ELL2 (NM_012081) Human Untagged Clone

SKU SC115584

ELL2 (untagged)-Human elongation factor, RNA polymerase II, 2 (ELL2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ELL2
• Synonyms: MRCCAT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012081, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGGGGGGGACAGGGGGCCTGCGGGAGGAGCAGCGCTATGGGCTGTCGTGCGGACGGCTGGGGC; AGGACAACATCACCGTACTGCATGTGAAGCTCACCGAGACGGCGATCCGGGCGCTCGAGACTTACCAGAG; CCACAAGAATTTAATTCCTTTTCGACCTTCAATCCAGTTCCAAGGACTCCACGGGCTTGTCAAAATTCCC; AAAAATGATCCCCTCAATGAAGTTCATAACTTTAACTTTTATTTGTCAAATGTGGGCAAAGACAACCCTC; AGGGCAGCTTTGACTGCATCCAGCAAACATTCTCCAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATT; TATACAAGATAAAATTACAGTGTGTGCAACAAACGACTCGTATCAGATGACACGAGAAAGAATGACCCAG; GCAGAGGAGGAATCCCGCAACCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTAGGGAAAAGAG; TGCAAATTCGGAAAGCACCTCAAGCTGTTTCAGATACAGTTCCTGAGAGGAAAAGGTCAACCCCCATGAA; CCCTGCAAATACAATTCGAAAGACACATAGCAGCAGCACCATCTCTCAGAGGCCATACAGGGACAGGGTG; ATTCACTTACTGGCCCTGAAGGCCTACAAGAAACCGGAGCTACTTGCTAGACTCCAGAAAGATGGTGTCA; ATCAAAAAGACAAGAACTCCCTGGGAGCAATTCTGCAACAGGTAGCCAATCTGAATTCTAAGGACCTCTC; ATATACCTTAAAGGATTATGTTTTTAAAGAGCTTCAAAGAGACTGGCCTGGATACAGTGAAATAGACAGA; CGGTCATTGGAGTCAGTGCTCTCTAGAAAACTAAATCCGTCTCAGAATGCTGCAGGCACCAGCCGTTCAG; AATCTCCTGTATGTTCTAGTAGAGACGCTGTATCTTCTCCTCAGAAACGGCTTTTGGATTCAGAGTTTAT; TGATCCTTTAATGAATAAAAAAGCCCGAATATCTCACCTGACGAACAGAGTACCACCAACACTAAATGGT; CATTTGAATCCCACCAGTGAAAAATCTGCTGCAGGCCTCCCGCTGCCCCCTGCGGCTGCTGCCATCCCTA; CCCCTCCACCGCTGCCTTCAACCTATCTGCCCATCTCACATCCTCCTCAGATTGTAAATTCTAACTCCAA; CTCCCCTAGCACTCCAGAAGGCCGGGGGACTCAAGACCTACCTGTTGACAGTTTTAGTCAAAACGATAGT; ATCTATGAGGACCAGCAAGACAAATATACCTCTAGGACTTCTCTGGAAACCTTACCCCCTGGTTCCGTTC; TACTAAAGTGTCCAAAGCCTATGGAAGAAAACCATTCAATGTCTCACAAAAAGTCCAAAAAGAAGTCTAA; AAAACATAAGGAAAAGGACCAAATAAAAAAGCACGACATTGAGACTATTGAGGAAAAGGAGGAAGATCTT; AAGAGAGAAGAGGAAATTGCCAAGCTAAATAACTCCAGTCCAAATTCCAGTGGAGGAGTTAAAGAGGATT; GCACTGCCTCCATGGAACCTTCAGCAATTGAACTCCCAGATTATTTGATAAAATATATCGCTATCGTCTC; CTATGAGCAACGCCAGAATTATAAGGATGACTTCAATGCAGAGTATGATGAGTACAGAGCTTTGCATGCC; AGGATGGAGACTGTAGCTAGAAGATTTATCAAACTAGATGCACAAAGAAAGCGCCTTTCTCCAGGCTCAA; AAGAGTATCAGAATGTTCATGAAGAAGTCTTACAAGAATATCAGAAGATAAAGCAGTCTAGTCCCAATTA; CCATGAAGAAAAATACAGATGTGAATATCTTCATAACAAGCTGGCTCACATCAAAAGGCTAATAGGTGAA; TTTGACCAACAGCAAGCAGAGTCATGGTCCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012081 