DNA Polymerase lambda (POLL) (NM_013274) Human Untagged Clone

SKU SC115324

POLL (untagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNA Polymerase lambda
• Synonyms: BETAN; POLKAPPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115324 sequence for NM_013274 edited (data generated by NextGen Sequencing); ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCA; TCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGG; CTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTC; TTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAGGTGTC; ACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCAGACTA; CCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCCTTCAG; GAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACTTGGAC; CATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCC; CTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAA; AAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGAT; GGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACTACCCC; CCCTCCCTTGAGGGAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAGTGGGTC; TGTGCACAGCCCTCAAGCCAGAAGGCGACCAATCACAACCTCCATATCACAGAGAAGCTG; GAAGTTCTGGCCAAAGCCTACAGTGTTCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCC; AAGGCCATCAATGCCCTCAAGAGCTTCCATAAGCCTGTCACCTCGTACCAGGAGGCCTGC; AGTATCCCTGGGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGG; CATTTGCGGAAGCTGGACCATATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAAC; ATCTGGGGAGCTGGGACCAAGACTGCCCAGATGTGGTACCAACAGGGCTTCCGAAGTCTG; GAAGACATCCGCAGCCAGGCCTCCCTGACAACCCAGCAGGCCATCGGCCTGAAGCATTAC; AGTGACTTCCTGGAACGTATGCCCAGGGAGGAGGCTACAGAGATTGAGCAGACAGTCCAG; AAAGCAGCCCAGGCCTTTAACTCCGGGCTGCTGTGTGTGGCATGTGGTTCATACCGACGG; GGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGCYGGTCCCAC; CGGGGTATCTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGAC; TTGGTGAGCCAAGAGGAGAATGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCA; GGGCCAGGGCGGCGGCACCGGCGCCTGGACATCATCGTGGTGCCCTATAGCGAGTTTGCC; TGTGCCCTGCTCTACTTCACCGGCTCTGCACACTTCAACCGCTCCATGCGAGCCCTGGCC; AAAACCAAGGGCATGAGTCTGTCAGAACATGCCCTCAGCACTGCTGTGGTCCGGAACACC; CATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCCACTCCCACTGAGAAGGATGTCTTC; AGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGGTGA; ; Clone variation with respect to NM_013274.3; 661 a=>c;1224 t=>c;1312 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013274 unedited; NCTTGCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCAA; TTCTGGATTTCCACGTGCAGCAGCCCTGACCACGCTCCAATAGGCCGGGATCCAGCCATA; CTTCAATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTG; ATGCATCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAG; AGTGGCTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAG; AACTCTTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAG; GTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCA; GACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCC; TTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACT; TGGACCATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATG; AGGCCCTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTC; CCCAAAAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCA; GTGATGGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACT; ACCCCCCCTCCCTTGAGGGNAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAG; TGGGGTCTGTGCACAGCCCTCAAGCCAGAAGCGACCAATCACAACCTNCATATCACAGAG; AAGCTGGNAAGTTCTGGGCCAAGCCTACAGTGNTCAGGNAGACCAAGTGGNAGGCCCNNT; GGCTTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013274 unedited; NTTCTACTTGNACCCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGGTTC; AATGTCAAATGCACTTTACTGGGCCCCTGGGCCTCCCAACACTGTCCTCACCAAGGCTGG; GGCACCCCATTCCCAGCACCACCAGCTGACCGGGAAGCAGGACGGATAGAGGGAACACAT; GTGGAATGGCTGTTCTCCTACCCCAAAGGTGCCGGATGATGTTGACAGCACGGCTGCTTC; CCTCTCCCCTGCAGCTTCTGTCCCCACCCAGCAGCTTGGCCTTTCCCATAGCAGGGAAGG; GGGATACCTGGGGCTTCAAACCAGCCACCTGCTCCTGCTTAAAATGGGGCAAGGGGCCAT; TTGCAAAATTCTTCGAGGGGCAGTGGTGAGGTAAAAGGGGTGGCAGCCTGCTCCTGTCTG; GGAGAGGGCTGGGCAGACACTGGGAGCCGGGCAGACACTGGGAGCCGGGCTGGCTCTTGC; TTCAGGCCCAGAGCCCTGGGCCCTGCTCGCTGAGGAAGCTGGTGGTTGGAGCGGCGAGGT; TCAACCAGCTGAGGCTGGAAGGAGTACTGGGTGGCCAGGAGGGGTAGCCAGTCCAACTCG; GCTCTCCTCAGCACCCCCAGCCATGGGTCACCAGTCCCGCTCAGCAGGTTCTCGGTAGGG; GAGGCCTAAGAGCCTGAAGACATCCTTCTCAGTGGGAGTGGGCAGCACTCGGCCAGGCCC; CACCTTGCAGCCATGGGGTGTTCCGGACCACAGCAGTGCTGAGGGCATGTTCTGACAGAC; TCATGCCCTTTGGTTNTGGCCCAGGCTCGCATGGAGCGGNTGAAGTGTGCAAAGCCGGTG; AAGTAAAGCAGGGCACAGGCAAACTCGCTATAGGGCACCACGATGATGTCCAGGCGCCGG; TGCCGNCGCCTGGCCCTGGGAGCCNGCACACCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_013274
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013274.2, NP_037406.1
• RefSeq Size: 2702 bp
• RefSeq ORF: 1728 bp
• Locus ID: 27343
• UniProt ID: Q9UGP5
• Cytogenetics: 10q24.32
• Domains: POLXc
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, Non-homologous end-joining
• Summary: This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).