MEKK2 (MAP3K2) (NM_006609) Human Untagged Clone

SKU SC115980

MAP3K2 (untagged)-Human mitogen-activated protein kinase kinase kinase 2 (MAP3K2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MEKK2
• Synonyms: MEKK2; MEKK2B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006609 edited; ATGGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCC; AGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAA; CAGAATGATGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCC; AGACCAGTTAAACTGGAAGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAGTCTATG; GATCTACATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTGGAC; AAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGATATTACTTGTA; ATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGAT; AATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGA; GATAGAAGTTCTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAAT; GGGTCATTCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATG; CTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGTTGTCCATCACTT; GATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAATGCCTAGGGCTCAGAGCTAC; CCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAAATTTGGAAAA; GGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGT; CGTAAAACTTTTCCAAGAGCTAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGT; TTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAG; TATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTA; ATGGACATCAGCCCACCCAGCCGTTCACCTCGAGCTCCGACCAACTGGAGATTGGGCAAA; CTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGA; GAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAAGTA; AATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAG; TATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATG; CCAGGGGGTTCAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACT; AGGAAATACACCCGTCAGATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTC; CATAGAGATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTAGGA; GATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGACAGGAATGAAGTCT; GTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGGCTATGGAAGA; AAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCT; TGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCA; AAGCTGCCACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAG; GCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGTGCATTATCACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006609 unedited; CGCGTCAGATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGGCCTGA; CGAGGGTGCCGCGGCCGCTGGGTGCAGCGTTCGGGGGTTGCACGGCCCGCGGCGGCGACG; AAAACAACGACCAGGACGCTCGGCGCCCCTGTCCGCAGAGTCGCCACTCCCGCCTCTTCT; CCGGTCTCTAGCGACGCGGGCCCGCGGCTTCCTCCTTTCCTGCCCAGTTCCCGCGGGCCT; CGGGGTTGCAGCGGCTAGAAGCGCCGAGGAGCGCCAGGAGGACCCGGCCGGCTGCGAGGA; TTCACGCGCCCGCCACCCCGCCCGGCCCGGGCTTCATGTGAAAGAGGGACGGGCCTGGCC; CCAAGGCCCGCGCCCGACTCCTCGCGCGCCCCTCCGGTCCCCGGGGGCCCTGCGGCAGCC; CCCGACCTGGGGCCGCGGTGACAGGCCGCGCTGGGGCGGAGGGGCGGGCTCGGCGGGGGC; GGCCCCTCCTTGGGCCTGCGGCTCTCTACGTCCCTGCGGAGCAGCCGGCCATAAAGAATG; CTGATGGGAGAACCATTTTCCTAATTTTCAAATTGTTGAGCTGTTTGCCATAATGGATGA; TCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACC; AGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGA; TGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGT; TAAACTGGAAGATCTGAGATCTAAAGCTAANNATGCCTTTGGACAGTCTATGGATCTACA; TTATACCAATAACGAGTTGGGTAATCCATTAACTACTCAAGATGACTGGNACAAAGCTGT; GGAACTGCTGGATCGTAGTATTCATATGAAGAGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006609 unedited; TGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGCCCAAATCATGA; ATTTTAAGGAAAGAATATATAGAGATGGCTGAGTTCAAAAGGCTCAGTGTTCAAATTCAG; TTTGTTCCTAAAATGAAGAGTTACCTATGTGGGTGCAATATGCAGCTGGTAAAGTGATTG; CTATTTGCTGTTTGTTGAGATTATTCACCCTTGACTTAAAGCAGCAGTATCTGATCTTGT; AAAATCCTCAATTTGCATTACATCACTTTCTCTTTGCGACTTCCTTTTCTTTCTTGCATT; TACTGCTTTGTAAATAGCTGTTTTCAGTTTATAACTGGGACTGATCTTTACATCAGGGTT; TCTCAGCCTCAGCACTTCTGACATTTTGGGAGGGGTAATTCTTTGAGGCTGCTTTCCTTG; TGTATTATAATCTATTTAGCAACATCCCTGGCCTCTACCCAATTCATGCTACTAGTATCC; CTCCAATTGTGACAACCGGAAATGTCTCTAAGAATTGCCAAATGCCTAGTGAAATCATCC; TCGCTCCACTTTTGGCAACCACTGTTTCACATGATACCTGTTTTTTTGAGGAGCTTTAGT; ATTCTGTGATCCTAAGAACAAGGGTTTCATCTCCTGACATAACACATAAACATTACTAAT; TGAACTCTTCGCTCCTAAGGGATGTTACCTATGGGGAATCAGGAGCTGGAAATAGAAGAT; GGTATACATGATTCTGATTATTTCTNCATTCCTTTAATTCTGGGACGTNCCCCTCAAGTA; AAACAAAACAAAACTGTTTAAAATACACTAAACACCTAATTTTTCTTCATTATTTCTATT; GACTGGTAATGAGGNCTCCNCTACTGCTCAACATTTGATAAGATGATGNCCTTTTGTCTC; TATTTCTATTTGCTCACACTTGACTTTAAATAAGGCTCTNCGCGNGTGGTGGTGAGGGGG; GGTTTTTAGCAGAAGCTTCCCTCAGCTTACGATCCAGTACGGGATCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006609
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006609.2, NP_006600.2
• RefSeq Size: 3336 bp
• RefSeq ORF: 1863 bp
• Locus ID: 10746
• UniProt ID: Q9Y2U5
• Cytogenetics: 2q14.3
• Domains: PB1, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway
• Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]