Tin2 (TINF2) (NM_012461) Human Untagged Clone

SKU SC115364

TINF2 (untagged)-Human TERF1 (TRF1)-interacting nuclear factor 2 (TINF2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Tin2
• Synonyms: DKCA3; TIN2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115364 sequence for NM_012461 edited (data generated by NextGen Sequencing); ATGGCTACGCCCCTGGTGGCGGGTCCCGCAGCTCTACGCTTCGCCGCCGCGGCTAGCTGG; CAGGTTGTGCGCGGACGCTGCGTGGAACATTTTCCGCGAGTACTGGAGTTTCTGCGATCT; CTGCGCGCTGTTGCCCCTGGCTTGGTTCGCTACCGGCACCACGAACGCCTTTGTATGGGC; CTAAAGGCCAAGGTGGTGGTGGAGCTGATCCTGCAGGGCCGGCCTTGGGCCCAAGTCCTG; AAAGCCCTGAATCACCACTTTCCAGAATCTGGACCTATAGTGCGGGATCCCAAGGCTACA; AAGCAGGATCTGAGGAAGATTTTGGAGGCACAGGAAACTTTTTACCAGCAGGTGAAGCAG; CTGTCAGAGGCTCCTGTGGATTTGGCCTCGAAGCTGCAGGAACTTGAACAAGAGTATGGG; GAACCCTTTCTGGCTGCCATGGAAAAGCTGCTTTTTGAGTACTTGTGTCAGCTGGAGAAA; GCACTGCCTACACCGCAGGCACAGCAGCTTCAGGATGTGCTGAGTTGGATGCAGCCTGGA; GTCTCTATCACCTCTTCTCTTGCCTGGAGACAATATGGTGTGGACATGGGGTGGCTGCTT; CCAGAGTGCTCTGTTACTGACTCAGTGAACCTGGCTGAGCCCATGGAACAGAATCCTCCT; CAGCAACAAAGACTAGCACTCCACAATCCCCTGCCAAAAGCCAAGCCTGGCACACATCTT; CCTCAGGGACCATCTTCAAGGACGCACCCAGAACCTCTAGCTGGCCGACACTTCAATCTG; GCCCCTCTAGGCCGACGAAGAGTTCAGTCCCAATGGGCCTCCACTAGGGGAGGCCATAAG; GAGCGCCCCACAGTCATGCTGTTTCCCTTTAGGAATCTCGGCTCACCAACCCAGGTCATA; TCTAAGCCTGAGAGCAAGGAAGAACATGCGATATACACAGCAGACCTAGCCATGGGCACA; AGAGCAGCCTCCACTGGGAAGTCTAAGAGTCCATGCCAGACCCTGGGGGGAAGGGCTCTG; AAGGAGAACCCAGTTGACTTGCCTGCCACAGAGCAAAAGGAGTGA; ; Clone variation with respect to NM_012461.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012461 unedited; GGGTTGCGNATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGC; GACGTTTAAAGCTGAGCGACCCAGTGCCACTGGAGACGGTCAGCTTCTCCACTCAGGCTC; CTCCAGCCCGAGCCAGAAGACCCCCTCCCCCAGAATTCTGGGGGCCGATGGAAGGGAGCC; GAGTCAGATCGCGAGGTACCCAGAGCCGACAGACCGGAGCGACAGGGAGTTGCCAGAAGC; CCCGCCCCTAGGAGTGATCGGAAAGCCTCACCCATCCGGGTGAGGAACCCGGAGGGACCG; CCTCCGGGCGGAGCCCGCCGACCATGGCTACGCCCCTGGTGGCGGGTCCCGCAGCTCTAC; GCTTCGCCGCCGCGGCTAGCTGGCAGGTTGTGCGCGGACGCTGCGTGGAACATTTTCCGC; GAGTACTGGAGTTTCTGCGATCTCTGCGCGCTGTTGCCCCTGGCTTGGTTCGCTACCGGC; ACCACGAACGCCTTTGTATGGGCCTAAAGGCCAAGGTGGTGGTGGAGCTGATCCTGCAGG; GCCGGCCTTGGGCCCAAGTCCTGAAAGCCCTGAATCACCACTTTCCAGAATCTGGACCTA; TAGTGCGGGATCCCAAGGCTACAAAGCAGGATCTGAGGAAGATTTTGGAGGCACAGGAAA; CTTTTTACCAGCAGGTGAAGCAGCTGTCAGAGGCTCCTGTGGATTTGGCCTCGAAGCTGC; AGGAACTTGAACAAGAGTATGGGGAACCCTTTCTGGCTGCCATGGAAAAGCTGCTTTTTG; AGTACTTGTGTCAGCTGGAGAAGCACTGCCTACACCGCAGGCACAGCAGCTTCAGGATGT; GCTGAGTTGGATGCAGCCTGGAGTCTCTATCACCTCTTCTCTTGCCTGGNAGACATATGG; TGTGGACATGGNNGTGGCTGCTTNCAGAGTGCTCTGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012461 unedited; NTAACTCTGGACCGCGGCCGCAATCTANGATCGGTNTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTAAACAAATCCAAAGTTTAATTATTAAGGATTACAAATATTTTTAGCAG; GGTAGTTAGGCAATCCAAGCCTGGACTTCCACTTCATTCCTACTAAACTACTTGCAAAGC; TGAGGAGGCAGGAAACTAAAGTACAGAGAGCATTTTAGTTCTATCACAAAGGTCTAAAAC; TGTCTCTACAGTCACAGGAAAAAACAGGTATGGCACCGGGGCCAAAAGGGGGTAGGTATT; CACAGAGAGGGGGTATCAAGGGGGCAAACTTTGTCTTCTGATAGTTTTCCAAAAATTCCT; GTAAAAAAGGGAGCAGGGAGAGCCTACTATCCGAAACCATTCCCTGAACCCTTTGAATTC; TGAAGCATGGGGATTCCTCAGGCTTGGTCTACCCATCCCCTTTCCCAGCTTTCGGCTCCT; TCCCACTCCCCTTTCCTTCCCCCGGGCCATTTTCTTCCCCTCAGAGTCCAAAACAAGGGC; CCTTGGTAATCGCGGAACTCCCCCAAACGGAGCCCCGCTACGGGGGAACCCCGCCGTGGG; GTTCCGCGCCGCAGCGGCAGACGCACTGTTTTATCCTCCGCCAACCCCCCCCTTGGCCAC; TCTCTCCACGACGCGTGGTCCCTTTTCGAGGCTTCCATGTCGTAATATCACTCCCCCACT; TCATCTCGAGCCCCCCCCACTCCCCTCCTCTTTATCTTTCCTCTCTCGGCTAGCGTTTCC; CCCAGCTCCCGCCCCCCGTCCCCATTCCTCACCGCGCCTCCTCCCTCTCCCAACTTTTCC; GCTCTCCCCCCCCCTCCCCACCCCCGCCCCCTTTCACCCCCCCCCTCTGTGTATCCCCCT; CTCCCCGACCCTGTTCTACATTCCTGATCCCTCACAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_012461
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012461.1, NP_036593.1
• RefSeq Size: 2095 bp
• RefSeq ORF: 1065 bp
• Locus ID: 26277
• UniProt ID: Q9BSI4
• Cytogenetics: 14q12
• Summary: This gene encodes one of the proteins of the shelterin, or telosome, complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex, and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome. [provided by RefSeq, Mar 2010]; Transcript Variant: This variant (2) contains additional segments at its 3' end, which results in an early stop codon and a unique 3' UTR, compared to variant 1. It encodes isoform 2, also known as TIN2S, which has a shorter C-terminus when compared to isoform 1.