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SDF2 (NM_006923) Human Untagged Clone

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SKU
SC115785
SDF2 (untagged)-Human stromal cell-derived factor 2 (SDF2)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SDF2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115785 sequence for NM_006923 edited (data generated by NextGen Sequencing)
ATGGCTGTAGTACCTCTGCTGTTGTTGGGGGGTTTGTGGAGCGCTGTGGGAGCGTCCAGC
CTGGGTGTCGTTACTTGCGGCTCCGTGGTGAAGCTACTCAATACGCGCCACAACGTCCGA
CTGCACTCACACGACGTGCGCTATGGGTCAGGTAGTGGGCAGCAGTCAGTGACAGGTGTA
ACCTCTGTGGATGACAGCAACAGTTACTGGAGGATACGGGGGAAGAGTGCCACAGTGTGT
GAGAGGGGAACCCCCATCAAGTGTGGCCAGCCCATCCGGCTGACACATGTCAACACTGGC
CGAAACCTCCATAGTCACCACTTCACTTCACCTCTTTCTGGAAACCAGGAAGTGAGTGCT
TTTGGTGAGGAAGGTGAAGGTGATTATCTGGATGACTGGACAGTGCTCTGTAATGGACCC
TACTGGGTGAGAGATGGTGAGGTGCGGTTCAAACACTCTTCCACTGAGGTACTGCTGTCT
GTCACAGGAGAACAATATGGTCGACCTATCAGTGGGCAAAAAGAGGTGCATGGCATGGCC
CAGCCAAGTCAGAACAACTACTGGAAAGCCATGGAAGGCATCTTCATGAAGCCCAGTGAG
TTGTTGAAGGCAGAAGCCCACCATGCAGAGCTGTGA

Clone variation with respect to NM_006923.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006923 unedited
NAAAGTCGGAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCGGGAG
TGGGCGGGCCATTTCTTGTTCTCTCTCCCGCTCTCGGAAGCTTTCGTCTCGTGGGTGCGA
AAGGTAACCGAAGCGGCTCAGGAAGGCAGCTGTCACTGAGCCCCTGGAACAGAGCGAGAG
TATCGTAAGTAACCAGGCTCAGCCGGTTTCTCAGGCCGCTCTAGTCAAATAAACCATAAA
GATCAGACTCGGGCTTCTTCACTTCCTTCTCTCCGTGGTTTCGCCATTAGCTTCCGGTTC
CGGGGAGGGGCCGAGTTTTCTTCGAAGATTTGGGGCTCCGCGATACAGTTAGGATGGCTG
TAGTACCTCTGCTGTTGTTGGGGGGTTTGTGGAGCGCTGTGGGAGCGTCCAGCCTGGGTG
TCGTTACTTGCGGCTCCGTGGTGAAGCTACTCAATACGCGCCACAACGTCCGACTGCACT
CACACGACGTGCGCTATGGGTCAGGTAGTGGGCAGCAGTCAGTGACAGGTGTAACCTCTG
TGGATGACAGCAACAGTTACTGGAGGATACGGGGGAAGAGTGCCACAGTGTGTGAGAGGG
GAACCCCCATCAAGTGTGGCCAGCCCATCCGGCTGACACATGTCAACACTGGCCGAAACC
TCCATAGTCACCACTTCACTTCACCTCTTTCTGGAACCAGGAAGTGAGTGCTTTTGGGAA
GGAGGTTGAAGGTGATTATCTGGATGACTGGACAGTGCTCTGTAATGGACCCTACTGGGT
GAGAATGGTTGAAGGNCCGGTTCAAACACTCTTCCCCTGAAGTACTGCCTGNCCTGCCCA
GGAGAACATAATGGTCTACTTTCAACGGGCAATAAGAAGCTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006923 unedited
ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATAATAAAATTAT
TGTTTTTATCATTTTGGAGAGAAAAATAGGGGGAAAACACATACATTAACAGTCCATGAT
GCCAAGCTCCTGAAGAGTAAAAGTTTCCCAGCTAAGAGGGATGTTTCCCTCAGTTTGTTT
TATCAGTACTAATAAAAAGCATCTGCCCCTTTACCAGCAAGTCCTCTACTCAAAAAGAAC
TGACCCACGCAAGTCTGGGAGAGTGACTAGTTCAAATGTGCAGGGCTGAAGCTTCCAAAC
ACAGCCACTATTTTCTGTGGTATATCTTCATCTCAATGGTGACATGGCCACTGCCCAAGG
AACTTGTGGCAGGGATCCCAAGGTGAGGCAGCAACAGATGTCTGTGAACATTGTGCGTTA
ACAGTGGCTCAGAGCCTCTAGATTCACAGCTTTGCATGGTGGGCTTCTGCCTTCAACAAC
TCACTGGGCTTCATGAAGATGCCTTCCATGGCTTTCCAGTAATTGTTCTGACTTGGCTGG
GCCATGCCATGCACCTCTTTTTGCCCACTGATAGGTCGACCATATTGTTTTCCTGTGACA
GACAGCAGTTCCTTCATTGGAAAAGTGTTTGAACCCGAACCTAATCCTCTTTCCCCCAGT
ATGGTCCATTCCATAACCCTGTCCATTATTCAGATATAAACTTTACCTTTCTTACACAAA
GCCCTTACTTTTTGGTTTCCAAAAGTAGGGAATTGAACTTGTGACTTTGGGTGTTCGCGC
ACGTGTTCCTTGCGCAAGCGGATGGGCCCTCCCCATTTAGGGGGGCCCCCTCCTCCCATC
TGGCCACCTTTCCCCGCTCACTCTCAACCCTGTCGCTGCATACACAAAGTATACCCA
Restriction Sites NotI-NotI |
ACCN NM_006923
Insert Size 1420 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006923.2, NP_008854.2
RefSeq Size 1075 bp
RefSeq ORF 636 bp
Locus ID 6388
UniProt ID Q99470
Cytogenetics 17q11.2
Domains MIR
Protein Families Secreted Protein
Summary The protein encoded by this gene is believed to be a secretory protein. It has regions of similarity to hydrophilic segments of yeast mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Alternate splicing results in both coding and non-coding variants. A pseudogene of this gene is located on chromosome 15. provided by RefSeq, Dec 2011
Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_006923" in other vectors (4)
SKU Description Size Price
RC200939 SDF2 (Myc-DDK-tagged)-Human stromal cell-derived factor 2 (SDF2) 10 ug
$300.00
RC200939L3 Lenti ORF clone of Human stromal cell-derived factor 2 (SDF2), Myc-DDK-tagged 10 ug
$600.00
RC200939L4 Lenti ORF clone of Human stromal cell-derived factor 2 (SDF2), mGFP tagged 10 ug
$600.00
RG200939 SDF2 (tGFP-tagged) - Human stromal cell-derived factor 2 (SDF2) 10 ug
$489.00 $500.00

file-alt Citations

Stromal cell-derived factor 2 is critical for Hsp90-dependent eNOS activation
Siragusa, Fröhlich, Park et al
Sci Signal (2015) 8 (390), ra81 DOI: 10.1126/scisignal.aaa2819
Cited by 25
AI Summary: In this study, OriGene products including SDF2 cDNA (RC201307) and SDF2 shRNA (TG311370) were used to overexpress and knockdown SDF2, respectively, enabling the authors to demonstrate SDF2’s critical role in Hsp90-dependent eNOS activation in endothelial cells.
FigureFigure
Showing 1-1 of 1 papers.
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