGCCCGCGAAGCCCGCCGAGGCATCAGAGCCGCTGCGACGGTGACGCCAGC; CCGCCGTCCCCCGCGCGATGGAGCCTGGGACGGAAGCGCAGAGCCGACGGCAGGCGCTGG; AGGCCCGAAGACGCCGAGGAGGCAGAGCACCGCGGCGCCGAGCGCAGACCCGAGAGCTTT; ACCACTCCTGAAGGCCCTAAACCCCGTTCCAGATGCTCTGACTGGGCAAGTGCAGTTGAA; GAAGATGAAATGAGGACCAGAGTTAACAAAGAAATGGCAAGATATAAAAGGAAACTCCTC; ATCAATGACTTTGGAAGAGAGAGAAAATCATCATCAGGAAGTTCTGATTCAAAGGAGTCT; ATGTCTACTGTGCCGGCTGACTTTGAGACAGATGAAAGTGTCCTAATGAGGAGACAGAAG; CAGATCAACTATGGGAAGAACACAATTGCCTATGATCGTTATATTAAAGAAGTCCCAAGA; CACCTTCGACAACCTGGCATTCATCCCAAGACCCCTAATAAATTTAAGAAGTATAGTCGA; CGTTCATGGGACCAGCAAATCAAACTCTGGAAGGTGGCTCTGCATTTTTGGGATCCTCCA; GCGGAAGAAGGATGTGATTTGCAAGAAATACACCCTGTAGACCTTGAATCTGCAGAAAGC; AGCTCCGAGCCCCAGACCAGCTCTCAGGATGACTTTGATGTGTACTCTGGCACACCCACC; AAGGTGAGACACATGGACAGTCAAGTGGAGGATGAGTTTGATTTGGAAGCTTGTTTAACT; GAACCCTTGAGAGACTTCTCAGCCATGAGCTAA; ; Clone variation with respect to NM_006527.2; 453 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006527 unedited; TCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGGCGGGACGCGG; GTTTCTGCCTCAGGCCCTGCCCTGCTCTACTCTGCGCTCTCTGCCCGCGCCGCCGCCGCC; TCAGCCTCGGCCCTGCGCTGCGCGCCCGGCCCGTGCTGCCATGGCCTGCCGCCCGCGAAG; CCCGCCGAGGCATCAGAGCCGCTGCGACGGTGACGCCAGCCCGCCGTCCCCCGCGCGATG; GAGCCTGGGACGGAAGCGCAGAGCCGACGGCAGGCGCTGGAGGCCCGAAGACGCCGAGGA; GGCAGAGCACCGCGGCGCCGAGCGCAGACCCGAGAGCTTTACCACTCCTGAAGGCCCTAA; ACCCCGTTCCAGATGCTCTGACTGGGCAAGTGCAGTTGAAGAAGATGAAATGAGGACCAG; AGTTAACAAAGAAATGGCAAGATATAAAAGGAAACTCCTCATCAATGACTTTGGAAGAGA; GAGAAAATCATCATCAGGAAGTTCTGATTCAAAGGAGTCTATGTCTACTGTGCCGGCTGA; CTTTGAGACAGATGAAAGTGTCCTAATGAGGAGACAGAAGCAGATCAACTATGGGAAGAA; CACAATTGCCTATGATCGTTATATTAAAGAAGTCCCAAGACACCTTCGACAACCTGGCAT; TCATCCCAAGACCCCTAATAAATTTAAGAAGTATAGTCGACGTTCATGGGACCAGCANAT; CANACTCTGGAAGGTGGCTCTGCATTTTTGGGATCCTCCAGCGGAAGAGGATGTGATTTG; CAAGAATACACCCTGTAGACCTTGATCTGCAGAAGCAGCTCCGAGCCCCAGACAGCTCTC; AGGAGACTTTGATGTGTACTCTGGCACACCCACCAGTTGAGACCATGNACAGTCAGTGGN; AGGATGAGTTGATTTGGAAGCTGGTTTACTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006527 unedited; ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTGAGGTTTTTACCACCCAGGGGAGTTTTAACTTTATTGGGGGGG; GGGGGATCTAAAACACTCTGAAAAAACCAATCTCGAGTTTTTTTTTTTTTTTTTTGAGGG; GTCTTTCCTTTTTATTTTTGCCATTTCCCCTTCCAAAGCCATTAAAACCTTACCGTCTTA; ACAAAAAATTTACCATTGAATATTTGAACCAAAAATTTGGACCCCTTTTCATTTACCGCA; ATTTTACCTGGGAAAATAACCGGTTGGTTTAAAAAGGTCTTTTTAAAACGCTCCCCTCTG; GTGCCCTAACTTTTGGGGGATTCTAAAAAAGCTAACCTGGGCTTTTTACCAGGCCCCAAT; TAAAACCCTTTAACTTACCTAAAACCCATTAGTTTAACCGCTCAATTTAACAATGGCTGA; CTTCAAAAAGCTGGTTTGACCTATTGGTAAAAGGGCCAAAATTAAGTTTCCCTAAAAATA; TTAAAGGGGGCTGAAAATTCCTTGATGCCCATAAAAGGAGCCGACCCTCAAATGGGCCCC; CGGGTTACATGGGTAAATTCAAAACAGGGCACCTGAACAGCAATAACCTGAAAAAGCCCG; TGGAAAACCCCTTTTGTAAACCCGTCACCTTACCCTGGAAGGCAACCCGGGTTACCCCAT; TAAGCCCCCTTGAAAAAACCTCAAGGACCTTTTTGGGACTAACCCCTTTTTCCCTTTAAA; AACTGCACAAATTACCCCCCTTACCTAAAAATTAAGTTTCTCTAAAAAAGGACGGCAAAA; TAATCTACCTGGCCCTAAGGGCCCGTTACAAAAACACACCCGGCCCAGGGCCACACCTGC; TTTGGGCCGGCCCGCCTCCCCCAAG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006527
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006527.2, NP_006518.1
• RefSeq Size: 1743 bp
• RefSeq ORF: 813 bp
• Locus ID: 7884
• UniProt ID: Q14493
• Cytogenetics: 4p16.3
• Summary: This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).