ELF2 (NM_006874) Human Untagged Clone

SKU SC115824

ELF2 (untagged)-Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ELF2
• Synonyms: b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006874 edited; GAATTCGGCACGAGGCCGGCCGCGGGTTCCGCCTCCTCCCCTGCCGCCGCTGCTCACGGT; GTAAGTCAATGTGAAGCAGCAGCTCCAGCCCCGGGATAAACATGGCGACGTCTCTGCATG; AGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAGTTCACAGCA; GTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAAT; CTCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTAT; CAACTCCAGAATTCATCCATGCTGCTATGAGGCCAGATGTCATTACAGAAACTGTAGTGG; AGGTGTCAACTGAAGAGTCTGAACCCATGGATACCTCTCCTATTCCAACATCACCAGATA; GCCATGAACCAATGAAAAAGAAAAAAGTTGGCCGTAAACCAAAGACCCAGCAATCACCAA; TTTCCAATGGGTCTCCTGAGTTAGGTATAAAGAAGAAACCAAGAGAAGGAAAAGGAAACA; CAACCTATTTGTGGGAGTTTCTTTTAGATCTACTTCAAGATAAAAATACTTGTCCCAGGT; ATATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCT; CTAAGCTTTGGGGAAAGCATAAGAACAAACCAGACATGAACTATGAAACCATGGGACGAG; CTTTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTAT; ATCAGTTCAAGGATATGCCGAAAAACATAGTGGTCATAGATGATGACAAAAGTGAAACCT; GTAATGAAGATTTAGCAGGAACTACTGATGAAAAATCATTAGAACGAGTGTCACTGTCTG; CAGAAAGTCTCCTGAAAGCAGCATCCTCTGTTCGCAGTGGAAAAAATTCATCCCCTATAA; ACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGTTGTGAATATCACTTCCCCTGGGCACG; ATGCTTCATCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCAACAGCAGCTCCAAGGA; CAGTTCGTGTGGCAATGCAGGTACCTGTTGTAATGACATCATTGGGTCAGAAAATTTCAA; CTGTGGCAGTTCAGTCAGTTAATGCAGGTGCACCATTAATAACCAGCACTAGTCCAACAA; CAGCGACCTCTCCAAAGGTAGTCATTCAGACAATCCCTACTGTGATGCCAGCTTCTACTG; AAAATGGAGACAAAATCACCATGCAGCCTGCCAAAATTATTACCATCCCAGCTACACAGC; TTGCACAGTGTCAACTGCAGACAAAGTCAAATCTGACTGGATCAGGAAGCATTAACATTG; TTGGAACCCCATTGGCTGTGAGAGCACTTACCCCTGTTTCAATAGCCCATGGTACACCTG; TAATGAGACTATCAATGCCTACTCAGCAGGCATCTGGCCAGACTCCTCCTCGAGTTATCA; GTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAGAACATG; ATGTGAAAACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGAAATAAGACAGTGACCC; ACGTAGTGGTTGTCAGTGCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAAACAGAAG; GACTAGTGACATGTGAGAAATAAAATAGCAGCTCCACCATGGACTTCAGGCTGTTAGTGG; CAGTACTGACATAAACATTTGCAAGGGAAGTCATCAAGAAAAGTCAAAGAAGACTTTAAA; ACATTTTTAATGCATATACAAAAACAATCAGACTTACTGGAAATAAATTACCTATCCCAT; GTTTCAGTGGGAAATGAACTACATATTGAGATGCTGACAGAAAACTGCCTCTTACAGTAG; GAAACAACTGAACCCATCAATAAGAAAAAGGATCGAAAGGGACCAAGCAGCTCACTACGA; TATCAAGTTACACTAAGACTTGGAACACTAACATTCTGTAAGAGGTTATATAGTTTTCAG; TGGGAGGGGTTGGGATGGGTAATCTCATTGTTACATATAGCAATTTTTGATGCATTTTAT; ATGCATACCAGCAATTATTACTGTGTTCGCACAGTTCTCACTTAACTGGTGCTATGTGAA; GACTCTGCTAATATAGGTATTTTAGAATGTGAATTGAAGAATGGATCCCAAAAACTTCAG; AAAGAGGATAGCAAAAAAAGATCTAGTGCGATTTTATATATATATATATATATATATACA; TACATATATATATATCATATAGCTTAAGCTGATTTAAAACAAAGGCCTTAGACTAATTTT; CGATTTTCTTTCTTGAAATAAGCTAATGGCTTGTTTGTGTAAAGCTTTTTTATTAAAAGA; AAAATTTTAAAAATCTAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006874 