WDR45 (NM_007075) Human Untagged Clone

SKU SC115710

WDR45 (untagged)-Human WD repeat domain 45 (WDR45), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: WDR45
• Synonyms: JM5; NBIA4; NBIA5; WDRX1; WIPI-4; WIPI4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115710 sequence for NM_007075 edited (data generated by NextGen Sequencing); ATGACTCAACAGCCACTTCGAGGAGTGACCAGCCTGCGTTTCAACCAAGACCAAAGCTGC; TTTTGCTGCGCCATGGAGACAGGTGTGCGCATCTACAACGTGGAGCCCTTGATGGAGAAG; GGGCATCTGGACCACGAGCAGGTGGGCAGCATGGGCTTGGTGGAGATGCTGCACCGCTCC; AACCTTCTGGCCTTGGTGGGCGGTGGTAGTAGTCCCAAGTTCTCAGAGATCTCAGCAGTG; CTGATCTGGGACGATGCCCGGGAGGGCAAGGACTCCAAGGAGAAGCTGGTGCTGGAGTTC; ACCTTCACCAAGCCAGTGCTTTCTGTGCGCATGCGCCATGACAAGATCGTGATCGTGCTG; AAGAACCGCATCTATGTGTACTCCTTCCCCGACAATCCCCGAAAGCTGTTTGAGTTTGAT; ACCCGGGACAACCCCAAGGGGCTCTGTGACCTCTGCCCCAGCCTGGAGAAGCAACTGCTA; GTGTTCCCGGGACACAAGTGTGGGAGTCTGCAACTTGTGGACCTGGCGAGCACAAAGCCT; GGCACCTCGTCTGCTCCATTCACGATCAATGCACATCAGAGTGACATAGCCTGTGTGTCT; CTAAACCAGCCAGGCACTGTAGTGGCCTCAGCCTCCCAGAAGGGTACCCTTATTCGCCTC; TTTGACACACAATCCAAGGAGAAACTGGTGGAGCTGCGCCGAGGCACTGACCCTGCCACC; CTCTACTGCATTAACTTCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAGTGATAAGGGT; ACTGTCCATATCTTTGCTCTCAAGGATACCCGCCTCAACCGCCGCTCCGCGCTGGCTCGC; GTGGGCAAGGTGGGGCCTATGATTGGGCAGTACGTGGACTCTCAGTGGAGCCTGGCGAGC; TTCACTGTGCCTGCTGAGTCAGCTTGCATCTGCGCCTTCGGTCGCAATACTTCCAAGAAC; GTCAACTCTGTCATTGCCATCTGCGTAGATGGGACCTTCCACAAATATGTCTTCACTCCT; GATGGAAACTGCAACAGAGAGGCTTTCGACGTGTACCTTGACATCTGTGATGATGATGAC; TTTTAA; ; Clone variation with respect to NM_007075.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007075 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGGAACCCCCTTC; GCCTCCGGTCCCCCAGCCAACCTTCCTCCGCTCCCCGCGCCCTCCCCGGAGAACCCCACT; GTCACTCGCGATGCTCCGAAGACCCGGGAACTAGGCGAGGAAGGCGGTGGCCGCCTTTTT; CCAGCTGGGAGGAACAATCCTGCACCATGACTCAACAGCCACTTCGAGGAGTGACCAGCC; TGCGTTTCAACCAAGACCAAAGCTGCTTTTGCTGCGCCATGGAGACAGGTGTGCGCATCT; ACAACGTGGAGCCCTTGATGGAGAAGGGGCATCTGGACCACGAGCAGGTGGGCAGCATGG; GCTTGGTGGAGATGCTGCACCGCTCCAACCTTCTGGCCTTGGTGGGCGGTGGTAGTAGTC; CCAAGTTCTCAGAGATCTCAGTGCTGATCTGGGACGATGCCCGGGAGGGCAACGACTCCA; ATGAGAAGCTGGTGCTGGAGTTCACCTTCACCAAGCCAGTGCTTTCTGTGCGCATGCGCC; ATGACAAGATCGTGATCGTGCTGAAGAACCGCATCTATGTGTACTCCTTCCCCGACAATC; CCCGAAAGCTGTTTGAGTTTGATACCCGGGACAACCCCAAGGGGCTCTGTGACCTCTGCC; CCAGCCTGGAGAAGCAACTGCTAGTGTTCCCGGGACACAAGTGCGGGAGTCTGCAACTTG; TGGACCTGGCGAGCACAAAGCCTGGCACCTCGTCTGCTCCATTCACGATCAATGCACATC; ACAGTGACATAGCCCTGTGTGTCTCTTAAACAGNNCAGCACTGTAGTGGCCTCAGACTTC; CAAAGGGTACCCTNATTCAACTCTTTGCACACATCCAAGGAGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007075 unedited; GGGGTTTTGGCGCCTTCTTACTCGATTGCTCACACAGGNGTACATCTCCTGCCTCAGCAA; GCTTTTAAAAAATCCCCATGTTGGATTATGGCACTCCCTCTGGGTGCATGCCTATTTCCT; CCAGTTACGGATCCCAAGGGTCGCTGACTTCCTGGGTCTCTGGCCTAGCCCTTGGGGCAC; ACAGTCATCAACAAATGCTGGGGGGAAGTGAGCTCTTTATTTAAACATAACTCTGCTGAG; TGGAAAGTGGGCACCAGCCCCATTAATGCTTGCTGGCTGGAGGCTTCCAAGCACGCCCCA; CTGCCAAAGCTAGCTCTGCATTCCTGTCACTGCAGGCTCCTAGCACAGCCCCCAGCGTCC; TTAAAAGTCATCATCATGACAGATGTCAACGTACACATCGAAAGCCTCTCTGTGGCATTC; TCCATCACGAGTGAAGACATATTTGCGGAATGACCCATCTACACATATGGCAATGACACA; TTTGACATGACTGTAAGCATCGCAACCGACTGCGCATATGCAAGCTGACTCATCACGCAC; AGAGAAGCTCGCCAGGCTCCACTGAGAGTCCACGTACTGACCAGTCATACGCCACACCTT; GCCCACTCGAGCCATCTCGAAGCGGCGGTTGATGCGGGTATACCTGAGAGCAAACTATGG; ACAGTACCCTTATCACTGGATGCTCACACGTATGTCGACTCGTGGCTGCAGTTAATGCAA; TCTAGGGCGGCAGGGCTATTGCCTCAGCACATGCTCACCAGACTCATCATCGGACTCGTG; TGTCAAAGATGCGAACATGACTACACCTGCTGCGATGCTAGAGACCACTACAGAGTCTAG; AGCTGCGTGATACACACACACGCTCATGTCACTCTGCATGAGCATCGCATCGTGCAACGG; GAGGTACGATGCGACATGTCGTATCCGCTCTCCTAGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007075
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007075.3, NP_009006.2
• RefSeq Size: 1925 bp
• RefSeq ORF: 1086 bp
• Locus ID: 11152
• UniProt ID: Q9Y484
• Cytogenetics: Xp11.23
• Domains: WD40
• Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents a longer transcript and encodes a longer isoform (1).