BTN2A2 (NM_006995) Human Untagged Clone

SKU SC115770

BTN2A2 (untagged)-Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BTN2A2
• Synonyms: BT2.2; BTF2; BTN2.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC115770 representing NM_006995.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCGGCCGCGAATTCGGCACGAGGGAGTCCGCAACTCCAAACATCGCGATTAATAGGAGGCCTCTGGTCT; CTGCCTGCCCTGGGTGCTCATGGAACCAGCTGCTGCTCTGCACTTCTCCCTGCCAGCCTCCCTCCTCCT; CCTCCTGCTCCTCCTCCTTCTCAGCCTGTGTGCACTGGTCTCAGCCCAGTTTACTGTCGTGGGGCCAGC; TAATCCCATCCTGGCCATGGTGGGAGAAAACACTACATTACGCTGCCATCTGTCACCCGAGAAAAATGC; TGAGGACATGGAGGTGCGGTGGTTCCGGTCTCAGTTCTCCCCCGCAGTGTTTGTGTATAAGGGTGGGAG; AGAGAGAACAGAGGAGCAGATGGAGGAGTACCGGGGAAGAATCACCTTTGTGAGCAAAGACATCAACAG; GGGCAGCGTGGCCCTGGTCATACATAACGTCACAGCCCAGGAGAATGGGATCTACCGCTGTTACTTCCA; AGAAGGCAGGTCCTACGATGAGGCCATCCTACGCCTCGTGGTGGCAGGCCTTGGGTCTAAGCCCCTCAT; TGAAATCAAGGCCCAAGAGGATGGGAGCATCTGGCTGGAGTGCATATCTGGAGGGTGGTACCCAGAGCC; CCTCACAGTGTGGAGGGACCCCTACGGTGAGGTTGTGCCCGCCCTGAAGGAGGTTTCCATCGCTGATGC; TGACGGCCTCTTCATGGTCACCACAGCTGTGATCATCAGAGACAAGTATGTGAGGAATGTGTCCTGCTC; TGTCAACAACACCCTGCTCGGCCAGGAGAAGGAAACTGTCATTTTTATTCCAGAATCCTTTATGCCCAG; CGCATCTCCCTGGATGGTGGCCCTAGCTGTCATCCTGACCGCATCTCCCTGGATGGTGTCCATGACTGT; CATCCTGGCTGTTTTCATCATCTTCATGGCTGTCAGCATCTGTTGCATCAAGAAACTTCAAAGGGAAAA; AAAGATTCTGTCAGGGGAAAAGAAAGTTGAACAAGAGGAAAAAGAAATTGCACAGCAACTTCAAGAAGA; ATTGCGATGGAGAAGAACATTCTTACATGCTGCTGATGTGGTCCTGGATCCAGACACCGCTCATCCCGA; GCTCTTCCTGTCAGAGGACCGGAAAAGTGTGAGGCGGGGCCCCTACAGGCAGAGAGTGCCTGACAACCC; AGAGAGATTCGACAGTCAGCCTTGTGTCCTGGGATGGGAGAGCTTCGCCTCAGGGAAACATTACTGGGA; GGTGGAGGTGGAAAACGTGATGGTGTGGACTGTGGGGGTCTGCAGACACAGTGTTGAGAGGAAAGGGGA; GGTCCTGCTGATTCCTCAGAATGGCTTCTGGACCCTGGAGATGTTTGGAAACCAATACCGGGCCCTGTC; CTCCCCTGAGAGGATTCTCCCTTTGAAGGAGTCCCTTTGCCGGGTGGGCGTCTTCCTGGACTATGAAGC; TGGAGATGTCTCCTTCTACAACATGAGGGACAGATCGCACATCTACACATGTCCCCGTTCAGCCTTTAC; TGTGCCTGTGAGGCCCTTCTTCAGGTTAGGGTCTGATGACAGCCCCATCTTCATCTGCCCTGCACTCAC; AGGAGCCAGTGGGGTCATGGTGCCTGAAGAGGGCCTGAAACTTCACAGAGTGGGGACCCACCAGAGCCT; ATAGAATCAATTCCTTGGACTCACAGCCATGCAGATAAGCCCTGGCCATCTCAGCAGCCACCGCACAAC; CCCCCTAATGAAAGACACGCCCTCCTCCCCTCTGGTCACGTAAGAGAACATCTTCCAGCTGCCTTTTTC; ACACCCACTCCAGCCCTCTGCCCCAGTTTTCTCCTCCTCACTAGTCTGTGGCTTTAGTAGTTCCTTTGC; TTGTAATTATGGGATGGGATCCAGGCATAGGGAACTAGTTGTTTCATAGCTCCCAGTCAAAAAGAAAGT; GAGAGAAGCTGTTGGGCAGCGAACCTACTGTTTAAAATCAGGATAACCACATTAAGCCCAATATGCCAG; TTGGCACCAGATGCTGTGGACTTGGAATGAGGCCAACAGGGTTCACCAGGATGAGAGAGGAGAGAGGAA; TCCACAGGACCACCAGAAGGGAGAGGGAACCAGATATGCAAATCAGAGATAGAGGAAGTGGAACCAGAG; AGCTGGGAGGGACCAAGGTTGTAAGGATGGCTAAGTCCCACCATAAGAGCTAAAGGGTCCTGGGAGATG; ATGGCTCATTTCCACCCAACCCCAGGATTTCCACAGCACACACCCACAGGCCTGGACCTGGGATGAAGA; TGAATGAAGAACATGGACTCATGTGGATGTGGTTTGGCTCAGATGTCCCTGCAATAAACAAGGGGTCAG; TACTTAGTCCCTGAGTGTGGTTGAGGTTTGAGGTCCTGGTCGAGCAGGGCAGTACTGGACCAGGTCTAC; GTCAGCATTCAGGTTCAATGGGGACACCAGTGGCTTCAAACTTCCTGATCTAATTATGTTTTTAGACAC; TTAGAAGTTATTGAGGACTTTAAAGAGCTTTTGTTTATTTGGGTTAATATTTATGACATTTGACATTGA; AACAAAAATTTAAAATGTTATCTTTTAATTTATGTTAAAATAGCATTAATAAATCAGTTATAGGTTAAT; GTAGATAGGATGTTTTGTGAAAAAGCAATCTATTGTGTCCAAATAAAAAAACAAAAAGTGAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006995 