unedited; GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGGCCGCGG; GTTCCGCCTCCTCCCCTGCCGCCGCTGCTCACGGTGTAAGTCAATGTGAAGCAGCAGCTC; CAGCCCCGGGATAAACATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCT; GCTCATCCGGGCCGTGGAAGCATCAGTTCACAGCAGTAATGCACACTGTACAGATAAGAC; AATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATCTCCTACCTGCTTGAGGGATTCAAG; AAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTATCAACTCCAGAATTCATCCATGCTGC; TATGAGGCCAGATGTCATTACAGAAACTGTAGTGGAGGTGTCAACTGAAGAGTCTGAACC; CATGGATACCTCTCCTATTCCAACATCACCAGATAGCCATGAACCAATGAAAAAGAAAAA; AGTTGGCCGTAAACCAAAGACCCAGCAATCACCAATTTCCAATGGGTCTCCTGAGTTAGG; TATAAAGAAGAAACCAAGAGAAGGAAAAGGAAACACAACCTATTTGTGGGAGTTTCTTTT; AGATCTACTTCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAA; AGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCTCTAAGCTCTGGGGAAAGCATAAGAA; CCAACCAGACATGAACTATGAAACCATGGGACGAGCTTTGAGATACTACTACCAAAGGGG; AAATTCTGCCAAGGTTGAACGACAGAGGCTTGTATATCAGTTCCAGGATATGCCCGAAAA; CATAGTGGTCATAGATGATGACACAGTGAAACCCTGTATGAAGATTTACCAGGACCTACT; GATGGAAAATCATTAGAACGAGTGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006874 unedited; ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTAAAATTTTT; CTTTTAATAAAAAAGCTTTACACAAACAAGCCATTAGCTTATTTCAAGAAAGAAAATCGA; AAATTAGTCTAAGGCCTTTGTTTTAAATCAGCTTAAGCTATATGATATATATATATGTAT; GTATATATATATATATATATATATAAAATCGCACTAGATCTTTTTTTGCTATCCTCTTTC; TGAAGTTTTTGGGATCCATTCTTCAATTCACATTCTAAAATACCTATATTAGCAGAGTCT; TCACATAGCACCAGTTAAGTGAGAACTGTGCGAACACAGTAATAATTGCTGGTATGCATA; TAAAATGCATCAAAAATTGCTATATGTAACAATGAGATTACCCATCCCAACCCCTCCCAC; TGAAAACTATATAACCTCTTACAGAATGTTAGTGTTCCAAGTCTTAGTGTAACTTGATAT; CGTAGTGAGCTGCTTGGTCCCTTTCGATCCTTTTTCTTATTGATGGGTTCAGTTGTTTCC; TACTGTAAGAGGCAGTTTTCTGTCAGCATCTCAATATGTAGTTCATTTCCCACTGAAACA; TGGGATAGGTAATTTATTTCCAGTAAGTCTGATTGTTTTTGTATATGCATTAAAAATGTT; TTAAAGTCTTCTTTGACTTTTCTTGATGACTTCCCTTNGCAATGTTTATGTCAGTACTGC; CACTAACAGCCCTGAAGTCATGGTGGAGCTGCTATNTTATTTCTCACATGTCACTAGCCC; CTTCTGGTTTCATAGNTACAGGAAGGGCCAATAGCTGGAGGCGCACTGACAACCACCTAC; GGGGGCCACTGGCCTTATTCCCTCTGGCTGGGTTTTCTNCTACAAGCCTGCAAGTTTCAC; CTCATGGTTTTGGTTTTTTGCCACTGCTTCGATTAACCCTCGCCCCCTTAGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006874
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006874.2, NP_006865.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 1602 bp
• Locus ID: 1998
• UniProt ID: Q15723
• Cytogenetics: 4q31.1
• Domains: ETS
• Protein Families: Druggable Genome, Transcription Factors
• Summary: Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) has an alternate exon in place of the first three exons and uses an alternate in-frame splice junction at the 5' end of an internal exon compared to variant 1. Translation starts in the alternate exon, so the resulting isoform (2) has a shorter and distinct N-terminus and contains an alternate internal segment compared to isoform 1.