RAB35 (NM_006861) Human Untagged Clone

SKU SC115816

RAB35 (untagged)-Human RAB35, member RAS oncogene family (RAB35), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAB35
• Synonyms: H-ray; RAB1C; RAY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115816 sequence for NM_006861 edited (data generated by NextGen Sequencing); ATGGCCCGGGACTACGACCACCTCTTCAAGCTGCTCATCATCGGCGACAGCGGTGTGGGC; AAGAGCAGTTTACTGTTGCGTTTTGCAGACAACACTTTCTCAGGCAGCTACATCACCACG; ATCGGAGTGGATTTCAAGATCCGGACCGTGGAGATCAACGGGGAGAAGGTGAAGCTGCAG; ATCTGGGACACAGCGGGGCAGGAGCGCTTCCGCACCATCACCTCCACGTATTATCGGGGG; ACCCACGGGGTCATTGTGGTTTACGACGTCACCAGTGCCGAGTCCTTTGTCAACGTCAAG; CGGTGGCTTCACGAAATCAACCAGAACTGTGATGATGTGTGCCGAATATTAGTGGGTAAT; AAGAATGACGACCCTGAGCGGAAGGTGGTGGAGACGGAAGATGCCTACAAATTCGCCGGG; CAGATGGGCATCCAGTTGTTCGAGACCAGCGCCAAGGAGAATGTCAACGTGGAAGAGATG; TTCAACTGCATCACGGAGCTGGTCCTCCGAGCAAAGAAAGACAACCTGGCAAAACAGCAG; CAGCAACAACAGAACGATGTGGTGAAGCTCACGAAGAACAGTAAACGAAAGAAACGCTGC; TGCTAA; ; Clone variation with respect to NM_006861.6;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006861 unedited; AGCATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCCGCCGCTGCCGG; AGCAGCCCGAAGGAGCTGCGGATCGCGAGGCCAGTACCGACCCCGCCCGCCCGCGCGCAC; CGCCCCCGCCCGCCATGGCCCGGGACTACGACCACCTCTTCAAGCTGCTCATCATCGGCG; ACAGCGGTGTGGGCAAGAGCAGTTTACTGTTGCGTTTTGCAGACAACACTTTCTCAGGCA; GCTACATCACCACGATCGGAGTGGATTTCAAGATCCGGACCGTGGAGATCAACGGGGAGA; AGGTGAAGCTGCAGATCTGGGACACAGCGGGGCAGGAGCGCTTCCGCACCATCACCTCCA; CGTATTATCGGGGGACCCACGGGGTCATTGTGGTTTACGACGTCACCAGTGCCGAGTCCT; TTGTCAACGTCAAGCGGTGGCTTCACGAAATCAACCAGAACTGTGATGATGTGTGCCGAA; TATTAGTGGGTAATAAGAATGACGACCCTGAGCGGAAGGTGGTGGAGACGGAAGATGCCT; ACAAATTCGCCGGGCAGATGGGCATCCAGTTGTTCGAGACCAGCGCCAAGGAGAATGTCA; ACGTGGAAGAGATGTTCAACTGCATCACGGAGCTGGTCCTCCGAGCAAAGAAAGACAACC; TGGNCAAACAGCAGCAGCAACAACAGAACGATGTGGTGAAGCTCACGAAGAACAGTAAAC; GAAAGAAACGCTGCTGCTAATGGCACCCAGTCCACTGCAGAGACTGCACTGCGGTCCCTC; CCCCAGCCCGAGGCCCACGGAGGTTCCTCGGGGGACAGTCTCAGTTTCGTGCCGTTATTT; AAAGAAATCTCTNCCATGTTTTGTATCGGGAGGTGCCATCGGACTTCCTCCCCCGCCTNC; TCGAGTGCCAGAAGTGTTGGACAGCCCGCCTTCCTATGGGCCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006861 unedited; TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACTTGATTTG; GCTTCATTTTCTTGATCTGTTAAAATAATCCTCCCATAGCCCCCCTGCCAGCCCCATCTT; TGCACGAACCTACCCCGACCTTTCTGTTGGAACTGAAACCTGTTGGTGTAAATGAAAAGC; CATGGCTGCCCTGGGTTTGGAGCTCAAAGGCATCTAAAAGGCAGGACAAGAAATCTGTTG; GCCAAAGGGCAAGACCTGCCACCTCTGTGGAACTGCAGGGCCTGCCTTGAAACCAGGTTC; CCCAGCTCCCAAAATGGCTGTGGGGACGCAACAACGGGGAGGGAAGGGAGCTGGCACAGG; CCCTGGAGAAGGGGCAAGACCCTGTGCAGCGGGGACAGAGGCTGACAACCTGTCGGAGAG; AATGAGCAACGCGGAGCCCACTCCACTGGCACGGGCTGGCCGCAAACGCAGCTAGAACGT; GCCGCTGTCTCCTCAGGACGCTGCTTGCAGTAAAATGGGCTGGGGAGGGGCGCGGGGAGG; GTCTGTTGGAGCAGGCTGGCATCAAGAAGGCAAAAGCCAAAGCAGGACGTGGTGTGCGGC; CGGGTAAAGCAATATACACTATGTACAGACTCTGCGAATCAGTCCGCTCGGCGGGCAGCG; TCCTCGCCAGGTCCAGGCGCCTTTGGCCGGGGAAGGAGGGGGGCACCAGTAGGGGAAGGG; GCGGGCTGGGTCCAACACCCTTCTTGGCACTCNAGGAGGGCGGGGNAAGGAAATGCCGAT; GGNCACCTCCCGAACAAAAACTGGAAAGATTCTTTAAATACGCACGAACTGAAATGTCCC; CGAGAACCTNCGTGGCCTGGGCTGGGGGAGGACCNNATGCANCCTTGCATGGACTGGTGC; ATTACANCACGTTTCTTCTTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006861
• Insert Size: 1580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006861.4, NP_006852.1
• RefSeq Size: 2931 bp
• RefSeq ORF: 606 bp
• Locus ID: 11021
• UniProt ID: Q15286
• Cytogenetics: 12q24.23
• Protein Families: Druggable Genome
• Summary: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longer isoform (1).