unedited; TCACGAATATGGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGGAGTCCG; CAACTCCAAACATCGCGATTAATAGGAGGCCTCTGGTCTCTGCCTGCCCTGGGTGCTCAT; GGAACCAGCTGCTGCTCTGCACTTCTCCCTGCCAGCCTCCCTCCTCCTCCTCCTGCTCCT; CCTCCTTCTCAGCCTGTGTGCACTGGTCTCAGCCCAGTTTACTGTCGTGGGGCCAGCTAA; TCCCATCCTGGCCATGGTGGGAGAAAACACTACATTACGCTGCCATCTGTCACCCGAGAA; AAATGCTGAGGACATGGAGGTGCGGTGGTTCCGGTCTCAGTTCTCCCCCGCAGTGTTTGT; GTATAAGGGTGGGAGAGAGAGACTAGAGGAGCAGATGGAGGAGTACCGGGGAAGAATCAC; CTTTGTGAGCAAAGACATCAACAGGGGCAGCGTGGCCCTGGTCATACATAACGTCACAGC; CCAGGAGAATGGGATCTACCGCTGTTACTTCCAAGAAGGCAGGTCCTACGATGAGGCCAT; CCTACGCCTCGTGGTGGCAGGCCTTGGGTCTAAGCCCCTCATTGAAATCAAGGCCCAAGA; GGATGGGAGCATCTGGCTGGAGTGCATATCTGGAGGGTGGTACCCAGAGCCCCTCACAGT; GTGGAGGGACCCCTACGGTGAGGTTGTGCCCGCCCTGAAGGAGGTTTCCATCCGCTGATG; CTGACGGCCTCTTCATGGTCACCACAGCTGTGATCATCAGAGACAAGTATGTGAAGAATG; TGTCCTGCTCTGTCACAACACCCTGCTCGGCCAGGAGAAAGAAACTGTCATTTTATTCCA; GAATCCTTAATGCCAGCGCATCTCCCTGGATGGGGGCCCTAATTGCCATCTGACCGCACT; CCCTGGATGGGGCCATGACTGCCATCCTGGCTGTTTTAACATCCTCATGGCTGCCACCTC; TGGTGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006995 unedited; TGTTGTTGTGTTTGCTCTCTCACCCCCTTCCCCACACCACCCTGTGGTGGNNNTNTTTTA; TCTGGTCTACATGANTATGCTATTTTACACAAAACATCCTATCTACATTAACCTATAACT; GATTTATTAATGCTATTTTAACATAAATTAAAAGATAACATTTTAAATTTTTGTTTCAAT; GTCAAATGTCATAAATATTAACCCAAATAAACAAAAGCTCTTTAAAGTCCTCAATAACTT; CTAAGTGTCTAAAAACATAATTAGATCAGGAAGTTTGAAGCCACTGAGGTCCCCATTGAA; CCTGAATGCTGACGTAGACCTGGTCCAGTACTGCCCTGCTCGACCAGGACCTCAAACCTC; AACCACACTCAGGGACTAAGTACTGACCCCTTGTTTATTGCAGGGACATCTGAGCCAAAC; CACATCCACATGAGTCCATGTTCTTCATTCATCTTCATCCCAGGTCCAGGCCTGTGGGTG; TGTGCTGTGGAAATCCTGGGGTTGGGTTGGAAATGAGCCATCATCTCCCAGGACCCTTTA; GCTCTTATGGTGGGACTTAGCCATCCTTACAACCTTGGTCCCTCCCAGCTCTTTGGTTTC; ACTTCCTCTATCTCTGATCTGCATATCTGGTTCCCTCTCCCTTCTGGTGGTCCTGAGGAT; TCCTCTTTCCTCTCTCATCCTGGAGAACCCTGTTGGCCTTATTCCAAGTCCACAGAATCT; TGGTGCCCAACTGGCATATTGGGCCTTAATGTGGTCTATCCTGGATTTTAAACAATAGGT; TTCGATGCCCCACCAGCTTTCTCTCACTTCTCTTTTTGACTGGGAAACTATGAAACAACT; ATTTTCCCTATGCCTGGTATCCTTCCCATTATTTCCAGCAAAGGAACTACTTAAGCCCCA; TAATATTTGAGGAGGATAAAAACTCGGCTCACATGCTCTGTATTGGTTGCTTAAAAATGT; ACTCTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006995
• Insert Size: 2746 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006995.3
• RefSeq Size: 3577 bp
• RefSeq ORF: 2746 bp
• Locus ID: 10385
• UniProt ID: Q8WVV5
• Cytogenetics: 6p22.2
• Domains: IG, IGv, PRY, SPRY
• Protein Families: Druggable Genome, Transmembrane
• MW: 101.3 kDa
• Summary: Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (1) and variant 3 encode the longest isoform (a).