PITPNB (NM_012399) Human Untagged Clone

SKU SC115416

PITPNB (untagged)-Human phosphatidylinositol transfer protein, beta (PITPNB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PITPNB
• Synonyms: PI-TP-beta; PtdInsTP; VIB1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012399, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGTGCTGATCAAGGAATTCCGTGTGGTTTTGCCATGTTCTGTTCAGGAGTATCAGGTTGGGCAGCTTT; ACTCTGTTGCAGAAGCTAGTAAGAATGAGACTGGTGGTGGAGAAGGAATTGAAGTCTTAAAGAATGAACC; TTATGAGAAGGATGGAGAAAAGGGACAGTATACGCACAAAATTTATCACCTAAAGAGCAAAGTGCCTGCA; TTCGTGAGGATGATTGCTCCCGAGGGCTCCTTGGTGTTTCATGAGAAAGCCTGGAATGCGTACCCCTACT; GTAGAACAATTGTAACGAATGAATATATGAAAGATGATTTCTTCATTAAAATCGAAACATGGCACAAACC; AGACTTGGGAACATTAGAAAATGTACATGGTTTAGATCCAAACACATGGAAAACTGTTGAAATTGTCCAT; ATAGATATTGCAGATAGAAGTCAAGTTGAACCAGCAGACTACAAAGCTGATGAAGACCCAGCATTATTCC; AGTCAGTCAAGACCAAGAGAGGCCCTTTGGGACCCAACTGGAAGAAGGAGCTGGCAAACAGCCCTGACTG; TCCCCAGATGTGTGCCTATAAGCTGGTGACCATCAAATTCAAGTGGTGGGGACTGCAAAGCAAAGTAGAA; AACTTCATTCAAAAGCAAGAAAAACGGATATTTACAAACTTCCATCGCCAGCTTTTTTGTTGGATTGACA; AGTGGATCGATCTCACGATGGAAGACATTAGGAGAATGGAAGACGAGACTCAGAAAGAACTAGAAACAAT; GCGTAAGAGGGGTTCCGTTCGAGGCACGTCGGCTGCTGATGTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012399 unedited; GTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGGCGGCGGC; GGTGGTATCGGCGGCAGCTGTGAGGGGGTTCCGGGAAGATGGTGCTGATCAAGGAATTCC; GTGTGGTTTTGCCATGTTCTGTTCAGGAGTATCAGGTTGGGCAGCTTTACTCTGTTGCAG; AAGCTAGTAAGAATGAGACTGGTGGTGGAGAAGGAATTGAAGTCTTAAAGAATGAACCTT; ATGAGAAGGATGGAGAAAAGGGACAGTATACGCACAAAATTTATCACCTAAAGAGCAAAG; TGCCTGCATTCGTGAGGATGATTGCTCCCGAGGGCTCCTTGGTGTTTCATGAGAAAGCCT; GGAATGCGTACCCCTACTGTAGAACAATTGTAACGAATGAATATATGAAAGATGATTTCT; TCATTAAAATCGAAACATGGCACAAACCAGACTTGGGAACATTAGAAAATGTACATGGTT; TAGATCCAAACACATGGAAAACTGTTGAAATTGTCCATATAGATATTGCAGATAGAAGTC; AAGTTGAACCAGCAGACTACAAAGCTGATGAAGACCCAGCATTATTCCAGTCAGTCAAGA; CCAAGAGAGGCCCTTTGGGACCCAACTGGAAGAAGGAGCTGGCAAACAGCCCTGACTGTC; CCCAGATGTGTGCCTATAAGCTGGTGACCATCAAATTCAAGTGGTGGGGACTGCAAAGCA; AAGTAGAAAACTTCATTCACAAGCAAGAAAAACGGATATTTACAAACTTCCATCGCCAGC; TTTTTTGTTGGATTGACAAGTGGATCGATCTCCCGATGGAAGACATTAGGAGAATGGAAG; ACGAGACTTCGAAAGGACTAGAAACAATGCGTTAGGAGGGTTTCCGTTCCGAGCACGTCG; GCTGCTGATGTCTAGATGAGTCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012399 unedited; ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGATCAAATTTATTT; TTTGTTTAAATTTCATTTACTTTGTTTTACTGTAATTTACACAAGAGACTGGCAAGTAAA; CTAGGTATTTTACATTCACCACACATTCCCTCAAATCTCCACAGTTGTTAGAAAAACATT; AAAATCCATGCGCCGGGCTCTCATTTCCATGTGCGCCTAAGCTCCCAATGATACTACAGA; TGCCAGCGAGAGTTAAGTTCATTAAAAGGAGAGGGCTAGACTCTTTATTTCACAAAATTA; GCAATAATCTTCCTCGCACCAAACACTTTGCAGACAATGATTATGCTCTGACAAAACCTA; TCTTACAACAGTGCCCAGAGAGTAAACATCAGTCTTTATCCTGAGTACACAAAGGATGTA; TGAAATGTGGGTTTTGTTGCTGAGGATAACAGGGTATTGCAATGCAGTAGTGATCCTACA; CATCCTTCCTTGTCTGATTCACTGAAATCACTCCAGTGGGGGAGGGATGCAGGACATGTG; GGAAAACGAGGAGCAGGCAGGGCAGGACTGTCAGCAAATCAGATTACTAATGTGATTTGA; CTGCANGGGTTTGGCGTGTTTTAGGAGGGACAGGAGGTTACAAAAGACAGTTTGTGACCT; GAAAGCTTCACAATATAATTCTATTCAAGCTCCCAGACTGACAGAGGAAAAACTGGATTC; AGACAGAGAAAGCTGATAAAAATCTTACCTAGCTATTGTTCCTTCCCTTTAATAAAAAAT; AAAATAAAATTAAAAAAGTGGCAGCCCGTTTCTGACCAGCCCTCTCATGAATGCCTATTC; AAAATCCTGGCCTGAGGAACGCCCTACTTGGAGAGCCCATCCCAGTCAGATGGTCCCCAA; TTTCTTTGGCGGGGGCCTCCTTACCGGCACGCAGGCAAACTCACAACTGATTTTAAACTG; TTCCTATTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_012399
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012399.3, NP_036531.1
• RefSeq Size: 2941 bp
• RefSeq ORF: 816 bp
• Locus ID: 23760
• UniProt ID: P48739
• Cytogenetics: 22q12.1
• Domains: IP_trans
• Summary: This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]; Transcript Variant: This variant (sp1) encodes the shortest isoform (1).