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGGCCCTAGCAGT; GGCGGCGGCTGCAGAAGCCCAAGCAGCCGCGGCCGCAGTGGAGGCTAGAGCCGGAGCGGC; GGCGGCGGCGGCACCCCGGGGAGTTTAAGATGGCGGCGGGGGGGACAGGGGGCCTGCGGG; AGGAGCAGCGCTATGGGCTGTCGTGCGGACGGCTGGGGCAGGACAACATCACCGTACTGC; ATGTGAAGCTCACCGAGACGGCGATCCGGGCGCTCGAGACTTACCAGAGCCACAAGAATT; TAATTCCTTTTCGACCTTCAATCCAGTTCCAAGGACTCCACGGGCTTGTCAAAATTCCCA; AAAATGATCCCCTCAATGAAGTTCATAACTTTAACTTTTATTTGTCAAATGTGGGCAAAG; ACAACCCTCAGGGCAGCTTTGACTGCATCCAGCAAACATTCTCCAGCTCTGGAGCCTCCC; AGCTCAATTGCCTGGGATTTATACAAGATAAAATTACAGTGTGTGCAACAAACGACTCGT; ATCAGATGACACGAGAAAGAATGACCCAGGCAGAGGAGGAATCCCCGCACCGAAGCACAA; AAGTTATCAAACCCGTTGGACCATATGTTAGGGAAAGAGTGCAAATTCGGAAAGCACCTC; AAGCTGTTTCAGATACAGTTCCTGAGAAGAAAAGGTCAACCCCCATGAACCCTTGCAATA; CAATTCGAAAGACACTTAGCAGCAGCACCATCTCTCAGAGGCCCATACAGGGACAGGGGG; ATTCACTTACTGGCCCTGAAGGCCTACAGGAAACCGGGACCTACTTGCTAGACTCCAGAA; GGATGTTGTCATTCANAAGACAANGACTCCCTGGAAGCATTTCTGCACCAGTTAGCCATT; TGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012081 unedited; ACGAGGCCGCATTCTAAGTCGAGTTTTTTTTTTTTTTTTTTCTGGAAGAAAACATCTTTA; TTAAAGACACTACCAAAATTTTGGCAAACATTTCAAAACAGATTTGTAAACATAATGCTT; CCCTTTTCAACTGGCAGCACTTTGAAAAGACAATACAATAAGACAATACAACTTGAATCA; ACATTAATTCAAAATCCTTATATTTTTGACCACATAACTTATGTTCCCACATGATAAAAC; AAGTGACAGTTTAAATCAATGTCAACAGATAAACTCCATGAAATGAAAGTTTGCGCTGTT; TGATGAATCACAGTATGCTATGGTTAAATATATCCACTCTTTTTTATATTCCTGGCACCA; GGATGAAAAAAAAAAATCTTTAAATATACCTCTTATGCAGGTAATATCCTCTTTGCATAT; CTGTCTTGAAAAAATACTTTATAGCAGTATATAAATAGGTCACCTACACATTCCATTTTA; TAATCATCGCGCCAAAACTATACATCTGTTTCATTCTCATGACATACCAATGTTTGCCCC; AACATTAACAAAGCTGACCAACACTCGCTGAAATGGAAACGCTCTTGTCTCTCCCAACAA; AAGTACCATCTTGCCACAAGAAACACCCCAAAAAACCTAAAACACCCTACAGTGACCCTC; GCCAGTTGTCAAACCTGAGCGCTTGGACCACTAAGAGTAACACCTTTTTTTCCTTAATGC; CCGCTTACATACACACCACCTGTGGACACATACAGATCGAGCACCCACTCTCAAAGCCGG; CAGGGAACACAATGCTCCCCTTGTTCTAACAAGTGGGAACCAGGTACTTAATGATTACCC; CCTTTGTACACAACTACCAACACCTTAAGGTACCATNGTAAGGGCGGTTAAATTGCAAGG; CGGATTTACTGGCCTAAACAAGTCACCCTGTCTTACAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_012081
• Insert Size: 3380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012081.3, NP_036213.1
• RefSeq Size: 3767 bp
• RefSeq ORF: 1923 bp
• Locus ID: 22936
• UniProt ID: O00472
• Cytogenetics: 5q15
• Protein Families: Transcription Factors
• Summary: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968). Plays a role in immunoglobulin secretion in plasma cells: directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression.[UniProtKB/Swiss-Prot Function]