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HS2ST1 (NM_012262) Human Untagged Clone

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SKU
SC115469
HS2ST1 (untagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HS2ST1
Synonyms dJ604K5.2; NFSRA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_012262 edited
ATGGGGCTCCTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTC
GCGGTGGCGATGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAG
CTAGAAAGGGCTATTGCAAGACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGAT
GGCCCTCGGCAAGATGCCACTTTAGATGAGGAAGAGGACATGGTGATCATTTATAACAGA
GTTCCCAAAACGGCAAGCACTTCATTTACCAATATCGCCTATGACCTGTGTGCAAAGAAT
AAATACCATGTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTCATTGCAAGAT
CAGGTGCGCTTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCAT
GGACACGTTTCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATT
AATGTCATAAGGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGA
GATGATTATAGACCAGGGTTACGGAGACGAAAACAAGGAGACAAAAAGACCTTTGATGAA
TGTGTAGCAGAAGGTGGCTCAGACTGTGCTCCAGAGAAGCTCTGGCTTCAAATCCCGTTC
TTCTGTGGCCATAGCTCCGAATGCTGGAATGTGGGAAGCAGGTGGGCTATGGATCAAGCC
AAGTATAACCTAATTAATGAATATTTTCTGGTGGGAGTTACTGAAGAACTTGAAGATTTT
ATCATGTTATTGGAGGCAGCATTGCCCCGGTTTTTCAGGGGTGCTACTGAACTCTATCGC
ACAGGAAAGAAATCTCATCTTAGGAAAACCACAGAGAAGAAACTCCCCACTAAACAAACC
ATTGCAAAACTACAGCAATCTGATATTTGGAAAATGGAGAATGAGTTCTATGAATTTGCA
CTAGAGCAGTTCCAATTCATCAGAGCCCATGCCGTTCGAGAAAAAGATGGAGACCTCTAC
ATCCTCGCACAAAACTTTTTCTATGAAAAGATTTACCCTAAGTCGAACTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012262 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTCATGGGNGCTC
CTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTCGCGGTGGCG
ATGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAGCTAGAAAGG
GCTATTGCAAGACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGATGGCCCTCGG
CAAGATGCCACTTTAGATGAGGAAGAGGACATGGTGATCATTTATAACAGAGTTCCCAAA
ACGGCAAGCACTTCATTTACCAATATCGCCTATGACCTGTGTGCAAAGAATAAATACCAT
GTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTCATTGCAAGATCAGGTGCGC
TTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCATGGACACGTT
TCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATTAATGTCATA
AGGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGAGATGATTAT
AGACCAGGGTTACGGAGACGAAAACAAGGAGACANAAAGACCTTTGATGAATGTGTAGCA
GAAGGTGGCTCAGACTGTGCTCCAGAGAAGCTCTGGCTTCAAATCCCGTTCTTCTGTGGC
CATAGCTCCGAATGCTGGAATGTGGGAAGCANGTGGGCTATGGNATCAAGCCAGTATAAC
CTAATAATGAATATTNTCTGGTGGGGAGTACTGAAGAAACTTGAGATTTATCATGGTATT
GGNAGGCAGCATGCCCCCGTTTTTCAGGGGTGCTACTGAACTCTATCGACAGGAAAGAAT
CTCATCTANGAAACCACAGAGAGAAACTCCCACTAACAACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012262 unedited
NNTTTCTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTATTTA
TCAAACACAAATTTATATCAAGATTAACTTTTTCAACCAGCTTACAAGCAGACTTTTCCC
TTTCTTTGTTAAAAAATGAATGGTATGAAACTATATGTTAAAGATGTTTCTATAATACCT
TACATTTACATAGCGCTTTACAATTTTCAAAGTGCTTTCACATGCCATCTCATTTAATCC
TCACAACAGCCCTGTGAGGTAGGTACAGCAGGTATTATTATCCTCATTTCAAGATGAGGA
AATTGAGGCAAAGAGAGGTTAAGTGACTTGCCCAAGATCACACAGCTGTAAGTGCTAGAG
CTAGGACCTGAAATCAAGTTTTTTGACTCCTTGTCCAGTACTCATTCCACTGTAATTACT
GCCTCAAGTTATGAATAATGAACTGTGTATCAAAATTGAAAGCTTACTGAAGTTCATTTC
AGTGATGTCTTAACAGTAAAGTTAAATGAGAAATACAAGAACAGTAGGTCTGATGGTTTT
ACTTGACATAAAATTGGTGCAAAACCACTTTTGTCTAATTACTTGTAACCTTTTGGTCCA
AATACATAAAATATGCAATATTTACAGCATTTTCCTTGTTTTGTAAATGCAATGCTTTAA
AACACATTATAAAGCACCTTAGTAAAAATAGTCTACAGGTAAAAGTACAGAAGAAGTANG
GAAAATTCTACCCATACCACAGCTCACTAATGGAGTCATAATACCGAGTCACCGATATCT
CAGTTTACAGCTTTACAAATTACCCATGGAGAAACAATACTAAACTTACTTTTTTTGAAA
TAAAGGGGGAAAAACCTAACTAAAACCTCAGACAATCTCAATATTCCTTTGAGAATTCTT
CCCCATTTTAAAAATTGAGGGAGGCACGAATGAGAAAAACCGCGTTTGAGCGCTTAACTT
TTTTACAA
Restriction Sites NotI-NotI |
ACCN NM_012262
Insert Size 2450 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012262.2, NP_036394.1
RefSeq Size 6708 bp
RefSeq ORF 1071 bp
Locus ID 9653
UniProt ID Q7LGA3
Cytogenetics 1p22.3
Domains HS2ST
Protein Pathways Heparan sulfate biosynthesis
Summary Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. provided by RefSeq, Aug 2008
Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_012262" in other vectors (6)
SKU Description Size Price
RC223657 HS2ST1 (Myc-DDK-tagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1 10 ug
$457.00
RC223657L1 Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC223657L2 Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC223657L3 Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC223657L4 Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG223657 HS2ST1 (tGFP-tagged) - Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1 10 ug
$489.00 $657.00

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